KIAA1337

Product ID	ORK00806
Accession No	AB037758
Description	patched domain containing 2
Clone name	fh16696
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5181 bp) Predicted protein sequence (1438 aa)
HaloTag ORF Clone	FHC00806
Flexi ORF Clone	FXC00806
Source	Human fetal brain
Rouge ID	mKIAA1337 by Kazusa Mouse cDNA Project

Length: 5181 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	864 bp
Genome contig ID	gi89161185f_11361882
PolyA signal sequence (ATTAAA,-21)	+--------+--------+----*----+---- GGCAGAAGGTTGCTATTAAAATGACATAGGATTGC
Flanking genome sequence (158314 - 158363)	----+--------+--------+--------+--------+----* AGACTGCTGCTGTTAATACGAGCATTTTAAGCACTGCTGTCTCTACCGCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	f	11461882	11520194	21	99.8	Perfect prediction

Length: 1438 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
CAH70606	99.9	patched domain ...	Homo sapiens
BAG10036	100.0	patched domain-...	synthetic construct
Q9P2K9	99.9	Patched domain-...	Homo sapiens
XP_001915151	92.7	patched domain ...	Equus caballus
XP_873073	91.1	similar to patc...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB051529	5.8e-05	31.2	KIAA1742

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003392	285	1392	PF02460	Patched
ProfileScan	IPR000731	556	661	PS50156	Sterol-sensing 5TM box

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	118	CCAGLVLFLGCSIPMALSAFMFL	140	PRIMARY	23
	2	506	NNDMLLAFISSSCIAALVYILTS	528	PRIMARY	23
	3	536	FGIASIGLSCLVALFLYHVVFGI	558	PRIMARY	23
	4	565	NGVAAFVIVGIGVDDVFVFINTY	587	PRIMARY	23
	5	611	TFFTSLTTAAAYAANVFSQIPAV	633	SECONDARY	23
	6	642	LIVSCCWLAVLVTMPAALGLWSL	664	PRIMARY	23
	7	775	RWVIVGLFVSILILSLVFASR	795	PRIMARY	21
	8	932	ACMSTVGLLQAASPSRKWMLTTL	954	SECONDARY	23
	9	1047	VRPLVDTGAMVFVVFGIIGVNR	1068	PRIMARY	22
	10	1220	FMEIVGVQSALCGLVLSLLICVA	1242	PRIMARY	23
	11	1251	ILLLLPVLLSILGIVCLVVTIMY	1273	PRIMARY	23
	12	1285	ISLSILVGSSVDYCVHLVEGYLL	1307	SECONDARY	23
	13	1334	HVGVAIVSSALTTVIATVPLFFC	1356	PRIMARY	23
	14	1370	LNTGVSILYTLTVSTALLGIMAP	1392	SECONDARY	23
	15	1403	LKALGAVLLAGALGLGACLVLLQ	1425	PRIMARY	23

Primer_f	TGTGGTCTTCGGCATTATTGG
Primer_r	AGGTCAAAGCTGCTGTCGTAG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	TGTGGTCTTCGGCATTATTGG
Primer_r	AGGTCAAAGCTGCTGTCGTAG
PCR product length	99(1.0k) bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles