KIAA0634

Product ID	ORK00582
Accession No	AB014534
Description	astrotactin 2, transcript variant 1
Clone name	hj03064
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4591 bp) Predicted protein sequence (1321 aa)
HaloTag ORF Clone	FHC00582
Flexi ORF Clone	FXC00582
Source	Human adult brain
Rouge ID	mKIAA0634 by Kazusa Mouse cDNA Project

Length: 4591 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	623 bp
Genome contig ID	gi89161216r_118127328
PolyA signal sequence (ATTAAA,-23)	+--------+--------+----*----+---- GCCTGTCCATGCATTAAAAATTCCTATTGTCTCTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TTTCTGCTTGGGTGGTATTTCTTACCAAGGGAATGTGCTTGAGAGACCTC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	9	r	118227328	119217138	22	99.7	Perfect prediction

Length: 1321 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
CAH72264	100.0	astrotactin 2 [...	Homo sapiens
NP_062387	97.0	astrotactin 2 i...	Mus musculus
O75129	96.2	Astrotactin-2; ...	Homo sapiens
XP_528403	94.6	astrotactin 2 [...	Pan troglodytes
XP_001098403	95.4	similar to astr...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB006627	6.5e-142	48.9	KIAA0289

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR003961	1048	1164	PF00041	Fibronectin
HMMSmart	IPR001862	833	1017	SM00457	Membrane attack complex component/perforin/complement C9

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	2	ASASAVSAAASSSSFATAATAAA	24	SECONDARY	23
	2	236	HILHISVMGGLIALLLLLLVFTV	258	PRIMARY	23
	3	415	NKTALTLIAVSSCILAMVCGSQ	436	PRIMARY	22

Primer_f	CTATGGAGGGACAGTTTTGAC
Primer_r	GGTCCCACAAACTCAATAATG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CTATGGAGGGACAGTTTTGAC
Primer_r	GGTCCCACAAACTCAATAATG
PCR product length	155 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles