Gene/Protein Characteristic Table for KIAA0267
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Product ID ORK00043
Accession No D87743
Description solute carrier family 9, subfamily A (NHE6, cation proton antiporter 6), member 6, transcript variant 2
Clone name ha07045
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (4408 bp)
Predicted protein sequence (666 aa)
Flexi ORF Clone FXC00043
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0267 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4408 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 2407 bp
Genome contig ID gi89161218f_134795337
PolyA signal sequence
(ATTAAA,-34)
+----*----+----*----+----*----+----
CATTAAAAAAGTTATAGCCTTGCAAATAACCACTC
Flanking genome sequence
(161623 - 161672)
----+----*----+----*----+----*----+----*----+----*
ACAGTATTTGAGTCACAGTTTATTTTAGTGAGACGTAAAGAGTGCTGACT
Features of the protein sequence
Description

Length: 666 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q92581 0 100.0 Sodium/hydrogen...
Homo sapiens
EAW88482 0 99.7 solute carrier ...
Homo sapiens
XP_001101621 0 99.4 similar to solu...
Macaca mulatta
BAG11159 0 100.0 solute carrier ...
synthetic construct
CAQ12099 0 96.4 solute carrier ...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB023156 5.9e-53 36.1 KIAA0939
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
FPrintScan IPR002090 3 21 PR01088 Na+/H+ exchanger
IPR002090 22 40 PR01088 Na+/H+ exchanger
IPR002090 41 60 PR01088 Na+/H+ exchanger
IPR002090 61 85 PR01088 Na+/H+ exchanger
IPR002090 86 104 PR01088 Na+/H+ exchanger
IPR002090 105 131 PR01088 Na+/H+ exchanger
IPR002090 133 146 PR01088 Na+/H+ exchanger
IPR004709 147 158 PR01084 Sodium/hydrogen exchanger subfamily
IPR004709 161 175 PR01084 Sodium/hydrogen exchanger subfamily
IPR004709 176 184 PR01084 Sodium/hydrogen exchanger subfamily
IPR004709 221 231 PR01084 Sodium/hydrogen exchanger subfamily
IPR002090 264 281 PR01088 Na+/H+ exchanger
IPR002090 283 302 PR01088 Na+/H+ exchanger
IPR002090 494 520 PR01088 Na+/H+ exchanger
IPR002090 521 539 PR01088 Na+/H+ exchanger
IPR002090 545 573 PR01088 Na+/H+ exchanger
IPR002090 574 601 PR01088 Na+/H+ exchanger
IPR002090 606 627 PR01088 Na+/H+ exchanger
HMMPfam IPR006153 75 500 PF00999 Sodium/hydrogen exchanger
HMMTigr IPR004709 61 652 TIGR00840 Sodium/hydrogen exchanger subfamily

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 21 LMRPLWLLLAVGVFDWAGASD 41 PRIMARY 21
2 70 NLLIFILLLTLTILTIWLFK 89 PRIMARY 20
3 97 HETGLAMIYGLLVGLVLRYGIHV 119 PRIMARY 23
4 139 LLVTFDPEVFFNILLPPIIFYAG 161 SECONDARY 23
5 176 ILAYAFLGTAISCFVIGSIMYGC 198 PRIMARY 23
6 216 DCLLFGAIVSATDPVTVLAIFHE 238 SECONDARY 23
7 247 ALLFGESVLNDAVAIVLSSSIVA 269 SECONDARY 23
8 288 SIGIFLGIFSGSFAMGAATGVVT 310 SECONDARY 23
9 331 FLMSWSTFLLAEAWGFTGVVAVL 353 PRIMARY 23
10 412 VGAFVAIFLGRAANIYPLSLLLN 434 SECONDARY 23
11 480 LLIVFFTVWVFGGGTTAMLSCLH 502 PRIMARY 23
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. X
Experimental conditions
Panel name Genebridge 4
Primer_f CATTTATCCTAGTTGTCCGTG
Primer_r CCACTATAACATCTGTCCAAG
PCR product length 108 bp
PCR conditions 95 °C15 sec60 °C60 sec30 cycles
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