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Gene/Protein Characteristic Table for FLJ00010

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Product ID	ORK05034
Accession No	AK024421
Clone name	as00010
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4126 bp) Predicted protein sequence (772 aa)
Source	Human spleen

Length: 4126 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 772 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004841	9	394	PF00324	Amino acid permease-associated region
ProfileScan	IPR002293	125	295	PS50285	Amino acid/polyamine transporter I

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	25	NLLYGSLLLGLVGGVCTLGAGLY	47	PRIMARY	23
	2	52	FLTFLLVSGSLASVLISFVAVGP	74	PRIMARY	23
	3	118	TTGAVMNFASVFAVLFNGCTGIM	140	SECONDARY	23
	4	158	LGTIVAVAYTFFVYVLLFFLSSF	180	PRIMARY	23
	5	199	LWPPLVLIGIYATALSASMSSLI	221	SECONDARY	23
	6	250	GNPWAAVLYSWGLVQLVLLAGKL	272	SECONDARY	23
	7	278	VVTVFYLVAYAAVDLSCLSLEWA	300	PRIMARY	23
	8	323	SCLLMMFLISPGAAGGSLLLMGL	345	PRIMARY	23

RT-PCR-ELISA

Experimental conditions

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