FLJ00239

Product ID	ORK05130
Accession No	AK074166
Clone name	sj07427
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4512 bp) Predicted protein sequence (717 aa)
Source	Human spleen

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	Warning

Length: 717 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAB84992	100.0	FLJ00239 protei...	Homo sapiens
Q2M385	100.0	Macrophage-expr...	Homo sapiens
BAG53544	99.7	unnamed protein...	Homo sapiens
AAI12231	99.9	Macrophage expr...	Homo sapiens
XP_508450	99.3	macrophage expr...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001862	143	344	PF01823	Membrane attack complex component/perforin/complement C9
HMMSmart	IPR001862	145	344	SM00457	Membrane attack complex component/perforin/complement C9

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	1	AMNNFRATILFWAAAAWAKSGKP	23	SECONDARY	23
	2	571	DGCQVSYCVKSGLFTGGSLPPAR	593	SECONDARY	23
	3	656	GAAAGVTVGVTTILAVVITLAIY	678	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test