GBF1 ORF clone FHC00476

KOP
KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC00476

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Clone info.

Species

human

Clone Name

pFN21ASDA0248 Sequence (with vector) Restriction Map

Product ID

FHC00476

Flexi Clone PID

FXC00476

ORF Sequence

DNA sequence (5577 bp)
Protein sequence (1859 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB383818]

Gene

KIAA0248

Symbol

GBF1

Alias : ARF1GEF

Description

golgi brefeldin A resistant guanine nucleotide exchange factor 1, transcript variant 1

Original Clone ID

ha02775s1

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 5577 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 1859 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS7533.1 0 99.9 GBF1 CCDS5346.1 6.3e-11 43.8 CYTH3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR000904 697 884 PF01369 SEC7-like HMMSmart IPR000904 696 884 SM00222 SEC7-like ProfileScan IPR000904 692 882 PS50190 SEC7-like
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_004184 1859 603698 99.9 100.0 XP_005270318 1860 603698 99.9 100.0 NP_001186308 1855 603698 99.7 100.0 NP_001186307 1856 603698 99.7 100.0 XP_006718111 1892 603698 98.2 99.9 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA0248 Download

>KIAA0248  5577 bp
ATGGTGGATAAGAATATTTACATCATTCAAGGGGAGATTAACATTGTGGTTGGGGCCATC
AAACGAAATTCCCGATGGAGCACCCATACACCACTGGATGAAGAACGGGATCCTCTGCTG
CATAGTTTCGGTCATCTAAAGGAGGTTTTAAACAGTATAACAGAACTCTCAGAAATTGAG
CCCAATGTATTCCTTCGACCTTTTCTGGAAGTGATTCGCTCTGAAGATACCACTGGCCCT
ATCACTGGACTGGCACTCACCTCTGTCAACAAGTTCCTGTCCTATGCACTCATAGATCCC
ACCCATGAGGGCACAGCAGAGGGCATGGAGAACATGGCAGATGCTGTCACCCATGCTCGT
TTTGTGGGCACGGATCCTGCCAGTGATGAAGTTGTCCTGATGAAAATCCTTCAGGTTCTA
CGGACTCTGCTGCTAACCCCAGTGGGTGCCCACCTAACCAATGAATCTGTGTGTGAGATT
ATGCAGTCTTGCTTCCGGATCTGCTTTGAAATGAGGCTCAGTGAGTTATTGAGAAAATCC
GCAGAGCACACTCTCGTAGACATGGTGCAGCTGCTCTTCACAAGGTTACCTCAGTTTAAA
GAAGAACCCAAGAACTATGTGGGGACCAACATGAAGAAGCTGAAAATGAGAGCCGGAGGC
ATGAGTGATTCATCCAAATGGAAGAAACAGAAGAGATCCCCTCGGCCCCCACGCCATATG
ACCAAAGTCACACCAGGTTCAGAGCTGCCCACTCCCAATGGAACCACCTTATCATCTAAC
CTCACTGGTGGCATGCCCTTCATTGATGTGCCCACTCCCATCTCCTCTGCAAGTTCAGAA
GCTGCCTCAGCAGTGGTCAGTCCCTCTACAGACAGTGGCCTGGAATTCTCCTCCCAAACC
ACTTCCAAGGAAGACCTTACTGATCTAGAGCAACCTGGCTCTCCAGGGTACAGCACAGCT
ACAGAGCCTGGAAGCAGTGAGCTAGGTGTTCCCGAGCAGCCTGACCTCCAGGAAGGGACC
CATGTGGAAAAGTCCCAGTCAGCATCTGTGGAGTCCATCCCTGAAGTGTTAGAGGAGTGC
ACGTCCCCTGCCGACCACTCTGACTCTGCCTCTGTCCATGACATGGATTACGTCAATCCC
CGGGGCGTGCGCTTTACACAGTCCTCCCAGAAAGAAGGCACAGCTTTGGTCCCCTATGGT
CTTCCCTGCATCCGCGAGCTCTTCCGCTTCCTCATCTCCCTCACCAATCCACACGACCGC
CATAACTCAGAGGTTATGATTCACATGGGACTGCATTTGCTGACAGTGGCCCTTGAGTCA
GCCCCTGTAGCCCAGTGCCAGACCCTCTTGGGCCTCATCAAGGATGAGATGTGCCGTCAC
TTATTCCAGCTACTCAGCATAGAGCGACTAAACCTTTATGCTGCTTCCCTGCGAGTATGC
TTCCTACTGTTTGAGAGCATGCGAGAGCACCTCAAGTTCCAAATGGAGATGTACATCAAA
AAGCTTATGGAGATCATCACTGTGGAGAACCCCAAGATGCCTTATGAGATGAAGGAGATG
GCACTGGAGGCCATTGTGCAGCTCTGGCGCATCCCCAGCTTTGTCACAGAGCTCTACATC
AACTATGATTGTGACTACTACTGTTCCAACCTCTTTGAGGAACTCACAAAGCTGCTGTCC
AAGAATGCCTTCCCTGTGTCTGGTCAACTCTATACAACACACCTACTATCTCTTGATGCC
CTATTGACAGTGATTGACAGCACCGAGGCCCACTGCCAGGCTAAAGTCCTCAACAGCCTC
ACCCAGCAAGAGAAGAAGGAGACAGCCAGACCAAGCTGTGAGATAGTAGATGGCACCCGA
GAAGCTAGCAATACTGAGAGAACTGCCAGCGATGGGAAAGCTGTAGGCATGGCCTCAGAC
ATCCCAGGCCTGCATCTGCCAGGTGGAGGGCGGCTGCCACCAGAACATGGGAAATCAGGA
TGCAGTGATCTGGAGGAAGCTGTTGACTCTGGGGCTGACAAAAAGTTTGCCCGGAAGCCA
CCCCGATTTTCCTGTCTCCTGCCAGATCCACGGGAACTAATTGAAATTAAAAACAAAAAG
AAGCTGCTAATCACTGGCACAGAGCAGTTCAATCAGAAACCAAAGAAGGGGATTCAGTTT
CTGCAAGAGAAAGGCCTCCTCACCATCCCAATGGACAACACAGAGGTTGCTCAGTGGCTC
CGAGAGAACCCTCGGCTGGACAAGAAGATGATTGGAGAGTTTGTGAGTGACCGCAAAAAC
ATTGACCTGTTGGAGAGCTTTGTGAGCACCTTCAGTTTTCAGGGTCTGCGACTGGACGAA
GCCCTCCGCCTCTACCTGGAAGCCTTCCGTTTGCCTGGGGAAGCACCAGTCATCCAGAGG
TTGCTGGAGGCATTCACAGAGCGTTGGATGAATTGTAATGGCTCCCCATTTGCCAATAGC
GATGCCTGCTTTTCCCTGGCCTATGCTGTCATCATGCTTAATACTGACCAGCACAACCAC
AATGTTCGTAAACAGAATGCACCCATGACCCTGGAGGAGTTTCGCAAAAATCTGAAAGGT
GTGAATGGAGGCAAGGACTTTGAGCAAGACATCCTGGAGGACATGTACCATGCCATCAAG
AATGAGGAAATTGTAATGCCTGAGGAGCAGACAGGCTTGGTTCGGGAGAACTATGTGTGG
AATGTGCTGCTTCATCGAGGTGCCACCCCTGAGGGCATATTCCTGCGTGTGCCTACTGCC
AGCTATGATCTTGACCTCTTCACCATGACCTGGGGCCCCACTATTGCTGCTCTCTCTTAT
GTCTTTGACAAAAGCCTTGAGGAGACAATCATCCAGAAAGCCATCTCAGGCTTCAGGAAG
TGCGCCATGATCTCCGCCCACTATGGCCTCAGCGATGTGTTTGACAATCTCATCATCTCT
CTATGCAAATTCACAGCTCTCAGCAGTGAGTCTATTGAGAACCTGCCCAGTGTATTTGGA
AGCAACCCTAAAGCCCATATTGCAGCCAAGACAGTATTCCATTTGGCCCATCGTCATGGT
GACATCCTGCGGGAGGGCTGGAAGAATATCATGGAGGCCATGCTGCAGCTCTTCCGAGCC
CAACTACTGCCCAAGGCTATGATAGAGGTAGAAGATTTCGTGGATCCCAATGGCAAGATC
TCTCTACAGCGGGAAGAGACACCATCAAACCGAGGAGAGTCAACAGTGCTGAGCTTTGTG
AGCTGGCTAACACTGAGTGGTCCTGAGCAGTCTAGTGTTCGGGGCCCATCCACTGAAAAC
