FLJ00021

Product ID	ORK07132
Accession No	AK074029
Clone name	as00021
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4676 bp) Predicted protein sequence (508 aa)
HaloTag ORF Clone	FHC07132
Flexi ORF Clone	FXC07132
Source	Human spleen
Rouge ID	mFLJ00021 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Length: 508 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84855	0	100.0	FLJ00021 protei...	Homo sapiens
Q8NE00	0	100.0	Transmembrane p...	Homo sapiens
AAH37278	0	99.8	Transmembrane p...	Homo sapiens
BAF83848	0	99.6	unnamed protein...	Homo sapiens
Q5RCV1	4.1e-216	98.8	Transmembrane p...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR002422	22	502	PF01490	Amino acid/polyamine transporter II
ProfileScan	IPR002422	25	457	PS50286	Amino acid/polyamine transporter II

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	23	LYSSYVGLVYMFNLIVGTGALTM	45	PRIMARY	23
	2	57	SLVLLVFLGFMSFVTTTFVIEAM	79	PRIMARY	23
	3	162	FCIIVYLYGDLAIYAAAVPFSLM	184	PRIMARY	23
	4	219	AYRIYLAIFTLLLGPFTFFDVQK	241	SECONDARY	23
	5	247	ILTSLMRWIAFAVMIVLALIRIG	269	PRIMARY	23
	6	321	LDYVLILAFYGLLSFTAIFCFRG	343	PRIMARY	23
	7	361	GLAAVRFFLGLFPVFTISTNFPI	383	SECONDARY	23
	8	405	WVVDRVVFPTITLVPPVLVAFCT	427	SECONDARY	23
	9	434	VGITGAYAGTGIQYVIPAFLVYH	456	SECONDARY	23
	10	482	WVGFVLLWAFSCFIFVTANIILS	504	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test