HUGE

Gene/Protein Characteristic Table for KIAA1170

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB032996
Description :	Protein FAM40B.
HUGO Gene Name :	family with sequence similarity 40, member B (FAM40B)
Clone Name :	hg04224 [Vector Info]
Flexi ORF Clone :	pF1KA1170
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 6645 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
YES YES

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 2554 bp

Genome contig ID gi89161213f_128761536
PolyA signal sequence
(AATAAA,-21)
+----*----+----*----+----*----+----
AGCACTTTAATAAAAATAAAATGAGCTGGAATTAG
Flanking genome sequence None

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 7 f 128861536 128979750 25 98.7 Both No-hit

Features of the protein sequence	Description

Length: 838 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAL24101	100.0	family with seq...	Homo sapiens
XP_001093257	99.5	similar to Prot...	Macaca mulatta
Q9ULQ0	100.0	Protein FAM40B.	Homo sapiens
BAF82854	99.9	unnamed protein...	Homo sapiens
XP_849003	97.7	similar to fami...	Canis lupus fam...

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB051548	1.3e-115	70.0	KIAA1761

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR012486 37 358 PF07923 N1221-like

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR012486	37	358	PF07923	N1221-like

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 273 VLLLLWKVVMFTLGGFEHLQTLK 295 PRIMARY 23

2 512 GMLYSLPQYMIALLKILLAAAP 533 PRIMARY 22

3 571 KEIIVKSISTLLLLLLKHFKLNH 593 SECONDARY 23

4 597 FEYVSQHLVFANCIPLILKFFNQ 619 SECONDARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	273	VLLLLWKVVMFTLGGFEHLQTLK	295	PRIMARY	23
2	512	GMLYSLPQYMIALLKILLAAAP	533	PRIMARY	22
3	571	KEIIVKSISTLLLLLLKHFKLNH	593	SECONDARY	23
4	597	FEYVSQHLVFANCIPLILKFFNQ	619	SECONDARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: ACTACAGGAGGCTTTATTCAG

Primer_r
: TGCTAAATCTCCACATGACAC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 7

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: ACTACAGGAGGCTTTATTCAG
Primer_r	: TGCTAAATCTCCACATGACAC
PCR product length	: 175 bp
PCR conditions	: 95 °C15 sec62 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	YES	YES
Warning for coding interruption:	YES	NO

Length of 3'UTR	2554 bp
Genome contig ID	gi89161213f_128761536
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- AGCACTTTAATAAAAATAAAATGAGCTGGAATTAG
Flanking genome sequence	None

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	7	f	128861536	128979750	25	98.7	Both No-hit

Gene/Protein Characteristic Table for KIAA1170

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information