CAAGAGGCCAAGAGAGTGGCCTTAGAGTGTATAAAGCAATGTGACCCAGAAAAAATGATC
ACAGAAAGCAAGTTCCTCCAGCTGGAGTCACTACAGGAGCTCATGAAGGCTCTGGTCTCA
GTGACACCAGATGAAGAGACATATGATGAGGAAGATGCTGCTTTCTGCCTAGAGATGCTG
CTAAGGATTGTGTTGGAGAACAGGGATCGTGTGGGCTGTGTGTGGCAGACTGTTCGAGAC
CATCTATACCACCTCTGTGTTCAGGCACAAGATTTCTGCTTCCTTGTGGAGCGGGCAGTG
GTGGGGTTGCTACGCCTGGCCATTCGGCTTCTCCGGAGAGAAGAGATCAGTGCTCAGGTG
CTGCTCTCCCTGCGCATTTTGCTACTGATGAAGCCCAGTGTGCTATCCCGAGTCAGCCAC
CAGGTTGCGTATGGGCTCCATGAACTCCTGAAGACCAATGCAGCCAACATCCACTCAGGT
GATGACTGGGCCACACTCTTCACACTGCTGGAGTGCATCGGCTCAGGTGTGAAGCCTCCA
GCTGCTCTGCAGGCCACAGCCAGGGCAGATGCACCTGATGCCGGGGCCCAGTCAGATAGT
GAGCTCCCATCCTACCATCAGAATGACGTGAGCCTGGATCGAGGGTACACTTCCGACTCA
GAGGTCTACACTGACCATGGCAGGCCGGGCAAGATACACCGATCAGCCACAGATGCCGAT
GTGGTCAACAGTGGTTGGTTAGTGGTTGGGAAGGATGACGTTGATAACTCCAAGCCAGGG
CCCAGCCGCCCAGGCCCTTCACCCCTGATCAATCAATACAGCCTAACAGTGGGACTGGAT
TTGGGGCCACACGACACTAAGTCTCTGCTTAAGTGTGTGGAATCGCTGTCCTTCATCGTG
CGTGATGCTGCCCACATCACACCTGACAACTTTGAGCTCTGCGTCAAGACTCTCCGGATC
TTTGTGGAGGCCAGTCTGAATGGCGGATGCAAGTCCCAGGAGAAACGTGGCAAGAGTCAC
AAATATGACAGCAAAGGGAACCGCTTCAAGAAGAAATCCAAAGAGGGATCAATGCTTCGC
CGGCCTCGAACCTCCAGCCAACATGCCTCTCGGGGCGGGCAGAGTGATGATGATGAGGAC
GAAGGCGTGCCTGCCAGCTACCATACGGTGTCTTTACAGGTCAGTCAGGACTTGCTAGAC
CTGATGCACACCCTGCACACGCGGGCAGCCTCTATCTACAGCTCATGGGCGGAGGAGCAA
CGCCACCTGGAGACAGGTGGCCAGAAGATTGAAGCTGATTCTCGCACCCTCTGGGCCCAC
TGCTGGTGCCCTTTACTGCAGGGTATTGCCTGCCTGTGCTGCGATGCCCGGCGCCAGGTA
CGGATGCAGGCACTGACCTATCTGCAGCGAGCACTACTTGTACATGATCTGCAAAAGCTA
GATGCCCTGGAATGGGAGTCCTGTTTTAACAAGGTGCTGTTTCCTCTACTTACCAAGCTC
TTGGAGAACATCAGCCCTGCAGATGTGGGTGGGATGGAGGAGACCCGGATGAGGGCTTCC
ACATTGCTCTCTAAGGTCTTCCTGCAGCACCTGTCTCCACTGCTGTCACTCTCTACCTTT
GCGGCCCTCTGGCTCACCATCTTGGACTTCATGGACAAGTACATGCACGCAGGCTCCAGC
GACTTACTGTCAGAGGCGATCCCTGAGTCTCTGAAGAACATGCTTCTGGTGATGGACACA
GCGGAGATTTTCCACAGTGCAGATGCACGGGGAGGCGGCCCCTCGGCCCTCTGGGAGATC
ACCTGGGAACGCATTGACTGTTTTCTCCCTCACCTACGAGATGAACTCTTCAAGCAGACC
GTCATCCAGGACCCCATGCCCATGGAGCCTCAAGGCCAAAAGCCTCTCGCCTCAGCCCAC
CTGACTTCCGCTGCTGGCGACACTAGGACACCTGGCCATCCACCGCCCCCAGAGATTCCA
TCTGAGCTGGGGGCCTGTGACTTTGAGAAGCCCGAGAGCCCCCGAGCCGCCAGCAGCAGC
TCCCCAGGATCACCAGTGGCCTCAAGCCCCAGCAGGCTGAGCCCCACCCCCGACGGGCCT
CCACCCTTGGCTCAGCCCCCACTGATCCTGCAGCCCTTGGCCTCCCCACTGCAGGTGGGC
GTGCCACCTATGACTCTGCCCATCATCCTCAACCCTGCGCTCATCGAGGCCACCTCACCA
GTGCCCCTCCTGGCCACACCCCGCCCCACAGATCCCATACCCACCTCTGAGGTCAAC

ORF Protein

Cloned ORF protein sequence for pFN21ASDA0248 Download

>KIAA0248  1859 aa
MVDKNIYIIQGEINIVVGAIKRNSRWSTHTPLDEERDPLLHSFGHLKEVLNSITELSEIE
PNVFLRPFLEVIRSEDTTGPITGLALTSVNKFLSYALIDPTHEGTAEGMENMADAVTHAR
FVGTDPASDEVVLMKILQVLRTLLLTPVGAHLTNESVCEIMQSCFRICFEMRLSELLRKS
AEHTLVDMVQLLFTRLPQFKEEPKNYVGTNMKKLKMRAGGMSDSSKWKKQKRSPRPPRHM
TKVTPGSELPTPNGTTLSSNLTGGMPFIDVPTPISSASSEAASAVVSPSTDSGLEFSSQT
TSKEDLTDLEQPGSPGYSTATEPGSSELGVPEQPDLQEGTHVEKSQSASVESIPEVLEEC
TSPADHSDSASVHDMDYVNPRGVRFTQSSQKEGTALVPYGLPCIRELFRFLISLTNPHDR
HNSEVMIHMGLHLLTVALESAPVAQCQTLLGLIKDEMCRHLFQLLSIERLNLYAASLRVC
FLLFESMREHLKFQMEMYIKKLMEIITVENPKMPYEMKEMALEAIVQLWRIPSFVTELYI
NYDCDYYCSNLFEELTKLLSKNAFPVSGQLYTTHLLSLDALLTVIDSTEAHCQAKVLNSL
TQQEKKETARPSCEIVDGTREASNTERTASDGKAVGMASDIPGLHLPGGGRLPPEHGKSG
CSDLEEAVDSGADKKFARKPPRFSCLLPDPRELIEIKNKKKLLITGTEQFNQKPKKGIQF
LQEKGLLTIPMDNTEVAQWLRENPRLDKKMIGEFVSDRKNIDLLESFVSTFSFQGLRLDE
ALRLYLEAFRLPGEAPVIQRLLEAFTERWMNCNGSPFANSDACFSLAYAVIMLNTDQHNH
NVRKQNAPMTLEEFRKNLKGVNGGKDFEQDILEDMYHAIKNEEIVMPEEQTGLVRENYVW
NVLLHRGATPEGIFLRVPTASYDLDLFTMTWGPTIAALSYVFDKSLEETIIQKAISGFRK
CAMISAHYGLSDVFDNLIISLCKFTALSSESIENLPSVFGSNPKAHIAAKTVFHLAHRHG
DILREGWKNIMEAMLQLFRAQLLPKAMIEVEDFVDPNGKISLQREETPSNRGESTVLSFV
SWLTLSGPEQSSVRGPSTENQEAKRVALECIKQCDPEKMITESKFLQLESLQELMKALVS
VTPDEETYDEEDAAFCLEMLLRIVLENRDRVGCVWQTVRDHLYHLCVQAQDFCFLVERAV
VGLLRLAIRLLRREEISAQVLLSLRILLLMKPSVLSRVSHQVAYGLHELLKTNAANIHSG
DDWATLFTLLECIGSGVKPPAALQATARADAPDAGAQSDSELPSYHQNDVSLDRGYTSDS
EVYTDHGRPGKIHRSATDADVVNSGWLVVGKDDVDNSKPGPSRPGPSPLINQYSLTVGLD
LGPHDTKSLLKCVESLSFIVRDAAHITPDNFELCVKTLRIFVEASLNGGCKSQEKRGKSH
KYDSKGNRFKKKSKEGSMLRRPRTSSQHASRGGQSDDDEDEGVPASYHTVSLQVSQDLLD
LMHTLHTRAASIYSSWAEEQRHLETGGQKIEADSRTLWAHCWCPLLQGIACLCCDARRQV
RMQALTYLQRALLVHDLQKLDALEWESCFNKVLFPLLTKLLENISPADVGGMEETRMRAS
TLLSKVFLQHLSPLLSLSTFAALWLTILDFMDKYMHAGSSDLLSEAIPESLKNMLLVMDT
AEIFHSADARGGGPSALWEITWERIDCFLPHLRDELFKQTVIQDPMPMEPQGQKPLASAH
LTSAAGDTRTPGHPPPPEIPSELGACDFEKPESPRAASSSSPGSPVASSPSRLSPTPDGP
PPLAQPPLILQPLASPLQVGVPPMTLPIILNPALIEATSPVPLLATPRPTDPIPTSEVN

