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Gene/Protein Characteristic Table for FLJ00070

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Product ID	ORK01033
Accession No	AK024477
Clone name	as00070
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4439 bp) Predicted protein sequence (1382 aa)
HaloTag ORF Clone	FHC01033
Flexi ORF Clone	FXC01033
Source	Human spleen
Rouge ID	mFLJ00070 by Kazusa Mouse cDNA Project

Length: 4439 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 1382 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001438	695	706	PR00010	Type II EGF-like signature
	IPR001438	818	825	PR00010	Type II EGF-like signature
	IPR001438	967	977	PR00010	Type II EGF-like signature
HMMPfam	IPR006209	192	219	PF00008	EGF-like domain
	IPR002212	366	409	PF00683	Matrix fibril-associated
	NULL	434	473	PF07645	NULL
	IPR002212	492	533	PF00683	Matrix fibril-associated
	NULL	695	737	PF07645	NULL
	NULL	739	771	PF07645	NULL
	NULL	782	821	PF07645	NULL
	NULL	823	862	PF07645	NULL
	NULL	864	903	PF07645	NULL
	IPR006209	868	903	PF00008	EGF-like domain
	NULL	905	943	PF07645	NULL
	NULL	945	986	PF07645	NULL
	IPR006209	949	986	PF00008	EGF-like domain
	IPR002212	1006	1049	PF00683	Matrix fibril-associated
	NULL	1072	1113	PF07645	NULL
	NULL	1115	1154	PF07645	NULL
	IPR006209	1119	1154	PF00008	EGF-like domain
	NULL	1161	1200	PF07645	NULL
	IPR002212	1225	1270	PF00683	Matrix fibril-associated
	NULL	1333	1376	PF07645	NULL
HMMSmart	IPR006210	191	220	SM00181	Type I EGF
	IPR001881	192	220	SM00179	EGF-like calcium-binding
	IPR001881	434	474	SM00179	EGF-like calcium-binding
	IPR006210	437	474	SM00181	Type I EGF
	IPR001881	653	694	SM00179	EGF-like calcium-binding
	IPR006210	656	694	SM00181	Type I EGF
	IPR001881	695	738	SM00179	EGF-like calcium-binding
	IPR006210	698	738	SM00181	Type I EGF
	IPR001881	739	781	SM00179	EGF-like calcium-binding
	IPR006210	742	781	SM00181	Type I EGF
	IPR001881	782	822	SM00179	EGF-like calcium-binding
	IPR006210	785	822	SM00181	Type I EGF
	IPR001881	823	863	SM00179	EGF-like calcium-binding
	IPR006210	826	863	SM00181	Type I EGF
	IPR001881	864	904	SM00179	EGF-like calcium-binding
	IPR006210	867	904	SM00181	Type I EGF
	IPR001881	905	944	SM00179	EGF-like calcium-binding
	IPR006210	908	944	SM00181	Type I EGF
	IPR001881	945	987	SM00179	EGF-like calcium-binding
	IPR006210	948	987	SM00181	Type I EGF
	IPR001881	1072	1114	SM00179	EGF-like calcium-binding
	IPR006210	1075	1114	SM00181	Type I EGF
	IPR001881	1115	1155	SM00179	EGF-like calcium-binding
	IPR006210	1118	1155	SM00181	Type I EGF
	IPR001881	1161	1201	SM00179	EGF-like calcium-binding
	IPR006210	1164	1201	SM00181	Type I EGF
	IPR001881	1296	1332	SM00179	EGF-like calcium-binding
	IPR006210	1299	1332	SM00181	Type I EGF
	IPR001881	1333	1377	SM00179	EGF-like calcium-binding
	IPR006210	1336	1377	SM00181	Type I EGF
ProfileScan	IPR000694	25	84	PS50099	Proline-rich region
	NULL	83	246	PS50315	NULL
	NULL	434	474	PS50034	NULL
	NULL	653	694	PS50034	NULL
	NULL	657	973	PS50311	NULL
	NULL	695	738	PS50034	NULL
	NULL	739	781	PS50034	NULL
	NULL	782	822	PS50034	NULL
	NULL	823	863	PS50034	NULL
	NULL	864	904	PS50034	NULL
	NULL	905	944	PS50034	NULL
	NULL	945	987	PS50034	NULL
	NULL	1072	1114	PS50034	NULL
	NULL	1115	1155	PS50034	NULL
	NULL	1161	1201	PS50034	NULL
	NULL	1333	1377	PS50034	NULL
ScanRegExp	IPR006209	208	219	PS00022	EGF-like domain
	IPR001881	434	458	PS01187	EGF-like calcium-binding
	IPR000152	449	460	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR006209	678	693	PS01186	EGF-like domain
	IPR001881	695	720	PS01187	EGF-like calcium-binding
	IPR000152	711	722	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR001881	739	764	PS01187	EGF-like calcium-binding
	IPR000152	755	766	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR001881	782	806	PS01187	EGF-like calcium-binding
	IPR001881	823	847	PS01187	EGF-like calcium-binding
	IPR000152	838	849	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR006209	847	862	PS01186	EGF-like domain
	IPR001881	864	888	PS01187	EGF-like calcium-binding
	IPR000152	879	890	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR006209	888	903	PS01186	EGF-like domain
	IPR001881	905	929	PS01187	EGF-like calcium-binding
	IPR000152	920	931	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR001881	945	971	PS01187	EGF-like calcium-binding
	IPR000152	962	973	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR006209	971	986	PS01186	EGF-like domain
	IPR001881	1072	1098	PS01187	EGF-like calcium-binding
	IPR000152	1089	1100	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR006209	1098	1113	PS01186	EGF-like domain
	IPR001881	1115	1139	PS01187	EGF-like calcium-binding
	IPR000152	1130	1141	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR001881	1161	1185	PS01187	EGF-like calcium-binding
	IPR000152	1176	1187	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR006209	1185	1200	PS01186	EGF-like domain
	IPR006209	1316	1331	PS01186	EGF-like domain
	IPR001881	1333	1361	PS01187	EGF-like calcium-binding
	IPR000152	1352	1363	PS00010	Aspartic acid and asparagine hydroxylation site
	IPR006209	1361	1376	PS01186	EGF-like domain

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	98	AGAAGLLALLLLLLLLLGLGGRV	120	PRIMARY	23

RT-PCR-ELISA

Experimental conditions

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