Gene/Protein Characteristic Table for KIAA0454
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Product ID ORK05601
Accession No AB007923
Description phosphodiesterase 4D interacting protein
Clone name bf02274
Vector information
The cDNA fragment was inserted at the XhoI-SacI site of the ...
cDNA sequence DNA sequence (8018 bp)
Predicted protein sequence (2296 aa)
Source Human adult brain
Rouge ID mKIAA0454 by Kazusa Mouse cDNA Project
Note We replaced hg00502, former representative clones for KIAA0454 with bf02274. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 8018 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 1030 bp
Genome contig ID gi89161185r_143462785
PolyA signal sequence
(AATAAA,-19)
+----*----+----*----+----*----+----
AAAATGTGTATAAAGAAATAAATAGTTTTTCTTTC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACTGCTTTGTTTCCCATTTCTGCACCAATCCCTTGACAGTATAAAGAACT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 1 r 143562785 143751231 46 99.7 Perfect prediction
Features of the protein sequence
Description

Length: 2296 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_001146341 0 99.0 phosphodiestera...
Pan troglodytes
CAH72528 0 99.9 phosphodiestera...
Homo sapiens
Q5VU43 0 92.2 Myomegalin; Pho...
Homo sapiens
CAH72523 0 92.2 phosphodiestera...
Homo sapiens
CAD91152 0 91.6 hypothetical pr...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB007946 6e-127 97.9 KIAA0477
AB046853 6e-11 24.7 KIAA1633
AB040945 1.6e-06 22.5 KIAA1512
AB020673 2.5e-06 22.0 KIAA0866
AB007914 1.7e-05 23.5 KIAA0445
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR012943 137 199 PF07989 Spindle associated
IPR010630 1568 1638 PF06758 Protein of unknown function DUF1220
Experimental conditions
Primer_f
Primer_r
PCR conditions °C sec °C sec cycles

RH mapping information
Description

Chromosome No. 1
Experimental conditions
Panel name GeneBridge 4
Primer_f AAACACATTCTACCAGCACTG
Primer_r CTTATCTCGGGGAAATTGCAG
PCR product length 153 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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