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Order Kazusa clone(s) from : |
| Product ID | ORK00572 |
|---|---|
| Accession No | AB011176 |
| Description | endothelin converting enzyme 2, transcript variant 5 |
| Clone name | hg02198b |
| Vector information | |
| cDNA sequence | DNA sequence (3228 bp) Predicted protein sequence (773 aa) |
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HaloTag ORF Clone |
FHC00572
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| Flexi ORF Clone | FXC00572 |
| Source | Human adult brain |
Length: 3228 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 773 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR000718 | 561 | 573 | PR00786 | Peptidase M13 |
| IPR000718 | 579 | 591 | PR00786 | Peptidase M13 | |
| IPR000718 | 600 | 616 | PR00786 | Peptidase M13 | |
| IPR000718 | 670 | 681 | PR00786 | Peptidase M13 | |
| HMMPfam | IPR008753 | 123 | 510 | PF05649 | Peptidase M13 |
| IPR000718 | 569 | 772 | PF01431 | Peptidase M13 | |
| ScanRegExp | IPR006025 | 607 | 616 | PS00142 | Peptidase M |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 68 | ELVLAGASLLLAALLLGCLVALG | 90 | PRIMARY | 23 |
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RT-PCR
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Experimental conditions| Primer_f | GGGGAAGTGCATATGTGTAGC |
|---|---|
| Primer_r | CCTTTTCCCTGCTCTATCTGC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 3
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | GGGGAAGTGCATATGTGTAGC |
| Primer_r | CCTTTTCCCTGCTCTATCTGC |
| PCR product length | 113 bp |
| PCR conditions | 95 °C 15 sec 64 °C 60 sec 30 cycles |