KIAA0800

Product ID	ORK01675
Accession No	AB018343
Description	Vpr (HIV-1) binding protein, transcript variant 1
Clone name	hg03850s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pYO326GW (Amp-r) vector. The adjacent sequence to the 5' end of the cDNA is as follows: TCGACCCACG CGTCCGCGGA CGCGTGGG-5' end of cDNA. The 3'end of the cDNA was inserted at the NotI site of the vector. The original pYO326 shuttle vector (Amp-r) was kindly provided by Y. Ohya (University of Tokyo).
cDNA sequence	DNA sequence (5984 bp) Predicted protein sequence (1521 aa)
HaloTag ORF Clone	FHC01675
Flexi ORF Clone	FXC01675
Source	Human adult brain
Rouge ID	mKIAA0800 by Kazusa Mouse cDNA Project
Note	We replaced hg03850, former representative clones for KIAA0800 with hg03850s1. (2002/5/10)

Length: 5984 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1292 bp
Genome contig ID	gi89161205r_51308382
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- AAAACAGGCAAATAAATAAACATGCTTAACATGTC
Flanking genome sequence (99956 - 99907)	----+--------+--------+--------+--------+----* TTTGTGTTGCTGTTTTGTTATGAACGTGCTTGCTCTCTTGCGAGGCACCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	3	r	51408338	51509041	25	100.0	Internal No-hit

Length: 1521 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9Y4B6	100.0	Protein VPRBP; ...	Homo sapiens
EAW65142	99.9	Vpr (HIV-1) bin...	Homo sapiens
XP_001169544	99.9	VprBP protein i...	Pan troglodytes
AAI10372	99.9	VPRBP protein [...	Homo sapiens
EAW65141	99.5	Vpr (HIV-1) bin...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR013720	862	888	PF08513	LisH dimerisation motif
HMMSmart	IPR006594	860	892	SM00667	LisH dimerisation motif
ProfileScan	IPR006594	860	892	PS50896	LisH dimerisation motif

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	601	PAEVFLKLSCVQLLLQLISIACN	623	SECONDARY	23
SOSUI2	2	632	DTVRFALDVLAILTVVPKIQLQL	654	PRIMARY	23

Primer_f	AACAACATACCAAGGCAGCTC
Primer_r	AGTCAGTTGGGTACAGCAGGA
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	AACAACATACCAAGGCAGCTC
Primer_r	AGTCAGTTGGGTACAGCAGGA
PCR product length	160 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles