HUGE

Gene/Protein Characteristic Table for KIAA1278

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :
Accession No. :	AB033104
Description :	Serine/threonine-protein kinase 36.
HUGO Gene Name :	serine/threonine kinase 36, fused homolog (Drosophila) (STK36)
Clone Name :	hg03689 [Vector Info]
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 5110 bp
Physical map
Restriction map
The cloned DNA sequence was revised by direct RT-PCR/sequencing experiments following the alert of coding interruption by GeneMark analysis.
Comparison between the cloned and the revised DNA sequences.
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq. revised DNA seq.
Warning for N-terminal truncation:
NO NO

Warning for coding interruption:
YES NO

Integrity of 3' end

Length of 3'UTR 700 bp

Genome contig ID gi89161199f_219145036
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
TTCTATGTGGTGTTGGAGGTGCTGGTATCGTGTTC
Flanking genome sequence
(130642 - 130691)
----+----*----+----*----+----*----+----*----+----*
ACACAAGATCCTGGTCTGGCAGCTTATTTATATCATTTCTTGCCTTTACC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 2 f 219245036 219275676 26 99.8 Perfect prediction

Features of the protein sequence	Description

Length: 1311 aa
This protein sequence is predicted from the revised DNA sequence

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW70648	99.6	serine/threonin...	Homo sapiens
XP_001093220	96.5	similar to seri...	Macaca mulatta
AAF97028	98.3	FUSED serine/th...	Homo sapiens
Q9NRP7	98.4	Serine/threonin...	Homo sapiens
EAW70647	97.4	serine/threonin...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB067488	3.5e-23	35.9	KIAA1901
D86959	3.8e-22	36.0	KIAA0204
AB058763	5.5e-22	29.5	KIAA1860
AB011109	1.3e-21	25.2	KIAA0537
AB032968	1.6e-21	35.3	KIAA1142

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

BlastProDom IPR000719 24 270 PD000001 Protein kinase

HMMPfam IPR000719 21 271 PF00069 Protein kinase

IPR000357 1105 1136 PF02985 HEAT

IPR000357 1143 1179 PF02985 HEAT

IPR000357 1183 1219 PF02985 HEAT

HMMSmart IPR001245 21 271 SM00219 Tyrosine protein kinase

IPR002290 21 271 SM00220 Serine/threonine protein kinase

ProfileScan IPR000719 21 271 PS50011 Protein kinase

ScanRegExp IPR000719 27 50 PS00107 Protein kinase

IPR008271 138 150 PS00108 Serine/threonine protein kinase

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR000719	24	270	PD000001	Protein kinase
HMMPfam	IPR000719	21	271	PF00069	Protein kinase
IPR000357	1105	1136	PF02985	HEAT
IPR000357	1143	1179	PF02985	HEAT
IPR000357	1183	1219	PF02985	HEAT
HMMSmart	IPR001245	21	271	SM00219	Tyrosine protein kinase
IPR002290	21	271	SM00220	Serine/threonine protein kinase
ProfileScan	IPR000719	21	271	PS50011	Protein kinase
ScanRegExp	IPR000719	27	50	PS00107	Protein kinase
IPR008271	138	150	PS00108	Serine/threonine protein kinase

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 723 QHPILCLHLLKVLYSCCLVSEGL 745 PRIMARY 23

2 804 VATLFTHSHVVSLVSAAACLLGQ 826 SECONDARY 23

3 969 GSEFLPVVVLSVCQLLCFPFALD 991 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	723	QHPILCLHLLKVLYSCCLVSEGL	745	PRIMARY	23
2	804	VATLFTHSHVVSLVSAAACLLGQ	826	SECONDARY	23
3	969	GSEFLPVVVLSVCQLLCFPFALD	991	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CACCATACTTGAAGCTCCCAG

Primer_r
: GATACCAGCACCTCCAACACC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 2

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CACCATACTTGAAGCTCCCAG
Primer_r	: GATACCAGCACCTCCAACACC
PCR product length	: 84 bp
PCR conditions	: 95 °C15 sec66 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.	revised DNA seq.
Warning for N-terminal truncation:	NO	NO
Warning for coding interruption:	YES	NO

Length of 3'UTR	700 bp
Genome contig ID	gi89161199f_219145036
PolyA signal sequence (None)	+--------+--------+----*----+---- TTCTATGTGGTGTTGGAGGTGCTGGTATCGTGTTC
Flanking genome sequence (130642 - 130691)	----+--------+--------+--------+--------+----* ACACAAGATCCTGGTCTGGCAGCTTATTTATATCATTTCTTGCCTTTACC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	2	f	219245036	219275676	26	99.8	Perfect prediction

Gene/Protein Characteristic Table for KIAA1278

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information