HUGE

Gene/Protein Characteristic Table for KIAA0331

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK00507
Accession No. :	AB002329
Description :	Semaphorin-3E precursor.
HUGO Gene Name :	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E)
Clone Name :	hg00928 [Vector Info]
Flexi ORF Clone :	pF1KSDA0331
Source :	Human adult brain

Features of the cloned DNA sequence	Description

Length: 6474 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
NO

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 3680 bp

Genome contig ID gi89161213r_82731446
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
AGTCCGGCCTGGGCGACAGAGCGAGACTCCGTCTC
Flanking genome sequence
(99712 - 99663)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAAT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 7 r 82831158 83116260 17 99.2 Perfect prediction

Features of the protein sequence	Description

Length: 814 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
O15041	100.0	Semaphorin-3E; ...	Homo sapiens
XP_527803	99.9	semaphorin 3E [...	Pan troglodytes
AAI44339	99.9	Sema domain, im...	Homo sapiens
BAG59693	98.6	unnamed protein...	Homo sapiens
BAG64853	100.0	unnamed protein...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB051526	2.8e-55	33.8	KIAA1739
AB040912	1.1e-49	34.2	KIAA1479
AB051532	2.8e-47	30.6	KIAA1745
AB046839	1.8e-44	31.6	KIAA1619
AB037789	1.1e-40	31.8	KIAA1368

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR001627 97 539 PF01403 Semaphorin/CD100 antigen

IPR013151 634 695 PF00047 Immunoglobulin

HMMSmart IPR001627 97 539 SM00630 Semaphorin/CD100 antigen

IPR003659 557 612 SM00423 Plexin/semaphorin/integrin

ProfileScan IPR001627 71 555 PS51004 Semaphorin/CD100 antigen

IPR007110 633 708 PS50835 Immunoglobulin-like

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001627	97	539	PF01403	Semaphorin/CD100 antigen
IPR013151	634	695	PF00047	Immunoglobulin
HMMSmart	IPR001627	97	539	SM00630	Semaphorin/CD100 antigen
IPR003659	557	612	SM00423	Plexin/semaphorin/integrin
ProfileScan	IPR001627	71	555	PS51004	Semaphorin/CD100 antigen
IPR007110	633	708	PS50835	Immunoglobulin-like

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 39 SMASAGHIITLLLWGYLLELWTG 61 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	39	SMASAGHIITLLLWGYLLELWTG	61	PRIMARY	23

Expression profile	Description

: RT-PCR Description

Experimental conditions

Primer_f
: GCGCTCTAGACAGGACATGGC

Primer_r
: GACAGTTGTTTATAAGCCGGG

PCR conditions
: 95 °C30 sec61 °C30 sec72 °C60 sec30 cycles

RT-PCR	Description

RH mapping information	Description

Chromosome No.

: 7

Experimental conditions

Panel name	: GeneBridge 4
Primer_f	: TGTTGTGAGAAATGCCCAGGC
Primer_r	: TTGGTGAGATGAAGGGTGAAC
PCR product length	: 126 bp
PCR conditions	: 95 °C15 sec64 °C60 sec30 cycles

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	NO
Warning for coding interruption:	NO

Length of 3'UTR	3680 bp
Genome contig ID	gi89161213r_82731446
PolyA signal sequence (None)	+--------+--------+----*----+---- AGTCCGGCCTGGGCGACAGAGCGAGACTCCGTCTC
Flanking genome sequence (99712 - 99663)	----+--------+--------+--------+--------+----* AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATGAAT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	7	r	82831158	83116260	17	99.2	Perfect prediction

Primer_f	: GCGCTCTAGACAGGACATGGC
Primer_r	: GACAGTTGTTTATAAGCCGGG
PCR conditions	: 95 °C30 sec61 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA0331

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information