KIAA1060

Product ID	ORK04050
Accession No	AB028983
Description	adenylate cyclase 2 (brain)
Clone name	hh13580
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5873 bp) Predicted protein sequence (887 aa)
Source	Human adult brain
Rouge ID	mKIAA1060 by Kazusa Mouse cDNA Project

Length: 5873 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3209 bp
Genome contig ID	gi51511721f_7579322
PolyA signal sequence (None)	+--------+--------+----*----+---- TCCAGCCTGGGTGACAGAGCGAGACTCTGTCTCAG
Flanking genome sequence (303874 - 303923)	----+--------+--------+--------+--------+----* AAAAAAAAAGAAAAAAAAGAAAAAGCAACATAATTGCCCAGTGATGAAAA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	5	f	7679322	7883194	22	99.4	Perfect prediction

Length: 887 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG64952	100.0	unnamed protein...	Homo sapiens
EAX08097	100.0	adenylate cycla...	Homo sapiens
AAI11744	100.0	Adenylate cycla...	Homo sapiens
Q08462	100.0	Adenylate cycla...	Homo sapiens
AAP97285	100.0	adenylyl cyclas...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
D25538	1.8e-197	58.7	KIAA0037
AB011083	3.4e-80	37.3	KIAA0511
AB007882	9.9e-48	42.4	KIAA0422
AB011092	6.2e-30	32.1	KIAA0520

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001054	77	261	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
	IPR009398	273	396	PF06327	Adenylate cyclase-like
	IPR001054	674	874	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMSmart	IPR001054	31	239	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMSmart	IPR001054	643	857	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
ProfileScan	IPR001054	86	213	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
ProfileScan	IPR001054	683	828	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
ScanRegExp	IPR001054	190	213	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase
ScanRegExp	IPR001054	805	828	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	399	YVTCACLIFFCIFIVQILVLPKT	421	PRIMARY	23
	2	427	SFGAAFLLLAFILFVCFAGQLLQ	449	PRIMARY	23
	3	475	RISLTIITTAIILMMAVFNMFFL	497	PRIMARY	23
	4	531	FFLPYFIYSCILGLISCSVFLRV	553	PRIMARY	23
	5	598	LKTMGSVSLSIFFITLLVLGRQ	619	SECONDARY	22

Primer_f	CGCTAGTGATTAAACGAGGCA
Primer_r	GTCATGCTCTACTCACACTTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	UniGene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-