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SpecieshumanProduct IDFHC00428Cloning SiteSgfI-PmeISymbolSCRIB
Alias : CRIB1, SCRB1, SCRIB1, VartulDescriptionscribbled planar cell polarity protein, transcript variant 2
Characterization of the cloned ORF
N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand
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Length: 4890 bp
Restriction map A
Restriction map B
Genomic Structure
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Length: 1630 aa
Result of homology search against CCDS protein database (
FASTA output,
Multiple alignment)
Entry Exp ID% symbol CCDS6411.1 0 99.9 SCRIB CCDS6412.1 0 98.4 SCRIB CCDS4953.2 9.1e-51 64.5 LRRC1 CCDS34172.1 9.2e-30 39.2 ERBIN CCDS58954.1 9.4e-30 39.2 ERBIN
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
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Result of InterProScan
Search method interpro_ID From To Entry Definition HMMPfam IPR001611 175 196 PF00560 Leucine-rich repeat IPR001611 198 219 PF00560 Leucine-rich repeat IPR001478 730 811 PF00595 PDZ/DHR/GLGF IPR001478 868 946 PF00595 PDZ/DHR/GLGF IPR001478 1005 1089 PF00595 PDZ/DHR/GLGF IPR001478 1104 1188 PF00595 PDZ/DHR/GLGF HMMSmart NULL 35 54 SM00364 NULL IPR003591 58 80 SM00369 Leucine-rich repeat IPR003591 81 104 SM00369 Leucine-rich repeat NULL 83 100 SM00364 NULL IPR003591 127 149 SM00369 Leucine-rich repeat IPR003591 150 172 SM00369 Leucine-rich repeat NULL 150 169 SM00364 NULL IPR003591 173 195 SM00369 Leucine-rich repeat NULL 173 192 SM00364 NULL IPR003591 196 218 SM00369 Leucine-rich repeat NULL 196 216 SM00364 NULL IPR003591 219 241 SM00369 Leucine-rich repeat NULL 219 238 SM00364 NULL IPR003591 242 265 SM00369 Leucine-rich repeat NULL 242 261 SM00364 NULL NULL 265 284 SM00364 NULL IPR003591 311 334 SM00369 Leucine-rich repeat NULL 311 330 SM00364 NULL IPR003591 335 356 SM00369 Leucine-rich repeat IPR003591 357 380 SM00369 Leucine-rich repeat NULL 357 376 SM00364 NULL IPR001478 736 815 SM00228 PDZ/DHR/GLGF IPR001478 870 950 SM00228 PDZ/DHR/GLGF IPR001478 1012 1093 SM00228 PDZ/DHR/GLGF IPR001478 1109 1192 SM00228 PDZ/DHR/GLGF ProfileScan IPR001611 37 58 PS51450 Leucine-rich repeat IPR001611 60 81 PS51450 Leucine-rich repeat IPR001611 83 104 PS51450 Leucine-rich repeat IPR001611 106 127 PS51450 Leucine-rich repeat IPR001611 152 174 PS51450 Leucine-rich repeat NULL 175 197 PS51450 NULL IPR001611 198 219 PS51450 Leucine-rich repeat IPR001611 221 243 PS51450 Leucine-rich repeat IPR001611 244 265 PS51450 Leucine-rich repeat IPR001611 290 312 PS51450 Leucine-rich repeat IPR001611 313 334 PS51450 Leucine-rich repeat IPR001611 336 357 PS51450 Leucine-rich repeat IPR001611 359 381 PS51450 Leucine-rich repeat IPR001478 728 815 PS50106 PDZ/DHR/GLGF IPR001478 862 950 PS50106 PDZ/DHR/GLGF IPR001478 1004 1093 PS50106 PDZ/DHR/GLGF IPR001478 1100 1188 PS50106 PDZ/DHR/GLGF
Result of homology search against disease genes in OMIM database(FASTA output,
Multiple alignment)
H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_056171 1630 607733 99.9 100.0 NP_874365 1655 607733 98.4 100.0 Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.comCloned ORF Nucleotide sequence for pFN21ASDA0147 Download>KIAA0147 4890 bp ATGCTCAAGTGCATCCCGCTGTGGCGCTGCAACCGGCACGTGGAGTCGGTGGACAAGCGG CACTGTTCGCTGCAGGCCGTGCCGGAGGAGATCTACCGCTACAGCCGCAGCCTGGAGGAG CTGCTGCTCGACGCCAACCAGCTGCGCGAGCTGCCCAAGCCTTTTTTCCGGCTGCTGAAC TTGCGCAAGCTGGGCCTGAGCGACAACGAGATCCAGCGGTTGCCTCCCGAGGTGGCCAAC TTCATGCAGCTGGTGGAGCTGGACGTGTCCCGGAACGATATCCCTGAGATCCCGGAGAGC ATCAAGTTCTGCAAGGCTCTGGAGATCGCGGACTTCAGCGGGAACCCCCTCTCCAGGCTC CCTGATGGCTTCACTCAGCTGCGCAGCCTGGCTCACCTGGCCCTGAATGATGTGTCTCTG CAGGCACTGCCCGGGGACGTGGGCAACCTCGCCAACCTGGTGACCCTGGAGCTCCGGGAG AACCTGCTCAAGTCCCTGCCAGCGTCCCTGTCATTTCTGGTCAAGCTGGAACAGCTGGAT CTGGGAGGCAACGATCTGGAAGTGCTGCCAGACACTCTGGGGGCTCTGCCCAATCTTCGG GAGCTGTGGCTTGACCGGAACCAGCTGTCAGCACTGCCCCCGGAGCTCGGGAACCTGCGG CGCCTGGTGTGCCTGGACGTGTCGGAAAACCGGCTGGAGGAGCTGCCTGCTGAGCTCGGC GGGCTGGTGCTGCTCACTGACCTGCTGCTGTCCCAGAACCTGCTGCGGAGGCTGCCCGAC GGCATCGGTCAGCTGAAGCAGCTATCCATCCTAAAGGTAGACCAGAATCGGCTGTGCGAG GTGACTGAGGCCATCGGGGACTGTGAGAACCTCTCTGAGCTGATCCTCACGGAGAACCTG CTGATGGCCCTGCCCCGCTCCCTGGGAAAGCTGACTAAGCTGACCAACCTCAACGTGGAC CGGAACCACCTCGAGGCGCTGCCGCCCGAGATCGGGGGCTGTGTGGCACTCAGCGTCCTC TCCTTGAGGGACAACCGCCTGGCCGTCCTGCCACCAGAGCTGGCCCACACGACAGAGCTG CACGTGCTGGACGTGGCGGGGAACCGCCTGCAGAGTCTGCCGTTCGCGCTCACCCACCTC AATCTCAAGGCCCTGTGGCTGGCAGAGAACCAGGCGCAGCCCATGCTCCGGTTCCAGACG GAGGATGATGCCCGGACCGGCGAGAAGGTGCTCACCTGCTACTTGCTGCCCCAGCAGCCC