HUGE

Gene/Protein Characteristic Table for KIAA1869

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00937
Accession No. :	AB058772
Description :	Semaphorin-6C precursor.
HUGO Gene Name :	sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6C (SEMA6C)
Clone Name :	hh01800 [Vector Info]
Flexi ORF Clone :	pF1KSDA1869
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 5261 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 776 bp

Genome contig ID gi89161185r_149270808
PolyA signal sequence
(AATAAA,-26)
+----*----+----*----+----*----+----
TTTTTGAGAAATAAAATGAAGCTGCAGTGTACGTT
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
ACTTGAAGTTCCACATCAGAAAATCTTGTCCTTAGGAGAATTTGTCTGGC

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 1 r 149370808 149385711 19 100.0 Perfect prediction

Features of the protein sequence	Description

Length: 935 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
CAI16378	100.0	sema domain, tr...	Homo sapiens
BAB20670	99.9	semaphorin Y [H...	Homo sapiens
Q9H3T2	99.9	Semaphorin-6C; ...	Homo sapiens
XP_001104082	97.8	semaphorin Y is...	Macaca mulatta
XP_001929695	89.9	similar to Sema...	Sus scrofa

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB040912	3.8e-51	45.3	KIAA1479
AB037789	1.6e-43	38.6	KIAA1368
AB040878	5.7e-17	36.2	KIAA1445
AB002329	8e-14	30.8	KIAA0331
AB051526	1.2e-13	35.6	KIAA1739

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR001627 69 496 PF01403 Semaphorin/CD100 antigen

IPR002165 523 544 PF01437 Plexin

HMMSmart IPR001627 66 494 SM00630 Semaphorin/CD100 antigen

ProfileScan IPR001627 35 521 PS51004 Semaphorin/CD100 antigen

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001627	69	496	PF01403	Semaphorin/CD100 antigen
IPR002165	523	544	PF01437	Plexin
HMMSmart	IPR001627	66	494	SM00630	Semaphorin/CD100 antigen
ProfileScan	IPR001627	35	521	PS51004	Semaphorin/CD100 antigen

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 7 PRAPHFMPLLLLLLLLSLPHTQ 28 SECONDARY 22

2 610 LLLASVAAAFALGASVSGLLVSC 632 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	7	PRAPHFMPLLLLLLLLSLPHTQ	28	SECONDARY	22
2	610	LLLASVAAAFALGASVSGLLVSC	632	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: TCGCTCTCTGCCTTCATCTGG

Primer_r
: CACCGTTAATTCGCTCGTCTC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 10

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: CTCGCTCTCTGCCTTCATCTG
Primer_r	: CTTCCCCAGCCTCAACTAAAC
PCR product length	: 95 bp
PCR conditions	: 15 °C66 sec60 °C30 sec170 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	776 bp
Genome contig ID	gi89161185r_149270808
PolyA signal sequence (AATAAA,-26)	+--------+--------+----*----+---- TTTTTGAGAAATAAAATGAAGCTGCAGTGTACGTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACTTGAAGTTCCACATCAGAAAATCTTGTCCTTAGGAGAATTTGTCTGGC

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	1	r	149370808	149385711	19	100.0	Perfect prediction

Primer_f	: TCGCTCTCTGCCTTCATCTGG
Primer_r	: CACCGTTAATTCGCTCGTCTC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1869

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information