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA0248 Download

>pFN21ASDA0248  10306 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGTGGATAAGAATAT
TTACATCATTCAAGGGGAGATTAACATTGTGGTTGGGGCCATCAAACGAAATTCCCGATG
GAGCACCCATACACCACTGGATGAAGAACGGGATCCTCTGCTGCATAGTTTCGGTCATCT
AAAGGAGGTTTTAAACAGTATAACAGAACTCTCAGAAATTGAGCCCAATGTATTCCTTCG
ACCTTTTCTGGAAGTGATTCGCTCTGAAGATACCACTGGCCCTATCACTGGACTGGCACT
CACCTCTGTCAACAAGTTCCTGTCCTATGCACTCATAGATCCCACCCATGAGGGCACAGC
AGAGGGCATGGAGAACATGGCAGATGCTGTCACCCATGCTCGTTTTGTGGGCACGGATCC
TGCCAGTGATGAAGTTGTCCTGATGAAAATCCTTCAGGTTCTACGGACTCTGCTGCTAAC
CCCAGTGGGTGCCCACCTAACCAATGAATCTGTGTGTGAGATTATGCAGTCTTGCTTCCG
GATCTGCTTTGAAATGAGGCTCAGTGAGTTATTGAGAAAATCCGCAGAGCACACTCTCGT
AGACATGGTGCAGCTGCTCTTCACAAGGTTACCTCAGTTTAAAGAAGAACCCAAGAACTA
TGTGGGGACCAACATGAAGAAGCTGAAAATGAGAGCCGGAGGCATGAGTGATTCATCCAA
ATGGAAGAAACAGAAGAGATCCCCTCGGCCCCCACGCCATATGACCAAAGTCACACCAGG
TTCAGAGCTGCCCACTCCCAATGGAACCACCTTATCATCTAACCTCACTGGTGGCATGCC
CTTCATTGATGTGCCCACTCCCATCTCCTCTGCAAGTTCAGAAGCTGCCTCAGCAGTGGT
CAGTCCCTCTACAGACAGTGGCCTGGAATTCTCCTCCCAAACCACTTCCAAGGAAGACCT
TACTGATCTAGAGCAACCTGGCTCTCCAGGGTACAGCACAGCTACAGAGCCTGGAAGCAG
TGAGCTAGGTGTTCCCGAGCAGCCTGACCTCCAGGAAGGGACCCATGTGGAAAAGTCCCA
GTCAGCATCTGTGGAGTCCATCCCTGAAGTGTTAGAGGAGTGCACGTCCCCTGCCGACCA
CTCTGACTCTGCCTCTGTCCATGACATGGATTACGTCAATCCCCGGGGCGTGCGCTTTAC
ACAGTCCTCCCAGAAAGAAGGCACAGCTTTGGTCCCCTATGGTCTTCCCTGCATCCGCGA
GCTCTTCCGCTTCCTCATCTCCCTCACCAATCCACACGACCGCCATAACTCAGAGGTTAT
GATTCACATGGGACTGCATTTGCTGACAGTGGCCCTTGAGTCAGCCCCTGTAGCCCAGTG
CCAGACCCTCTTGGGCCTCATCAAGGATGAGATGTGCCGTCACTTATTCCAGCTACTCAG
