FLJ00365

Product ID	ORK05863
Accession No	AK090444
Clone name	sh05134
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5484 bp) Predicted protein sequence (399 aa)
Source	Human spleen
Rouge ID	mFLJ00365 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Length: 399 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAC03425	6.4e-171	100.0	FLJ00365 protei...	Homo sapiens
EAX08154	7.4e-171	100.0	acyltransferase...	Homo sapiens
Q8NF37	8e-171	100.0	1-acylglyceroph...	Homo sapiens
AAH20166	3.6e-169	100.0	LPCAT1 protein ...	Homo sapiens
XP_517613	4e-167	97.8	acyltransferase...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR002048	249	309	PD000012	Calcium-binding EF-hand
HMMPfam	IPR002123	1	102	PF01553	Phospholipid/glycerol acyltransferase
	IPR002048	248	276	PF00036	Calcium-binding EF-hand
	IPR002048	285	313	PF00036	Calcium-binding EF-hand
	IPR002048	320	348	PF00036	Calcium-binding EF-hand
HMMSmart	IPR002048	248	276	SM00054	Calcium-binding EF-hand
HMMSmart	IPR002048	320	348	SM00054	Calcium-binding EF-hand
ProfileScan	IPR002123	1	152	PS50239	Phospholipid/glycerol acyltransferase
ProfileScan	IPR002048	284	345	PS50222	Calcium-binding EF-hand

Primer_f
Primer_r
PCR conditions	test