KIAA0245

Product ID	ORK00475
Accession No	D87432
Description	solute carrier family 7 (amino acid transporter light chain, y+L system), member 6, transcript variant 2
Clone name	ha07016
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6296 bp) Predicted protein sequence (552 aa)
HaloTag ORF Clone	FHC00475
Flexi ORF Clone	FXC00475
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0245 by Kazusa Mouse cDNA Project

Length: 6296 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	4487 bp
Genome contig ID	gi51511732f_66757995
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- TGTTATCAGACACCAATAAATTCTTTTTGTTGGGC
Flanking genome sequence (135230 - 135279)	----+--------+--------+--------+--------+----* CTTCTTCTCTCTTCTTTAATATAGTTACTACGGGAATTATGTTGTCAGAG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	16	f	66857995	66893223	11	99.3	Terminal No-hit

Length: 552 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
XP_001146701	7.5e-211	98.3	solute carrier ...	Pan troglodytes
Q92536	2e-205	100.0	Y+L amino acid ...	Homo sapiens
CAH18146	2e-204	99.6	hypothetical pr...	Homo sapiens
NP_001069405	1.3e-186	89.9	solute carrier ...	Bos taurus
EDL92441	4e-186	89.5	solute carrier ...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB046833	4.5e-05	21.1	KIAA1613

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004841	86	519	PF00324	Amino acid permease-associated region

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	83	SLLNGVSLVVGNMIGSGIFVSPK	105	SECONDARY	23
	2	115	GMSLIVWAIGGLFSVVGALCYAE	137	PRIMARY	23
	3	159	FIAFIRLWVSLLVVEPTGQAIIA	181	PRIMARY	23
	4	201	LACRLLAAACICLLTFVNCAYVK	223	PRIMARY	23
	5	232	FTYAKVVALIAIIVMGLVKLCQG	254	PRIMARY	23
	6	313	PIVTLIYILTNVAYYTVLNISDV	335	PRIMARY	23
	7	357	IPIAVALSCFGGLNASIFASSRL	379	SECONDARY	23
	8	403	PIPALLFNCTMALIYLIVEDVFQ	425	PRIMARY	23
	9	460	KLSVFFPIVFCICSVFLVIVPLF	482	PRIMARY	23
	10	486	INSLIGIGIALSGVPFYFMGVY	507	PRIMARY	22

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	CCTTCACACTGGAGTATTTTG
Primer_r	TTGCATATTCTTTTGGGGAGG
PCR product length	170 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles