KIAA0037

Product ID	ORK00380
Accession No	D25538
Description	adenylate cyclase 7, transcript variant 1
Clone name	ha00929
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6196 bp) Predicted protein sequence (1088 aa)
HaloTag ORF Clone	FHC00380
Flexi ORF Clone	FXC00380
Source	Myeloblast cell line (KG-1)

Length: 6196 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2688 bp
Genome contig ID	gi51511732f_48779327
PolyA signal sequence (ATTAAA,-25)	+--------+--------+----*----+---- CTTTTTATACATTAAATTAGCAATTTGAAAAACTC
Flanking genome sequence (130219 - 130268)	----+--------+--------+--------+--------+----* AAATATCTGAAGGTATTTTATTTTCGTGGTCCTCATGAACTCACTAGAGA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	16	f	48879327	48909544	26	100.0	Internal No-hit

Length: 1088 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
P51828	100.0	Adenylate cycla...	Homo sapiens
CAA89894	85.9	adenylyl cyclas...	Bos taurus
S68685	85.3	65 kda antigen ...	Mycobacterium g...
Q29450	85.2	Adenylate cycla...	Bos taurus
XP_001056453	84.2	similar to Aden...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB028983	5.3e-168	58.7	KIAA1060
AB011083	8.7e-70	37.4	KIAA0511
AB007882	6.8e-40	39.5	KIAA0422
AB011092	3.1e-27	30.4	KIAA0520

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001054	278	462	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
	IPR009398	476	601	PF06327	Adenylate cyclase-like
	IPR001054	878	1077	PF00211	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMSmart	IPR001054	232	440	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
HMMSmart	IPR001054	848	1060	SM00044	Adenylyl cyclase class-3/4/guanylyl cyclase
ProfileScan	IPR001054	287	414	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
ProfileScan	IPR001054	887	1031	PS50125	Adenylyl cyclase class-3/4/guanylyl cyclase
ScanRegExp	IPR001054	1008	1031	PS00452	Adenylyl cyclase class-3/4/guanylyl cyclase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	40	GPLLLTLLLVAATACVALIIIAF	62	PRIMARY	23
	2	73	ILGMAFLVLAVFAALSVLMYVEC	95	PRIMARY	23
	3	101	LRALALLTWACLVALGYVLVFDA	123	PRIMARY	23
	4	143	VVYTLLPFSMRGAVAVGAVSTAS	165	PRIMARY	23
	5	182	VRVGLQLLANAVIFLCGNLTGAF	204	SECONDARY	23
	6	603	HDFACASLIFVCILLVHVLLMPR	625	PRIMARY	23
	7	630	GVSFGLVACVLGLVLGLCFATKF	652	PRIMARY	23
	8	673	PLLRLTLAVLTIGSLLTVAIINL	695	SECONDARY	23
	9	740	ACSVFLRMSLEPKVVLLTVALVA	762	PRIMARY	23
	10	804	KTMTNFYLVLFYITLLTLSRQID	826	SECONDARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Stanford G3
Primer_f	ATGACCAAACTGCCCGTGACT
Primer_r	ATTTTCGTTCCCTCCACCGCC
PCR product length	317 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles