|
Order Kazusa clone(s) from : |
| Product ID | ORK00043 |
|---|---|
| Accession No | D87743 |
| Description | solute carrier family 9, subfamily A (NHE6, cation proton antiporter 6), member 6, transcript variant 2 |
| Clone name | ha07045 |
| Vector information | |
| cDNA sequence | DNA sequence (4408 bp) Predicted protein sequence (666 aa) |
|
HaloTag ORF Clone |
FHC00043
|
| Flexi ORF Clone | FXC00043 |
| Source | Myeloblast cell line (KG-1) |
| Rouge ID |
mKIAA0267
by Kazusa Mouse cDNA Project
|
Length: 4408 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 2407 bp |
|---|---|
| Genome contig ID | gi89161218f_134795337 |
| PolyA signal sequence (ATTAAA,-34) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (161623 - 161672) |
----+----*----+----*----+----*----+----*----+----* |
Length: 666 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR002090 | 3 | 21 | PR01088 | Na+/H+ exchanger |
| IPR002090 | 22 | 40 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 41 | 60 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 61 | 85 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 86 | 104 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 105 | 131 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 133 | 146 | PR01088 | Na+/H+ exchanger | |
| IPR004709 | 147 | 158 | PR01084 | Sodium/hydrogen exchanger subfamily | |
| IPR004709 | 161 | 175 | PR01084 | Sodium/hydrogen exchanger subfamily | |
| IPR004709 | 176 | 184 | PR01084 | Sodium/hydrogen exchanger subfamily | |
| IPR004709 | 221 | 231 | PR01084 | Sodium/hydrogen exchanger subfamily | |
| IPR002090 | 264 | 281 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 283 | 302 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 494 | 520 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 521 | 539 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 545 | 573 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 574 | 601 | PR01088 | Na+/H+ exchanger | |
| IPR002090 | 606 | 627 | PR01088 | Na+/H+ exchanger | |
| HMMPfam | IPR006153 | 75 | 500 | PF00999 | Sodium/hydrogen exchanger |
| HMMTigr | IPR004709 | 61 | 652 | TIGR00840 | Sodium/hydrogen exchanger subfamily |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 21 | LMRPLWLLLAVGVFDWAGASD | 41 | PRIMARY | 21 | 2 | 70 | NLLIFILLLTLTILTIWLFK | 89 | PRIMARY | 20 | 3 | 97 | HETGLAMIYGLLVGLVLRYGIHV | 119 | PRIMARY | 23 | 4 | 139 | LLVTFDPEVFFNILLPPIIFYAG | 161 | SECONDARY | 23 | 5 | 176 | ILAYAFLGTAISCFVIGSIMYGC | 198 | PRIMARY | 23 | 6 | 216 | DCLLFGAIVSATDPVTVLAIFHE | 238 | SECONDARY | 23 | 7 | 247 | ALLFGESVLNDAVAIVLSSSIVA | 269 | SECONDARY | 23 | 8 | 288 | SIGIFLGIFSGSFAMGAATGVVT | 310 | SECONDARY | 23 | 9 | 331 | FLMSWSTFLLAEAWGFTGVVAVL | 353 | PRIMARY | 23 | 10 | 412 | VGAFVAIFLGRAANIYPLSLLLN | 434 | SECONDARY | 23 | 11 | 480 | LLIVFFTVWVFGGGTTAMLSCLH | 502 | PRIMARY | 23 |
|---|
Chromosome No. X
Experimental conditions| Panel name | Genebridge 4 |
|---|---|
| Primer_f | CATTTATCCTAGTTGTCCGTG |
| Primer_r | CCACTATAACATCTGTCCAAG |
| PCR product length | 108 bp |
| PCR conditions | 95 °C 15 sec 60 °C 60 sec 30 cycles |