FLJ00205

Product ID	ORK05261
Accession No	AK074132
Clone name	sj04565
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4188 bp) Predicted protein sequence (359 aa)
Source	Human spleen
Rouge ID	mFLJ00205 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Length: 359 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84958	3.4e-156	100.0	FLJ00205 protei...	Homo sapiens
BAC56890	1.1e-150	100.0	UDP-N-acetyl-al...	Homo sapiens
Q86SR1	1.1e-150	100.0	Polypeptide N-a...	Homo sapiens
XP_001111603	2e-150	99.7	similar to GalN...	Macaca mulatta
XP_001111448	2e-150	99.7	similar to GalN...	Macaca mulatta

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB067505	7.8e-52	49.0	KIAA1918
AB032956	2.4e-46	42.1	KIAA1130

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001173	141	326	PF00535	Glycosyl transferase
ProfileScan	IPR001173	139	244	PS50167	Glycosyl transferase

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	2	LQAVALVLAALVLLPNVGLWALY	24	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test