KIAA1123

Product ID	ORK04415
Accession No	AB032949
Description	calcium channel, voltage-dependent, T type, alpha 1G subunit
Clone name	fh01458
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5475 bp) Predicted protein sequence (1717 aa)
Source	Human fetal brain
Rouge ID	mKIAA1123 by Kazusa Mouse cDNA Project
Note	We replaced hj05096, former representative clones for KIAA1123 with fh01458. (2002/5/10)

Length: 5475 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	320 bp
Genome contig ID	gi51511734f_45908409
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- GTATATTCTATTTTATTAAATTAATTGAATCTAGT
Flanking genome sequence (151024 - 151073)	----+--------+--------+--------+--------+----* ATATGCGGGATGTACGACATTTTGTGACTGAAGAGACTTGTTTCCTTCTA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	17	f	46008409	46059431	28	100.0	Perfect prediction

Length: 1717 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAF37696	100.0	voltage-depende...	Homo sapiens
AAF37689	99.5	voltage-depende...	Homo sapiens
ABF69908	99.6	T calcium chann...	Homo sapiens
XP_865671	94.4	similar to volt...	Canis lupus fam...
XP_001252667	94.2	calcium channel...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB032946	4e-85	54.9	KIAA1120
AB037777	5.3e-11	30.6	KIAA1356

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR005445	212	225	PR01629	T-type voltage-dependent calcium channel alpha 1 subunit
	IPR002077	355	381	PR00167	Ca2+ channel
	IPR002077	918	930	PR00167	Ca2+ channel
	IPR005445	999	1014	PR01629	T-type voltage-dependent calcium channel alpha 1 subunit
HMMPfam	IPR005821	228	414	PF00520	Ion transport
	IPR005821	763	987	PF00520	Ion transport
	IPR005821	1078	1283	PF00520	Ion transport

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	193	SKYFGRGIMIAILVNTLSMGIE	214	SECONDARY	22
	2	227	EISNIVFTSLFALEMLLKLLVYG	249	SECONDARY	23
	3	256	NPYNIFDGVIVVISVWEIVGQQG	278	SECONDARY	23
	4	319	FCMLLMLFIFIFSILGMHLFGCK	341	PRIMARY	23
	5	356	NFDSLLWAIVTVFQILTQEDWNK	378	SECONDARY	23
	6	394	YFIALMTFGNYVLFNLLVAILVE	416	PRIMARY	23
	7	727	KMFDHVVLVIIFLNCITIAMER	748	PRIMARY	22
	8	763	LSNYIFTAVFLAEMTVKVVALGW	785	SECONDARY	23
	9	804	LVLISVIDILVSMVSDSGTKILG	826	PRIMARY	23
	10	865	NIVVICCAFFIIFGILGVQLFK	886	PRIMARY	22
	11	922	NLGQALMSLFVLASKDGWVDIMY	944	SECONDARY	23
	12	966	LYFISFLLIVAFFVLNMFVGVVV	988	PRIMARY	23
	13	1045	YLDLFITGVIGLNVVTMAMEHYQ	1067	SECONDARY	23
	14	1077	KICNYIFTVIFVLESVFKLVAFG	1099	SECONDARY	23
	15	1107	RWNQLDLAIVLLSIMGITLEEIE	1129	PRIMARY	23
	16	1178	NLGLLFMLLFFIFAALGVELFGD	1200	PRIMARY	23
	17	1259	ISPIYFVSFVLTAQFVLVNVVIA	1281	PRIMARY	23

Primer_f	TCTTCATCACAGGTGTCATCG
Primer_r	ATGATGGACAGCAGCACAATG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	GAGTTAAAAGCAGCAGCCCCG
Primer_r	TGTCGGGAACCTCAGCTGCAC
PCR product length	75 bp
PCR conditions	95 °C15 sec68 °C60 sec30 cycles