KIAA0830

Product ID	ORK00135
Accession No	AB020637
Description	endonuclease domain containing 1
Clone name	hj04436
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4638 bp) Predicted protein sequence (523 aa)
HaloTag ORF Clone	FHC00135
Flexi ORF Clone	FXC00135
Source	Human adult brain
Rouge ID	mKIAA0830 by Kazusa Mouse cDNA Project

Length: 4638 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3066 bp
Genome contig ID	gi51511727f_94362671
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- GCACCTTGCAGATGCTCAATAAAGTAATTACTGAC
Flanking genome sequence (142786 - 142835)	----+--------+--------+--------+--------+----* AACTCGTTAGCAGTTCTGTAAAATATCTAAAAGGGGAGAATCCTAATAGT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	11	f	94462671	94505455	2	99.1	Perfect prediction

Length: 523 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
O94919	1.4e-208	100.0	Endonuclease do...	Homo sapiens
BAF84362	1.5e-207	99.6	unnamed protein...	Homo sapiens
XP_522156	3.8e-207	99.4	endonuclease do...	Pan troglodytes
XP_001090534	1.4e-203	97.0	endonuclease do...	Macaca mulatta
AAI51614	3.9e-164	76.6	ENDOD1 protein ...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001604	84	289	PF01223	DNA/RNA non-specific endonuclease
HMMSmart	IPR001604	85	290	SM00477	DNA/RNA non-specific endonuclease

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	23	AMGTARWLALGSLFALAGLLEGR	45	SECONDARY	23
	2	235	KVAVPEFVWLAACCAVPGGGWAM	257	PRIMARY	23
	3	373	VVAILKNIVYFLWCVTKQVINGI	395	PRIMARY	23
	4	424	VLKVVAKVIRALLRILCCLLKAI	446	PRIMARY	23
	5	479	ALGLGGTVSLLFDTAFGTLGGLF	501	SECONDARY	23

Primer_f	CCTGTTCCTAATGAGCTACGC
Primer_r	GGTTCACTTTGGTTCCTCCTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	UniGene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-