CCACTCAGCCTCGAGGATGCTGGGCAGCAGGGGAGCCTCTCGGAGACCTGGAGCGATGCC CCGCCGAGCCGCGTCAGCGTCATCCAGTTCCTGGAGGCCCCCATAGGTGATGAGGACGCT GAGGAAGCTGCAGCTGAGAAGCGGGGCCTACAGCGCCGGGCCACACCTCACCCCAGCGAG CTCAAGGTGATGAAGAGGAGCATCGAGGGGCGGCGGAGCGAGGCCTGCCCTTGCCAGCCA GACTCTGGGTCGCCCTTGCCTGCAGAGGAGGAGAAGCGGCTGAGTGCCGAGTCTGGCCTG AGTGAAGACTCTCGCCCATCTGCCAGCACAGTCTCTGAGGCTGAGCCCGAGGGCCCGTCG GCTGAGGCACAGGGTGGGAGCCAGCAGGAAGCCACGACTGCTGGCGGGGAGGAAGACGCC GAAGAGGACTACCAGGAGCCCACGGTGCATTTCGCAGAGGACGCACTGCTGCCCGGGGAT GACAGGGAGATCGAGGAGGGGCAGCCTGAGGCCCCCTGGACCCTGCCAGGCGGGAGGCAG CGGCTCATCCGCAAGGACACACCTCACTACAAAAAGCACTTCAAGATCTCCAAGCTGCCC CAGCCCGAGGCCGTTGTGGCTCTGCTGCAGGGCATGCAGCCTGATGGGGAGGGCCCTGTG GCTCCCGGGGGCTGGCACAATGGCCCCCACGCACCCTGGGCTCCTCGGGCCCAGAAGGAG GAGGAGGAGGAGGAAGAGGGTAGTCCTCAGGAGGAGGAGGAAGAGGAGGAGGAGGAAAAC AGGGCTGAAGAGGAAGAGGCCAGCACTGAGGAGGAGGACAAGGAGGGGGCCGTGGTTTCT GCGCCCTCTGTCAAGGGAGTTTCGTTTGACCAGGCCAATAACCTGCTGATAGAGCCTGCT CGCATTGAGGAGGAAGAGCTGACCCTCACCATCCTGCGGCAGACTGGGGGCCTGGGCATC AGCATTGCGGGCGGCAAGGGCTCCACACCCTATAAGGGGGACGACGAGGGCATATTCATC TCTCGGGTGTCCGAGGAAGGCCCTGCGGCCCGGGCTGGAGTCCGTGTGGGTGACAAGCTC CTGGAGGTGAATGGTGTGGCTCTGCAGGGCGCCGAGCACCACGAGGCCGTGGAGGCGCTC CGGGGGGCCGGCACTGCCGTGCAGATGCGAGTGTGGCGGGAGCGCATGGTGGAGCCTGAG AACGCGGTCACCATCACGCCGCTGCGGCCCGAGGATGATTACAGCCCCCGAGAGCGGCGG GGAGGGGGGCTGCGCCTGCCCCTGCTCCCGCCTGAAAGCCCCGGGCCCCTCCGTCAGCGC CACGTGGCCTGCCTGGCACGCAGCGAGAGGGGGCTGGGCTTCAGCATTGCTGGTGGGAAA GGCTCCACACCCTACAGGGCTGGTGATGCGGGCATCTTCGTCTCCCGCATTGCCGAGGGC GGTGCTGCTCACCGCGCGGGCACACTGCAGGTTGGCGACCGCGTCCTCTCTATTAATGGA GTGGACGTGACTGAGGCCAGGCATGACCACGCCGTCTCCCTGCTGACCGCTGCCTCCCCC ACCATCGCCCTGCTGTTGGAGCGGGAGGCTGGGGGCCCTCTTCCTCCCAGCCCTCTGCCA CATTCCTCACCCCCCACCGCTGCTGTTGCCACCACCAGCATAACCACTGCCACCCCCGGG GTGCCTGGGTTGCCGAGCCTGGCCCCCAGCCTGCTGGCTGCCGCGTTGGAAGGGCCATAC CCAGTGGAGGAGATCCGTCTGCCAAGAGCTGGGGGCCCTCTGGGGCTTAGTATTGTCGGA GGCTCCGACCATTCCAGCCACCCGTTTGGTGTCCAGGAGCCTGGTGTGTTCATCTCCAAG GTGCTCCCGCGGGGCCTGGCCGCTCGCAGCGGCCTGCGGGTTGGGGACCGCATCCTGGCA GTGAACGGGCAAGACGTGCGGGATGCCACGCACCAAGAAGCAGTCAGTGCCCTGCTCCGG CCCTGCCTGGAGCTGTCGCTGCTGGTGCGGAGGGACCCGGCACCCCCGGGCCTACGGGAA CTGTGCATCCAGAAGGCACCTGGGGAGAGGCTGGGCATCAGCATCCGCGGGGGTGCCAGG GGCCACGCTGGCAACCCCCGCGACCCCACAGACGAGGGCATCTTCATCTCCAAGGTGAGC CCCACGGGGGCAGCCGGGCGCGACGGTCGGCTGCGTGTGGGTTTGCGGCTGTTGGAGGTG AACCAGCAGAGCCTGCTGGGCCTGACGCACGGCGAGGCGGTGCAGCTGCTCCGCAGTGTG GGCGACACCCTCACCGTGCTGGTCTGTGACGGCTTCGAGGCCAGCACCGACGCAGCCCTG GAGGTGTCCCCAGGTGTCATTGCCAACCCCTTTGCGGCAGGCATCGGCCACCGGAACAGC CTGGAGAGCATCTCTTCCATCGACCGGGAGCTGAGCCCTGAGGGCCCAGGCAAGGAGAAG GAGCTGCCTGGACAGACCCTGCACTGGGGGCCCGAGGCCACAGAAGCCGCAGGTCGGGGT CTGCAGCCCCTGAAGCTGGACTACCGCGCCCTGGCCGCCGTGCCCAGCGCTGGCAGCGTG CAGAGGGTACCGTCTGGAGCAGCTGGAGGGAAGATGGCTGAATCTCCCTGCTCCCCTAGT GGCCAGCAGCCGCCCTCCCCGCCTTCTCCGGATGAGCTGCCCGCCAATGTGAAGCAGGCC TACAGGGCCTTCGCGGCCGTGCCCACTTCTCACCCGCCTGAGGATGCCCCTGCCCAGCCC CCCACGCCTGGGCCTGCAGCCTCCCCGGAGCAGCTGTCCTTCCGGGAGCGGCAGAAGTAC TTTGAGCTGGAGGTGCGCGTGCCCCAGGCCGAGGGCCCCCCTAAGCGCGTGTCCCTGGTG GGTGCTGACGACCTGCGGAAGATGCAGGAGGAGGAAGCCAGAAAACTACAGCAGAAGAGA GCGCAGATGCTGCGGGAGGCGGCAGAGGCTGGGGCCGAAGCGAGGCTCGCCCTGGACGGG GAGACGCTGGGCGAGGAGGAACAGGAGGATGAGCAGCCACCCTGGGCCAGCCCGAGCCCC ACCTCAAGGCAGAGCCCGGCGTCCCCCCCGCCCCTGGGAGGTGGCGCCCCGGTGCGGACG GCCAAAGCTGAACGGCGCCACCAGGAGCGGCTGCGCGTGCAGAGTCCGGAGCCACCGGCA CCCGAGCGTGCCCTGTCCCCTGCCGAGCTCCGGGCCCTGGAGGCCGAGAAGCGTGCGCTG TGGAGGGCAGCCAGGATGAAGTCATTGGAACAGGACGCTCTCCGAGCACAGATGGTCCTC AGCAGGTCCCAGGAAGGCCGGGGCACGCGGGGGCCCCTGGAGCGACTGGCCGAGGCCCCT TCCCCTGCGCCCACCCCGTCGCCCACCCCTGTGGAAGACCTTGGCCCCCAGACCAGCACC TCCCCGGGACGCCTGTCACCGGACTTTGCTGAGGAGTTGAGGTCCCTGGAACCATCTCCC AGCCCTGGCCCGCAGGAGGAGGATGGAGAAGTGGCTCTGGTGCTTCTGGGCAGGCCCTCA CCCGGCGCTGTGGGCCCTGAAGATGTGGCACTGTGCAGCAGCCGCCGCCCCGTAAGGCCT GGGCGCCGTGGCCTGGGCCCTGTGCCCTCC
Cloned ORF protein sequence for pFN21ASDA0147 Download>KIAA0147 1630 aa MLKCIPLWRCNRHVESVDKRHCSLQAVPEEIYRYSRSLEELLLDANQLRELPKPFFRLLN LRKLGLSDNEIQRLPPEVANFMQLVELDVSRNDIPEIPESIKFCKALEIADFSGNPLSRL PDGFTQLRSLAHLALNDVSLQALPGDVGNLANLVTLELRENLLKSLPASLSFLVKLEQLD LGGNDLEVLPDTLGALPNLRELWLDRNQLSALPPELGNLRRLVCLDVSENRLEELPAELG GLVLLTDLLLSQNLLRRLPDGIGQLKQLSILKVDQNRLCEVTEAIGDCENLSELILTENL LMALPRSLGKLTKLTNLNVDRNHLEALPPEIGGCVALSVLSLRDNRLAVLPPELAHTTEL HVLDVAGNRLQSLPFALTHLNLKALWLAENQAQPMLRFQTEDDARTGEKVLTCYLLPQQP PLSLEDAGQQGSLSETWSDAPPSRVSVIQFLEAPIGDEDAEEAAAEKRGLQRRATPHPSE LKVMKRSIEGRRSEACPCQPDSGSPLPAEEEKRLSAESGLSEDSRPSASTVSEAEPEGPS AEAQGGSQQEATTAGGEEDAEEDYQEPTVHFAEDALLPGDDREIEEGQPEAPWTLPGGRQ RLIRKDTPHYKKHFKISKLPQPEAVVALLQGMQPDGEGPVAPGGWHNGPHAPWAPRAQKE EEEEEEGSPQEEEEEEEEENRAEEEEASTEEEDKEGAVVSAPSVKGVSFDQANNLLIEPA RIEEEELTLTILRQTGGLGISIAGGKGSTPYKGDDEGIFISRVSEEGPAARAGVRVGDKL LEVNGVALQGAEHHEAVEALRGAGTAVQMRVWRERMVEPENAVTITPLRPEDDYSPRERR GGGLRLPLLPPESPGPLRQRHVACLARSERGLGFSIAGGKGSTPYRAGDAGIFVSRIAEG GAAHRAGTLQVGDRVLSINGVDVTEARHDHAVSLLTAASPTIALLLEREAGGPLPPSPLP HSSPPTAAVATTSITTATPGVPGLPSLAPSLLAAALEGPYPVEEIRLPRAGGPLGLSIVG GSDHSSHPFGVQEPGVFISKVLPRGLAARSGLRVGDRILAVNGQDVRDATHQEAVSALLR PCLELSLLVRRDPAPPGLRELCIQKAPGERLGISIRGGARGHAGNPRDPTDEGIFISKVS PTGAAGRDGRLRVGLRLLEVNQQSLLGLTHGEAVQLLRSVGDTLTVLVCDGFEASTDAAL EVSPGVIANPFAAGIGHRNSLESISSIDRELSPEGPGKEKELPGQTLHWGPEATEAAGRG LQPLKLDYRALAAVPSAGSVQRVPSGAAGGKMAESPCSPSGQQPPSPPSPDELPANVKQA YRAFAAVPTSHPPEDAPAQPPTPGPAASPEQLSFRERQKYFELEVRVPQAEGPPKRVSLV GADDLRKMQEEEARKLQQKRAQMLREAAEAGAEARLALDGETLGEEEQEDEQPPWASPSP TSRQSPASPPPLGGGAPVRTAKAERRHQERLRVQSPEPPAPERALSPAELRALEAEKRAL WRAARMKSLEQDALRAQMVLSRSQEGRGTRGPLERLAEAPSPAPTPSPTPVEDLGPQTST SPGRLSPDFAEELRSLEPSPSPGPQEEDGEVALVLLGRPSPGAVGPEDVALCSSRRPVRP GRRGLGPVPS
Nucleotide Sequence (with vector) for pFN21ASDA0147 Download>pFN21ASDA0147 9619 bp TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGCTCAAGTGCATCCC GCTGTGGCGCTGCAACCGGCACGTGGAGTCGGTGGACAAGCGGCACTGTTCGCTGCAGGC CGTGCCGGAGGAGATCTACCGCTACAGCCGCAGCCTGGAGGAGCTGCTGCTCGACGCCAA CCAGCTGCGCGAGCTGCCCAAGCCTTTTTTCCGGCTGCTGAACTTGCGCAAGCTGGGCCT GAGCGACAACGAGATCCAGCGGTTGCCTCCCGAGGTGGCCAACTTCATGCAGCTGGTGGA GCTGGACGTGTCCCGGAACGATATCCCTGAGATCCCGGAGAGCATCAAGTTCTGCAAGGC TCTGGAGATCGCGGACTTCAGCGGGAACCCCCTCTCCAGGCTCCCTGATGGCTTCACTCA GCTGCGCAGCCTGGCTCACCTGGCCCTGAATGATGTGTCTCTGCAGGCACTGCCCGGGGA CGTGGGCAACCTCGCCAACCTGGTGACCCTGGAGCTCCGGGAGAACCTGCTCAAGTCCCT GCCAGCGTCCCTGTCATTTCTGGTCAAGCTGGAACAGCTGGATCTGGGAGGCAACGATCT GGAAGTGCTGCCAGACACTCTGGGGGCTCTGCCCAATCTTCGGGAGCTGTGGCTTGACCG GAACCAGCTGTCAGCACTGCCCCCGGAGCTCGGGAACCTGCGGCGCCTGGTGTGCCTGGA CGTGTCGGAAAACCGGCTGGAGGAGCTGCCTGCTGAGCTCGGCGGGCTGGTGCTGCTCAC TGACCTGCTGCTGTCCCAGAACCTGCTGCGGAGGCTGCCCGACGGCATCGGTCAGCTGAA GCAGCTATCCATCCTAAAGGTAGACCAGAATCGGCTGTGCGAGGTGACTGAGGCCATCGG GGACTGTGAGAACCTCTCTGAGCTGATCCTCACGGAGAACCTGCTGATGGCCCTGCCCCG CTCCCTGGGAAAGCTGACTAAGCTGACCAACCTCAACGTGGACCGGAACCACCTCGAGGC GCTGCCGCCCGAGATCGGGGGCTGTGTGGCACTCAGCGTCCTCTCCTTGAGGGACAACCG CCTGGCCGTCCTGCCACCAGAGCTGGCCCACACGACAGAGCTGCACGTGCTGGACGTGGC GGGGAACCGCCTGCAGAGTCTGCCGTTCGCGCTCACCCACCTCAATCTCAAGGCCCTGTG GCTGGCAGAGAACCAGGCGCAGCCCATGCTCCGGTTCCAGACGGAGGATGATGCCCGGAC CGGCGAGAAGGTGCTCACCTGCTACTTGCTGCCCCAGCAGCCCCCACTCAGCCTCGAGGA TGCTGGGCAGCAGGGGAGCCTCTCGGAGACCTGGAGCGATGCCCCGCCGAGCCGCGTCAG CGTCATCCAGTTCCTGGAGGCCCCCATAGGTGATGAGGACGCTGAGGAAGCTGCAGCTGA GAAGCGGGGCCTACAGCGCCGGGCCACACCTCACCCCAGCGAGCTCAAGGTGATGAAGAG GAGCATCGAGGGGCGGCGGAGCGAGGCCTGCCCTTGCCAGCCAGACTCTGGGTCGCCCTT GCCTGCAGAGGAGGAGAAGCGGCTGAGTGCCGAGTCTGGCCTGAGTGAAGACTCTCGCCC ATCTGCCAGCACAGTCTCTGAGGCTGAGCCCGAGGGCCCGTCGGCTGAGGCACAGGGTGG GAGCCAGCAGGAAGCCACGACTGCTGGCGGGGAGGAAGACGCCGAAGAGGACTACCAGGA GCCCACGGTGCATTTCGCAGAGGACGCACTGCTGCCCGGGGATGACAGGGAGATCGAGGA GGGGCAGCCTGAGGCCCCCTGGACCCTGCCAGGCGGGAGGCAGCGGCTCATCCGCAAGGA CACACCTCACTACAAAAAGCACTTCAAGATCTCCAAGCTGCCCCAGCCCGAGGCCGTTGT GGCTCTGCTGCAGGGCATGCAGCCTGATGGGGAGGGCCCTGTGGCTCCCGGGGGCTGGCA CAATGGCCCCCACGCACCCTGGGCTCCTCGGGCCCAGAAGGAGGAGGAGGAGGAGGAAGA GGGTAGTCCTCAGGAGGAGGAGGAAGAGGAGGAGGAGGAAAACAGGGCTGAAGAGGAAGA GGCCAGCACTGAGGAGGAGGACAAGGAGGGGGCCGTGGTTTCTGCGCCCTCTGTCAAGGG AGTTTCGTTTGACCAGGCCAATAACCTGCTGATAGAGCCTGCTCGCATTGAGGAGGAAGA GCTGACCCTCACCATCCTGCGGCAGACTGGGGGCCTGGGCATCAGCATTGCGGGCGGCAA GGGCTCCACACCCTATAAGGGGGACGACGAGGGCATATTCATCTCTCGGGTGTCCGAGGA AGGCCCTGCGGCCCGGGCTGGAGTCCGTGTGGGTGACAAGCTCCTGGAGGTGAATGGTGT GGCTCTGCAGGGCGCCGAGCACCACGAGGCCGTGGAGGCGCTCCGGGGGGCCGGCACTGC CGTGCAGATGCGAGTGTGGCGGGAGCGCATGGTGGAGCCTGAGAACGCGGTCACCATCAC GCCGCTGCGGCCCGAGGATGATTACAGCCCCCGAGAGCGGCGGGGAGGGGGGCTGCGCCT GCCCCTGCTCCCGCCTGAAAGCCCCGGGCCCCTCCGTCAGCGCCACGTGGCCTGCCTGGC ACGCAGCGAGAGGGGGCTGGGCTTCAGCATTGCTGGTGGGAAAGGCTCCACACCCTACAG GGCTGGTGATGCGGGCATCTTCGTCTCCCGCATTGCCGAGGGCGGTGCTGCTCACCGCGC GGGCACACTGCAGGTTGGCGACCGCGTCCTCTCTATTAATGGAGTGGACGTGACTGAGGC CAGGCATGACCACGCCGTCTCCCTGCTGACCGCTGCCTCCCCCACCATCGCCCTGCTGTT GGAGCGGGAGGCTGGGGGCCCTCTTCCTCCCAGCCCTCTGCCACATTCCTCACCCCCCAC CGCTGCTGTTGCCACCACCAGCATAACCACTGCCACCCCCGGGGTGCCTGGGTTGCCGAG CCTGGCCCCCAGCCTGCTGGCTGCCGCGTTGGAAGGGCCATACCCAGTGGAGGAGATCCG TCTGCCAAGAGCTGGGGGCCCTCTGGGGCTTAGTATTGTCGGAGGCTCCGACCATTCCAG CCACCCGTTTGGTGTCCAGGAGCCTGGTGTGTTCATCTCCAAGGTGCTCCCGCGGGGCCT GGCCGCTCGCAGCGGCCTGCGGGTTGGGGACCGCATCCTGGCAGTGAACGGGCAAGACGT GCGGGATGCCACGCACCAAGAAGCAGTCAGTGCCCTGCTCCGGCCCTGCCTGGAGCTGTC GCTGCTGGTGCGGAGGGACCCGGCACCCCCGGGCCTACGGGAACTGTGCATCCAGAAGGC ACCTGGGGAGAGGCTGGGCATCAGCATCCGCGGGGGTGCCAGGGGCCACGCTGGCAACCC CCGCGACCCCACAGACGAGGGCATCTTCATCTCCAAGGTGAGCCCCACGGGGGCAGCCGG GCGCGACGGTCGGCTGCGTGTGGGTTTGCGGCTGTTGGAGGTGAACCAGCAGAGCCTGCT GGGCCTGACGCACGGCGAGGCGGTGCAGCTGCTCCGCAGTGTGGGCGACACCCTCACCGT GCTGGTCTGTGACGGCTTCGAGGCCAGCACCGACGCAGCCCTGGAGGTGTCCCCAGGTGT CATTGCCAACCCCTTTGCGGCAGGCATCGGCCACCGGAACAGCCTGGAGAGCATCTCTTC CATCGACCGGGAGCTGAGCCCTGAGGGCCCAGGCAAGGAGAAGGAGCTGCCTGGACAGAC CCTGCACTGGGGGCCCGAGGCCACAGAAGCCGCAGGTCGGGGTCTGCAGCCCCTGAAGCT GGACTACCGCGCCCTGGCCGCCGTGCCCAGCGCTGGCAGCGTGCAGAGGGTACCGTCTGG AGCAGCTGGAGGGAAGATGGCTGAATCTCCCTGCTCCCCTAGTGGCCAGCAGCCGCCCTC CCCGCCTTCTCCGGATGAGCTGCCCGCCAATGTGAAGCAGGCCTACAGGGCCTTCGCGGC CGTGCCCACTTCTCACCCGCCTGAGGATGCCCCTGCCCAGCCCCCCACGCCTGGGCCTGC AGCCTCCCCGGAGCAGCTGTCCTTCCGGGAGCGGCAGAAGTACTTTGAGCTGGAGGTGCG CGTGCCCCAGGCCGAGGGCCCCCCTAAGCGCGTGTCCCTGGTGGGTGCTGACGACCTGCG GAAGATGCAGGAGGAGGAAGCCAGAAAACTACAGCAGAAGAGAGCGCAGATGCTGCGGGA GGCGGCAGAGGCTGGGGCCGAAGCGAGGCTCGCCCTGGACGGGGAGACGCTGGGCGAGGA GGAACAGGAGGATGAGCAGCCACCCTGGGCCAGCCCGAGCCCCACCTCAAGGCAGAGCCC GGCGTCCCCCCCGCCCCTGGGAGGTGGCGCCCCGGTGCGGACGGCCAAAGCTGAACGGCG CCACCAGGAGCGGCTGCGCGTGCAGAGTCCGGAGCCACCGGCACCCGAGCGTGCCCTGTC CCCTGCCGAGCTCCGGGCCCTGGAGGCCGAGAAGCGTGCGCTGTGGAGGGCAGCCAGGAT GAAGTCATTGGAACAGGACGCTCTCCGAGCACAGATGGTCCTCAGCAGGTCCCAGGAAGG CCGGGGCACGCGGGGGCCCCTGGAGCGACTGGCCGAGGCCCCTTCCCCTGCGCCCACCCC GTCGCCCACCCCTGTGGAAGACCTTGGCCCCCAGACCAGCACCTCCCCGGGACGCCTGTC ACCGGACTTTGCTGAGGAGTTGAGGTCCCTGGAACCATCTCCCAGCCCTGGCCCGCAGGA GGAGGATGGAGAAGTGGCTCTGGTGCTTCTGGGCAGGCCCTCACCCGGCGCTGTGGGCCC TGAAGATGTGGCACTGTGCAGCAGCCGCCGCCCCGTAAGGCCTGGGCGCCGTGGCCTGGG CCCTGTGCCCTCCTACGTAGTTTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGA GTCGACCTGCAGGCATGCAAGCTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGT TGGCTGCTGCCACCGCTGAGCAATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCA GACATGATAAGATACATTGATGAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAA TGCTTTATTTGTGAAATTTGTGATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAAT AAACAAGTTAACAACAACAATTGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGG GAGGTTTTTTTAAGCAAGTAAAACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATC CTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGT CAGCACCGTTTCTGCGGACTGGCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACT TTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATG TATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGT ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCT GTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCA CGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCC GAAGAACGTTTTCCAATGATGAGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCC CGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTG GTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTA TGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACTATC GGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTT GATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATG CCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCT TCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGC TCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCT CGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTAC ACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCC TCACTGATTAAGCATTGGTAATTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAG CTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACA TGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTT TCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGC GAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCT CTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCG TGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCA AGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACT ATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTA ACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTA ACTACGGCTACACTAGAAGGACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCT TCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTT TTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGA TCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCA TGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGC ACGCTGGATGGCCCTTCGCTGGGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATT AAGTGGGGGTAATGTGGCCTGTACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTT ATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGC CGTGATCGCGCTGAACGCGTTTTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCAC TTACTGTCTGCCCTCGTAGCCATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCG GCGTAGAGGATCGAGATCT
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