KIAA1062

Product ID	ORK04006
Accession No	AB028985
Description	ATP-binding cassette, sub-family A (ABC1), member 2
Clone name	hj03579s1
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6011 bp) Predicted protein sequence (1771 aa)
Source	Human adult brain
Rouge ID	mKIAA1062 by Kazusa Mouse cDNA Project
Note	We replaced hj03579, former representative clones for KIAA1062 with hj03579s1. (2002/5/10)

Length: 6011 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	695 bp
Genome contig ID	gi89161216r_138921507
PolyA signal sequence (AATAAA,-15)	+--------+--------+----*----+---- ACAGATGTTTGTTTTAAATAAATAAACAAAATGTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TGCGGGTCTGCGTGTGTGGGTTGGGAAGGGTGGGGAGGATGGGCGCATCA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	9	r	139021507	139032346	35	99.8	Perfect prediction

Length: 1771 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9BZC7	100.0	ATP-binding cas...	Homo sapiens
AAG09372	100.0	ATP-binding cas...	Homo sapiens
NP_001597	100.0	ATP-binding cas...	Homo sapiens
NP_997698	100.0	ATP-binding cas...	Homo sapiens
BAG10412	99.9	ATP-binding cas...	synthetic construct

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB067475	1.5e-25	28.9	KIAA1888
AB020629	2.8e-07	25.9	KIAA0822

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR003439	458	501	PD000006	ABC transporter related
BlastProDom	IPR003439	1521	1564	PD000006	ABC transporter related
HMMPfam	IPR003439	353	533	PF00005	ABC transporter related
HMMPfam	IPR003439	1416	1597	PF00005	ABC transporter related
HMMSmart	IPR003593	352	534	SM00382	AAA+ ATPase
HMMSmart	IPR003593	1415	1600	SM00382	AAA+ ATPase
ProfileScan	IPR003439	326	557	PS50893	ABC transporter related
ProfileScan	IPR003439	1386	1621	PS50893	ABC transporter related
ScanRegExp	IPR003439	459	473	PS00211	ABC transporter related
	IPR002345	759	772	PS00213	Lipocalin
	IPR013032	976	987	PS00022	EGF-like region

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	37	FVIEHMMPLCMVISWVYSVAMTI	59	SECONDARY	23
	2	87	AWFITGFVQLSISVTALTAILKY	109	PRIMARY	23
	3	120	IIWLFLAVYAVATIMFCFLVSVL	142	PRIMARY	23
	4	148	LASACGGIIYFLSYVPYMYVAIR	170	PRIMARY	23
	5	183	KCIASLMSTTAFGLGSKYFALYE	205	SECONDARY	23
	6	226	FNLLLAVTMLMVDAVVYGILTWY	248	PRIMARY	23
	7	1123	LQGTDVVIAIFIIVAMSFVPASF	1145	PRIMARY	23
	8	1172	WLANYVWDMLNYLVPATCCVIIL	1194	PRIMARY	23
	9	1209	PAVLSLFLLYGWSITPIMYPASF	1231	PRIMARY	23
	10	1240	YVFLIVINLFIGITATVATFLLQ	1262	PRIMARY	23
	11	1270	LKVVNSYLKSCFLIFPNYNLGHG	1292	SECONDARY	23
	12	1323	IVTRGLVAMAVEGVVGFLLTIMC	1345	SECONDARY	23

Primer_f	CAGGGACTCAACAATGGGGAC
Primer_r	AAAGCAGGGCAAGGGTGTACG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	CAGGGACTCAACAATGGGGAC
Primer_r	AAAGCAGGGCAAGGGTGTACG
PCR product length	188 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles