FLJ00197

Product ID	ORK06736
Accession No	AK074125
Clone name	sj03274
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4297 bp) Predicted protein sequence (178 aa)
Source	Human spleen
Rouge ID	mFLJ00197 by Kazusa Mouse cDNA Project

Length: 4297 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 178 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84951	7.5e-64	100.0	FLJ00197 protei...	Homo sapiens
AAH33286	6e-61	100.0	SCML4 protein [...	Homo sapiens
BAG64585	6.4e-61	100.0	unnamed protein...	Homo sapiens
XP_001146954	4e-60	98.8	sex comb on mid...	Pan troglodytes
BAC04198	5.5e-57	98.8	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

Primer_f
Primer_r
PCR conditions	test