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Order Kazusa clone(s) from : |
| Product ID | ORK00530 |
|---|---|
| Accession No | AB007874 |
| Description | zinc finger and BTB domain containing 43, transcript variant 1 |
| Clone name | hh00161 |
| Vector information | |
| cDNA sequence | DNA sequence (5725 bp) Predicted protein sequence (492 aa) |
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HaloTag ORF Clone |
FHC00530
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| Flexi ORF Clone | FXC00530 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0414
by Kazusa Mouse cDNA Project
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Length: 5725 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 492 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| BlastProDom | IPR007087 | 425 | 448 | PD000003 | Zinc finger |
| HMMPfam | IPR013069 | 48 | 152 | PF00651 | BTB/POZ |
| IPR007087 | 398 | 419 | PF00096 | Zinc finger | |
| IPR007087 | 425 | 447 | PF00096 | Zinc finger | |
| IPR007087 | 453 | 475 | PF00096 | Zinc finger | |
| HMMSmart | IPR000210 | 58 | 152 | SM00225 | BTB/POZ-like |
| IPR015880 | 398 | 419 | SM00355 | Zinc finger | |
| IPR015880 | 425 | 447 | SM00355 | Zinc finger | |
| IPR015880 | 453 | 473 | SM00355 | Zinc finger | |
| ProfileScan | IPR000210 | 58 | 122 | PS50097 | BTB/POZ-like |
| IPR007087 | 398 | 424 | PS50157 | Zinc finger | |
| IPR007087 | 425 | 452 | PS50157 | Zinc finger | |
| IPR007087 | 453 | 482 | PS50157 | Zinc finger | |
| ScanRegExp | IPR007087 | 427 | 447 | PS00028 | Zinc finger |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 4 | IFGLEFVLIFLSLVAPNKICDEM | 26 | PRIMARY | 23 |
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RT-PCR
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Experimental conditions| Primer_f | CATGGGGATTGAAGAAAAACC |
|---|---|
| Primer_r | CACGTCAATGGCCTACAAACC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 9
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | CATGGGGATTGAAGAAAAACC |
| Primer_r | CACGTCAATGGCCTACAAACC |
| PCR product length | 102 bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |