FLJ00219

Product ID	ORK07088
Accession No	AK074146
Clone name	sj05764
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4498 bp) Predicted protein sequence (168 aa)
Source	Human spleen

Length: 4498 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 168 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84972	6.5e-69	100.0	FLJ00219 protei...	Homo sapiens
XP_001172987	8.5e-32	72.8	similar to FLJ0...	Pan troglodytes
XP_001713686	4.2e-27	97.5	hypothetical pr...	Homo sapiens
BAC03977	1e-26	96.2	unnamed protein...	Homo sapiens
AAH73934	2.2e-26	96.2	Similar to hypo...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

Primer_f
Primer_r
PCR conditions	test