HUGE

Gene/Protein Characteristic Table for KIAA0476

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Mapping

Product ID :	ORK00087
Accession No. :	AB007945
Description :	DENN/MADD domain containing 4B.
HUGO Gene Name :
Clone Name :	ah04654 [Vector Info]
Flexi ORF Clone :	pF1KA0476
Source :	Human brain (amygdala)
Note :	We replaced hh00487, former representative clones for KIAA0476 with ah04654. (2005/08/06)

Features of the cloned DNA sequence	Description

Length: 5688 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 796 bp

Genome contig ID gi89161185r_152068601
PolyA signal sequence
(AGTAAA,-25)
+----*----+----*----+----*----+----
ATGTAAATATAGTAAAAGCTGCTTCTGTCTTTTTC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
CTTCGGCCTCCTGTTCCCTGGGTCTGGGGTAATGCGGGGATACCTTTCAT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 r 152168601 152185778 28 99.9 Perfect prediction

Features of the protein sequence	Description

Length: 1626 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_513830	97.3	DENN/MADD domai...	Pan troglodytes
O75064	100.0	DENN domain-con...	Homo sapiens
XP_001493908	95.2	DENN/MADD domai...	Equus caballus
XP_001929547	94.8	DENN/MADD domai...	Sus scrofa
BAE33359	93.6	unnamed protein...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB051553	1.2e-08	28.4	KIAA1766
AB029014	3.8e-05	24.2	KIAA1091
AB033103	0.00023	24.7	KIAA1277

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR005113 321 409 PF03456 uDENN

IPR001194 443 627 PF02141 DENN

IPR005112 692 766 PF03455 dDENN

IPR002885 944 978 PF01535 Pentatricopeptide repeat

HMMSmart IPR005113 302 409 SM00800 uDENN

IPR001194 443 627 SM00799 DENN

IPR005112 692 766 SM00801 dDENN

HMMTigr IPR002885 944 978 TIGR00756 Pentatricopeptide repeat

ProfileScan IPR005113 136 415 PS50946 uDENN

IPR001194 443 627 PS50211 DENN

IPR005112 692 766 PS50947 dDENN

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR005113	321	409	PF03456	uDENN
IPR001194	443	627	PF02141	DENN
IPR005112	692	766	PF03455	dDENN
IPR002885	944	978	PF01535	Pentatricopeptide repeat
HMMSmart	IPR005113	302	409	SM00800	uDENN
IPR001194	443	627	SM00799	DENN
IPR005112	692	766	SM00801	dDENN
HMMTigr	IPR002885	944	978	TIGR00756	Pentatricopeptide repeat
ProfileScan	IPR005113	136	415	PS50946	uDENN
IPR001194	443	627	PS50211	DENN
IPR005112	692	766	PS50947	dDENN

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 527 SFLQLLQSLGPELAITLLLAVLT 549 SECONDARY 23

2 560 PDLLTSVCEALVSMIFPLHWQCP 582 SECONDARY 23

3 586 LCPLVLADVLSAPVPFIVGIHSS 608 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	527	SFLQLLQSLGPELAITLLLAVLT	549	SECONDARY	23
2	560	PDLLTSVCEALVSMIFPLHWQCP	582	SECONDARY	23
3	586	LCPLVLADVLSAPVPFIVGIHSS	608	PRIMARY	23

RH mapping information	Description

Chromosome No.

: 1

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CTCCATAAGAACCTCATAAGC
Primer_r	: AATAATGGTCCCTCTTCCCTG
PCR product length	: 135 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	796 bp
Genome contig ID	gi89161185r_152068601
PolyA signal sequence (AGTAAA,-25)	+--------+--------+----*----+---- ATGTAAATATAGTAAAAGCTGCTTCTGTCTTTTTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* CTTCGGCCTCCTGTTCCCTGGGTCTGGGGTAATGCGGGGATACCTTTCAT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	r	152168601	152185778	28	99.9	Perfect prediction

Gene/Protein Characteristic Table for KIAA0476

Features of the cloned DNA sequence

Features of the protein sequence

RH mapping information