FLJ00156

Product ID	ORK05099
Accession No	AK074085
Clone name	sh06477
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (5860 bp) Predicted protein sequence (1887 aa)
HaloTag ORF Clone	FHC05099
Flexi ORF Clone	FXC05099
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 1887 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAB84911	99.9	FLJ00156 protei...	Homo sapiens
EAW93118	98.6	hCG1745555, iso...	Homo sapiens
Q6ZS81	97.5	Protein WDFY4.	Homo sapiens
XP_001926773	85.8	similar to WD r...	Sus scrofa
XP_606192	85.7	similar to hCG1...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB046827	0	98.0	KIAA1607
AK024502	4.4e-98	100.0	FLJ00111
AB023210	3.3e-70	38.3	KIAA0993
AB011112	3.4e-57	31.1	KIAA0540
AK131104	4.1e-57	31.1	FLJ00341

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	NULL	1265	1427	PD007848	NULL
FPrintScan	IPR001680	1659	1673	PR00320	WD-40 repeat
	IPR001680	1708	1722	PR00320	WD-40 repeat
	IPR001680	1798	1812	PR00320	WD-40 repeat
HMMPfam	IPR000409	1250	1532	PF02138	Beige/BEACH
	IPR001680	1684	1721	PF00400	WD-40 repeat
	IPR001680	1769	1811	PF00400	WD-40 repeat
HMMSmart	IPR001680	1632	1672	SM00320	WD-40 repeat
	IPR001680	1682	1721	SM00320	WD-40 repeat
	IPR001680	1724	1762	SM00320	WD-40 repeat
	IPR001680	1764	1811	SM00320	WD-40 repeat
ProfileScan	IPR000409	1257	1532	PS50197	Beige/BEACH
	IPR001680	1689	1730	PS50082	WD-40 repeat
	IPR001680	1689	1820	PS50294	WD-40 repeat
	IPR001680	1795	1820	PS50082	WD-40 repeat
ScanRegExp	IPR001680	1708	1722	PS00678	WD-40 repeat
ScanRegExp	IPR005829	1819	1844	PS00217	Sugar transporter superfamily

Primer_f
Primer_r
PCR conditions	test