KIAA0090

Product ID	ORK00012
Accession No	D42044
Description	ER membrane protein complex subunit 1, transcript variant 2
Clone name	eh00576
Vector information	The cDNA fragment was inserted at the XhoI-SacI site of the ... The cDNA fragment was inserted at the XhoI-SacI site of the pBC SK+ vector(Cm resistance; http://www.kazusa.or.jp/huge/vectorinfo/pbcskp_s.txt) with sequences including attB1 and attB2 sites (CTCGAGCAGC TGAAGCTTTC ACAAGTTTGT ACAAAAAAGC AGGCTCTTC-5' end of cDNA, GAGCTCTCAC CACTTTGTAC AAGAAAGCTG GGCGGCCGCT TTTTTTTTTT TTTTTTT-3' end of cDNA).
cDNA sequence	DNA sequence (5411 bp) Predicted protein sequence (991 aa)
HaloTag ORF Clone	FHC00012
Flexi ORF Clone	FXC00012
Source
Rouge ID	mKIAA0090 by Kazusa Mouse cDNA Project
Note	We replaced ha03523, former representative clones for KIAA0090 with eh00576. (2005/08/06)

Length: 5411 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2435 bp
Genome contig ID	gi89161185r_19316071
PolyA signal sequence (None)	+--------+--------+----*----+---- GACCAGCCTGGGCAACATAGCAAGACTCTATCTAC
Flanking genome sequence (99884 - 99835)	----+--------+--------+--------+--------+----* AAAAAATAAAAAAAAATTAGCCGGGCCTGGTGGCATGTGCCTGTGGTCCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	19415955	19450587	23	99.5	Perfect prediction

Length: 991 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAF84307	99.8	unnamed protein...	Homo sapiens
CAH56140	99.8	hypothetical pr...	Homo sapiens
XP_001091700	99.0	similar to CG29...	Macaca mulatta
CAH56165	99.4	hypothetical pr...	Homo sapiens
XP_001504430	96.2	similar to CG29...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR011678	717	990	PF07774	Protein of unknown function DUF1620

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	6	SRFWLWATLLIPAAAVYEDQVGK	28	SECONDARY	23
SOSUI2	2	956	DDYDYVLISSVLFGLVFATMIT	977	PRIMARY	22

Primer_f
Primer_r
PCR conditions	test

Panel name
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-