KIAA1220

Product ID	ORK05357
Accession No	AB033046
Description	glutamate receptor, ionotropic, delta 1
Clone name	fh03489
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5097 bp) Predicted protein sequence (791 aa)
Source	Human fetal brain

Length: 5097 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2719 bp
Genome contig ID	gi89161187r_87249292
PolyA signal sequence (ATTAAA,-19)	+--------+--------+----*----+---- AATAATCAGCATTTGGATTAAAGTTGTTTAATAAT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAATCTTGTCTTTGCTCCCTATGCTCTTATTCTCTGGGGTGAAGAAATAC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	10	r	87349292	87888629	12	99.4	Internal No-hit

Length: 791 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9ULK0	100.0	Glutamate recep...	Homo sapiens
BAF85793	99.9	unnamed protein...	Homo sapiens
XP_001085502	99.6	glutamate recep...	Macaca mulatta
Q62640	98.1	Glutamate recep...	Rattus norvegicus
XP_001917018	98.4	similar to Glut...	Equus caballus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB075853	9.6e-05	23.8	KIAA1973

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	409	474	PD245531	NULL
FPrintScan	IPR001508	262	290	PR00177	NMDA receptor
	IPR001508	347	372	PR00177	NMDA receptor
	IPR001508	414	441	PR00177	NMDA receptor
	IPR001508	613	637	PR00177	NMDA receptor
HMMPfam	IPR001828	1	183	PF01094	Extracellular ligand-binding receptor
	IPR001638	245	329	PF00497	Bacterial extracellular solute-binding protein
	IPR001320	344	634	PF00060	Ionotropic glutamate receptor
HMMSmart	IPR001320	220	589	SM00079	Ionotropic glutamate receptor
ScanRegExp	IPR002086	685	692	PS00687	Aldehyde dehydrogenase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	340	FAPFDFAVWACIAAAIPVVGVLI	362	PRIMARY	23
	2	382	SASATLHSAIWIVYGAFVQQGGE	404	SECONDARY	23
	3	413	RIVMGSWWLFTLIVCSSYTANLA	435	SECONDARY	23
	4	612	SFAGVFCILAIGLLLACLVAALE	634	PRIMARY	23

Primer_f	ATCAAACTCTGGTCTCACTGC
Primer_r	GAAGACATCCAAGCTATACCC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	ATCAAACTCTGGTCTCACTGC
Primer_r	GAAGACATCCAAGCTATACCC
PCR product length	97 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles