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Order Kazusa clone(s) from : |
| Product ID | ORK00569 |
|---|---|
| Accession No | AB011160 |
| Description | protocadherin gamma subfamily A, 12, transcript variant 1 |
| Clone name | hj02685 |
| Vector information | |
| cDNA sequence | DNA sequence (4747 bp) Predicted protein sequence (938 aa) |
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HaloTag ORF Clone |
FHC00569
|
| Flexi ORF Clone | FXC00569 |
| Source | Human adult brain |
Length: 4747 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Length: 938 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| FPrintScan | IPR002126 | 79 | 98 | PR00205 | Cadherin |
| IPR002126 | 248 | 277 | PR00205 | Cadherin | |
| IPR002126 | 320 | 332 | PR00205 | Cadherin | |
| IPR002126 | 332 | 351 | PR00205 | Cadherin | |
| IPR002126 | 456 | 469 | PR00205 | Cadherin | |
| IPR002126 | 516 | 542 | PR00205 | Cadherin | |
| IPR002126 | 550 | 567 | PR00205 | Cadherin | |
| HMMPfam | IPR013164 | 35 | 118 | PF08266 | Cadherin |
| IPR002126 | 144 | 239 | PF00028 | Cadherin | |
| IPR002126 | 253 | 344 | PF00028 | Cadherin | |
| IPR002126 | 358 | 449 | PF00028 | Cadherin | |
| IPR002126 | 463 | 559 | PF00028 | Cadherin | |
| IPR002126 | 588 | 671 | PF00028 | Cadherin | |
| HMMSmart | IPR002126 | 51 | 137 | SM00112 | Cadherin |
| IPR002126 | 161 | 246 | SM00112 | Cadherin | |
| IPR002126 | 270 | 351 | SM00112 | Cadherin | |
| IPR002126 | 375 | 456 | SM00112 | Cadherin | |
| IPR002126 | 480 | 566 | SM00112 | Cadherin | |
| IPR002126 | 597 | 675 | SM00112 | Cadherin | |
| ProfileScan | IPR002126 | 81 | 139 | PS50268 | Cadherin |
| IPR002126 | 140 | 248 | PS50268 | Cadherin | |
| IPR002126 | 249 | 353 | PS50268 | Cadherin | |
| IPR002126 | 354 | 458 | PS50268 | Cadherin | |
| IPR002126 | 459 | 568 | PS50268 | Cadherin | |
| IPR002126 | 585 | 689 | PS50268 | Cadherin | |
| ScanRegExp | IPR002126 | 127 | 137 | PS00232 | Cadherin |
| IPR002126 | 236 | 246 | PS00232 | Cadherin | |
| IPR002126 | 341 | 351 | PS00232 | Cadherin | |
| IPR002126 | 446 | 456 | PS00232 | Cadherin | |
| IPR002126 | 556 | 566 | PS00232 | Cadherin |
Prediction of transmembrane (TM) segments| Method | No. | N terminal | transmembrane region | C terminal | type | length | SOSUI2 | 1 | 13 | HRDYKGLVLLGILLGTLWETGCT | 35 | SECONDARY | 23 | 2 | 697 | LYLVVAVAAVSCVFLAFVILLLA | 719 | PRIMARY | 23 |
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RT-PCR
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Experimental conditions| Primer_f | CCTCCTTGTGCATAGACCTTC |
|---|---|
| Primer_r | GCACACCGCACTACACTACTG |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 5
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | CCTCCTTGTGCATAGACCTTC |
| Primer_r | GCACACCGCACTACACTACTG |
| PCR product length | 161 bp |
| PCR conditions | 95 °C 15 sec 66 °C 60 sec 30 cycles |