HUGE

Gene/Protein Characteristic Table for KIAA1479

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00847
Accession No. :	AB040912
Description :	Semaphorin-6D precursor.
HUGO Gene Name :	sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6D (SEMA6D)
Clone Name :	fh17949 [Vector Info]
Flexi ORF Clone :	pF1KSDA1479
Source :	Human fetal brain
Note :	We replaced fj05577, former representative clones for KIAA1479 with fh17949. (2001/5/29)

Features of the cloned DNA sequence	Description

Length: 5924 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
YES

Integrity of 3' end

Length of 3'UTR 2437 bp

Genome contig ID gi51511731f_45163695
PolyA signal sequence
(AATAAA,-20)
+----*----+----*----+----*----+----
CGCATGTAAAGTCAAAATAAAATATACAAATCATT
Flanking genome sequence
(690016 - 690065)
----+----*----+----*----+----*----+----*----+----*
ATATCCTGCCTCTTGATATTGAAGAGGTTTATAACATGTTATATTTTTAA

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 15 f 45263695 45853709 18 99.4 Internal No-hit

Features of the protein sequence	Description

Length: 1022 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
AAM69453	100.0	semaphorin 6D i...	Homo sapiens
XP_001164625	99.8	semaphorin 6D i...	Pan troglodytes
XP_001112381	99.3	similar to sema...	Macaca mulatta
XP_001502487	97.8	sema domain, tr...	Equus caballus
XP_596649	96.9	similar to sema...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB037789	6.5e-82	45.2	KIAA1368
AB058772	1.2e-71	45.3	KIAA1869
AB040878	9.6e-27	35.8	KIAA1445
AB002329	4.5e-26	34.2	KIAA0331
AB051526	1.3e-18	33.7	KIAA1739

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR001627 70 507 PF01403 Semaphorin/CD100 antigen

IPR002165 525 593 PF01437 Plexin

HMMSmart IPR001627 68 498 SM00630 Semaphorin/CD100 antigen

IPR003659 525 593 SM00423 Plexin/semaphorin/integrin

ProfileScan IPR001627 38 523 PS51004 Semaphorin/CD100 antigen

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001627	70	507	PF01403	Semaphorin/CD100 antigen
IPR002165	525	593	PF01437	Plexin
HMMSmart	IPR001627	68	498	SM00630	Semaphorin/CD100 antigen
IPR003659	525	593	SM00423	Plexin/semaphorin/integrin
ProfileScan	IPR001627	38	523	PS51004	Semaphorin/CD100 antigen

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 11 TMRVFLLCAYILLLMVSQLRAVS 33 PRIMARY 23

2 612 VLITCVFAAFVLGAFIAGVAVYC 634 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	11	TMRVFLLCAYILLLMVSQLRAVS	33	PRIMARY	23
2	612	VLITCVFAAFVLGAFIAGVAVYC	634	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: AGTTAATATGCTGCACACCAC

Primer_r
: CTGGTCTTCTGCAACATGTCC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 15

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	YES

Length of 3'UTR	2437 bp
Genome contig ID	gi51511731f_45163695
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- CGCATGTAAAGTCAAAATAAAATATACAAATCATT
Flanking genome sequence (690016 - 690065)	----+--------+--------+--------+--------+----* ATATCCTGCCTCTTGATATTGAAGAGGTTTATAACATGTTATATTTTTAA

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	15	f	45263695	45853709	18	99.4	Internal No-hit

Primer_f	: AGTTAATATGCTGCACACCAC
Primer_r	: CTGGTCTTCTGCAACATGTCC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1479

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information