FLJ00135

Product ID	ORK05091
Accession No	AK074064
Clone name	sh02493
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5448 bp) Predicted protein sequence (597 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Length: 597 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAB84890	100.0	FLJ00135 protei...	Homo sapiens
AAH29271	100.0	PIGO protein [H...	Homo sapiens
Q8TEQ8	100.0	GPI ethanolamin...	Homo sapiens
XP_001165467	99.7	phosphatidylino...	Pan troglodytes
CAD38806	99.7	hypothetical pr...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AK090433	1.4e-214	99.6	FLJ00350

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	82	RMAGGTALLAASCFICLLASQWA	104	PRIMARY	23
	2	108	GFPFCPLLLTPVAWGLVGAIAYA	130	PRIMARY	23
	3	135	TIELKLDLVLLGAVAAVSSFLPF	157	PRIMARY	23
	4	173	TLFPIPGPVLLLLLFRLAVFFSD	195	PRIMARY	23
	5	203	RATPFLLGSFILLLVVQLHWEGQ	225	PRIMARY	23
	6	251	AYALRLGIGLLLCTRLAGLFHRC	273	SECONDARY	23
	7	299	NLWYGACVAALVALLAAVRLWL	320	PRIMARY	22
	8	332	PMLFVRWGLPLMALGTAAYWALA	354	SECONDARY	23
	9	372	SMVLPRAVAGLAASGLALLLWKP	394	SECONDARY	23
	10	456	AAYQLGSVYSAAMVTALTLLAFP	478	PRIMARY	23
	11	489	LVFLLLFLQSFLLLHLLAAGIPV	511	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test