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Gene/Protein Characteristic Table for KIAA0366

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Product ID	ORK01075
Accession No	AB002364
Description	ADAM metallopeptidase with thrombospondin type 1 motif, 3
Clone name	hh00124
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5774 bp) Predicted protein sequence (1201 aa)
HaloTag ORF Clone	FHC01075
Flexi ORF Clone	FXC01075
Source	Human adult brain

Length: 5774 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 1201 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR013273	556	574	PR01857	Peptidase M12B
	IPR013273	669	688	PR01857	Peptidase M12B
	IPR013273	689	708	PR01857	Peptidase M12B
HMMPfam	IPR002870	3	197	PF01562	Peptidase M12B
	IPR001590	254	456	PF01421	Peptidase M12B
	IPR000884	551	601	PF00090	Thrombospondin
	IPR010294	709	823	PF05986	ADAM-TS Spacer 1
	IPR000884	843	900	PF00090	Thrombospondin
	IPR000884	905	962	PF00090	Thrombospondin
	IPR000884	966	1011	PF00090	Thrombospondin
HMMSmart	IPR000884	550	602	SM00209	Thrombospondin
	IPR000884	844	901	SM00209	Thrombospondin
	IPR000884	904	963	SM00209	Thrombospondin
	IPR000884	965	1012	SM00209	Thrombospondin
ProfileScan	IPR001590	252	456	PS50215	Peptidase M12B
	IPR000884	547	602	PS50092	Thrombospondin
	IPR000884	841	901	PS50092	Thrombospondin
	IPR000884	902	961	PS50092	Thrombospondin
	IPR000884	962	1012	PS50092	Thrombospondin
	IPR010909	1007	1050	PS50900	PLAC

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	291	ESLGVHINVVLVRMIMLGYAKSI	313	PRIMARY	23

RT-PCR

Experimental conditions

Chromosome No. 4
Experimental conditions

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