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Gene/Protein Characteristic Table for FLJ00015

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Product ID	ORK05036
Accession No	AK024426
Clone name	as00015
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4737 bp) Predicted protein sequence (307 aa)
Source	Human spleen

Length: 4737 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 307 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000832	17	269	PF00002	G-protein coupled receptor family 2 (secretin-like)
ProfileScan	IPR000832	20	266	PS50261	G-protein coupled receptor family 2 (secretin-like)
	NULL	28	110	PS50319	NULL

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	22	VITYMGLSVSLLCLLLAALTFLL	44	PRIMARY	23
	2	54	SLHLQLSLCLFLAHLLFLVAIDQ	76	PRIMARY	23
	3	85	IIAGTLHYLYLATLTWMLLEALY	107	PRIMARY	23
	4	130	LMFPVGYGVPAVTVAISAASRP	151	SECONDARY	22
	5	174	GPVCAIFSVNLVLFLVTLWILK	195	SECONDARY	22
	6	218	ATAQLFILGCTWCLGILQVGPAA	240	PRIMARY	23
	7	246	LFTIINSLQGVFIFLVYCLLSQQ	268	PRIMARY	23

RT-PCR-ELISA

Experimental conditions

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