KIAA0767

Product ID	ORK01993
Accession No	AB018310
Description	GRAM domain containing 4
Clone name	aj00389
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4481 bp) Predicted protein sequence (598 aa)
HaloTag ORF Clone	FHC01993
Flexi ORF Clone	FXC01993
Source	Human brain (amygdala)
Rouge ID	mKIAA0767 by Kazusa Mouse cDNA Project
Note	We replaced hk04874, former representative clones for KIAA0767 with aj00389. (2005/08/06)

Length: 4481 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2531 bp
Genome contig ID	gi89161203f_45294963
PolyA signal sequence (AATAAA,-24)	+--------+--------+----*----+---- ATTTTTATTTAAATAAACCTAAAATGCTTTGTGCT
Flanking genome sequence (159383 - 159432)	----+--------+--------+--------+--------+----* AAGGCTCAGGGCTGTCTGCCTCCTTGGCAGGGCCACACGCATTGTAGGCG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	22	f	45394963	45454344	19	99.3	Perfect prediction

Length: 598 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q6IC98	0	100.0	GRAM domain-con...	Homo sapiens
Q8CB44	0	93.0	GRAM domain-con...	Mus musculus
XP_001078454	0	93.3	similar to deat...	Rattus norvegicus
XP_235571	7.7e-217	93.3	similar to deat...	Rattus norvegicus
XP_001489246	8.6e-213	94.1	GRAM domain con...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004182	465	543	PF02893	GRAM
HMMSmart	IPR004182	465	543	SM00568	GRAM

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	272	PLFLFLAILRLSLNYLIARGWRI	294	PRIMARY	23
	2	348	MWVQPEITQKLYVALWAAFLASC	370	SECONDARY	23
	3	375	RLVGLAVGLYAGIKFFLIDFIF	396	SECONDARY	22

Primer_f	GACAATGGCCACACCTCTCTC
Primer_r	AGGGACTCGAACTAAACCACC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	UniGene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-