HUGE

Gene/Protein Characteristic Table for KIAA1144

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00186
Accession No. :	AB032970
Description :	Potassium voltage-gated channel subfamily S member 2.
HUGO Gene Name :
Clone Name :	hj05565 [Vector Info]
Flexi ORF Clone :	pF1KA1144
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 5027 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 3438 bp

Genome contig ID gi51511724f_99408621
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
ACACTACTGTGCAAGCATTGACACAGGCTGCACTG
Flanking genome sequence
(105633 - 105682)
----+----*----+----*----+----*----+----*----+----*
AACCATTATGTCTGATTGCTGCACTTTCATCTCTGTGCCTCGATTCGGGA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 8 f 99508621 99514252 2 99.0 Perfect prediction

Features of the protein sequence	Description

Length: 489 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9ULS6	9.6e-207	100.0	Potassium volta...	Homo sapiens
XP_001094800	2.9e-206	100.0	potassium volta...	Macaca mulatta
XP_001928892	1.9e-205	99.4	similar to Pota...	Sus scrofa
XP_592772	6.6e-205	99.0	similar to Pota...	Bos taurus
XP_539100	9.5e-205	96.5	similar to Pota...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB028967	7.2e-32	30.3	KIAA1044
AB037777	8.4e-05	26.2	KIAA1356
D79998	0.00023	32.6	KIAA0176

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

FPrintScan IPR003971 34 46 PR01494 Kv9 voltage-gated K+ channel

IPR003971 49 65 PR01494 Kv9 voltage-gated K+ channel

IPR003971 72 85 PR01494 Kv9 voltage-gated K+ channel

IPR003968 80 90 PR01491 Kv channel

IPR003091 80 99 PR00169 Voltage-dependent potassium channel

IPR003971 96 111 PR01494 Kv9 voltage-gated K+ channel

IPR003971 119 131 PR01494 Kv9 voltage-gated K+ channel

IPR003091 192 220 PR00169 Voltage-dependent potassium channel

IPR003971 205 217 PR01494 Kv9 voltage-gated K+ channel

IPR003091 241 264 PR00169 Voltage-dependent potassium channel

IPR003091 267 287 PR00169 Voltage-dependent potassium channel

IPR003091 311 337 PR00169 Voltage-dependent potassium channel

IPR003968 316 324 PR01491 Kv channel

IPR003968 340 354 PR01491 Kv channel

IPR003091 340 363 PR00169 Voltage-dependent potassium channel

IPR003091 370 392 PR00169 Voltage-dependent potassium channel

IPR003091 399 425 PR00169 Voltage-dependent potassium channel

IPR003971 409 427 PR01494 Kv9 voltage-gated K+ channel

IPR003968 410 421 PR01491 Kv channel

HMMPfam IPR003131 31 130 PF02214 K+ channel tetramerisation

IPR005821 241 421 PF00520 Ion transport

HMMSmart IPR000210 29 138 SM00225 BTB/POZ-like

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR003971	34	46	PR01494	Kv9 voltage-gated K+ channel
IPR003971	49	65	PR01494	Kv9 voltage-gated K+ channel
IPR003971	72	85	PR01494	Kv9 voltage-gated K+ channel
IPR003968	80	90	PR01491	Kv channel
IPR003091	80	99	PR00169	Voltage-dependent potassium channel
IPR003971	96	111	PR01494	Kv9 voltage-gated K+ channel
IPR003971	119	131	PR01494	Kv9 voltage-gated K+ channel
IPR003091	192	220	PR00169	Voltage-dependent potassium channel
IPR003971	205	217	PR01494	Kv9 voltage-gated K+ channel
IPR003091	241	264	PR00169	Voltage-dependent potassium channel
IPR003091	267	287	PR00169	Voltage-dependent potassium channel
IPR003091	311	337	PR00169	Voltage-dependent potassium channel
IPR003968	316	324	PR01491	Kv channel
IPR003968	340	354	PR01491	Kv channel
IPR003091	340	363	PR00169	Voltage-dependent potassium channel
IPR003091	370	392	PR00169	Voltage-dependent potassium channel
IPR003091	399	425	PR00169	Voltage-dependent potassium channel
IPR003971	409	427	PR01494	Kv9 voltage-gated K+ channel
IPR003968	410	421	PR01491	Kv channel
HMMPfam	IPR003131	31	130	PF02214	K+ channel tetramerisation
IPR005821	241	421	PF00520	Ion transport
HMMSmart	IPR000210	29	138	SM00225	BTB/POZ-like

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 197 LSRVFSILSILVVMGSIITMCLN 219 PRIMARY 23

2 243 EHFGIAWFTFELVARFAVAPDFL 265 SECONDARY 23

3 273 NLIDLMSIVPFYITLVVNLVVES 295 PRIMARY 23

4 337 SYKEVGLLLLYLSVGISIFSVVA 359 SECONDARY 23

5 367 NEGLATIPACWWWATVSMTTVGY 389 SECONDARY 23

6 403 ASACILAGILVVVLPITLIFNKF 425 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	197	LSRVFSILSILVVMGSIITMCLN	219	PRIMARY	23
2	243	EHFGIAWFTFELVARFAVAPDFL	265	SECONDARY	23
3	273	NLIDLMSIVPFYITLVVNLVVES	295	PRIMARY	23
4	337	SYKEVGLLLLYLSVGISIFSVVA	359	SECONDARY	23
5	367	NEGLATIPACWWWATVSMTTVGY	389	SECONDARY	23
6	403	ASACILAGILVVVLPITLIFNKF	425	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: GGTTGGGAAGTGACACGTCTG

Primer_r
: ACCAATTACTACCTTACGAGC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 8

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: GGTTGGGAAGTGACACGTCTG
Primer_r	: ACCAATTACTACCTTACGAGC
PCR product length	: 148 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	3438 bp
Genome contig ID	gi51511724f_99408621
PolyA signal sequence (None)	+--------+--------+----*----+---- ACACTACTGTGCAAGCATTGACACAGGCTGCACTG
Flanking genome sequence (105633 - 105682)	----+--------+--------+--------+--------+----* AACCATTATGTCTGATTGCTGCACTTTCATCTCTGTGCCTCGATTCGGGA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	8	f	99508621	99514252	2	99.0	Perfect prediction

Gene/Protein Characteristic Table for KIAA1144

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information