CATAGAGCGACTAAACCTTTATGCTGCTTCCCTGCGAGTATGCTTCCTACTGTTTGAGAG
CATGCGAGAGCACCTCAAGTTCCAAATGGAGATGTACATCAAAAAGCTTATGGAGATCAT
CACTGTGGAGAACCCCAAGATGCCTTATGAGATGAAGGAGATGGCACTGGAGGCCATTGT
GCAGCTCTGGCGCATCCCCAGCTTTGTCACAGAGCTCTACATCAACTATGATTGTGACTA
CTACTGTTCCAACCTCTTTGAGGAACTCACAAAGCTGCTGTCCAAGAATGCCTTCCCTGT
GTCTGGTCAACTCTATACAACACACCTACTATCTCTTGATGCCCTATTGACAGTGATTGA
CAGCACCGAGGCCCACTGCCAGGCTAAAGTCCTCAACAGCCTCACCCAGCAAGAGAAGAA
GGAGACAGCCAGACCAAGCTGTGAGATAGTAGATGGCACCCGAGAAGCTAGCAATACTGA
GAGAACTGCCAGCGATGGGAAAGCTGTAGGCATGGCCTCAGACATCCCAGGCCTGCATCT
GCCAGGTGGAGGGCGGCTGCCACCAGAACATGGGAAATCAGGATGCAGTGATCTGGAGGA
AGCTGTTGACTCTGGGGCTGACAAAAAGTTTGCCCGGAAGCCACCCCGATTTTCCTGTCT
CCTGCCAGATCCACGGGAACTAATTGAAATTAAAAACAAAAAGAAGCTGCTAATCACTGG
CACAGAGCAGTTCAATCAGAAACCAAAGAAGGGGATTCAGTTTCTGCAAGAGAAAGGCCT
CCTCACCATCCCAATGGACAACACAGAGGTTGCTCAGTGGCTCCGAGAGAACCCTCGGCT
GGACAAGAAGATGATTGGAGAGTTTGTGAGTGACCGCAAAAACATTGACCTGTTGGAGAG
CTTTGTGAGCACCTTCAGTTTTCAGGGTCTGCGACTGGACGAAGCCCTCCGCCTCTACCT
GGAAGCCTTCCGTTTGCCTGGGGAAGCACCAGTCATCCAGAGGTTGCTGGAGGCATTCAC
AGAGCGTTGGATGAATTGTAATGGCTCCCCATTTGCCAATAGCGATGCCTGCTTTTCCCT
GGCCTATGCTGTCATCATGCTTAATACTGACCAGCACAACCACAATGTTCGTAAACAGAA
TGCACCCATGACCCTGGAGGAGTTTCGCAAAAATCTGAAAGGTGTGAATGGAGGCAAGGA
CTTTGAGCAAGACATCCTGGAGGACATGTACCATGCCATCAAGAATGAGGAAATTGTAAT
GCCTGAGGAGCAGACAGGCTTGGTTCGGGAGAACTATGTGTGGAATGTGCTGCTTCATCG
AGGTGCCACCCCTGAGGGCATATTCCTGCGTGTGCCTACTGCCAGCTATGATCTTGACCT
CTTCACCATGACCTGGGGCCCCACTATTGCTGCTCTCTCTTATGTCTTTGACAAAAGCCT
TGAGGAGACAATCATCCAGAAAGCCATCTCAGGCTTCAGGAAGTGCGCCATGATCTCCGC
CCACTATGGCCTCAGCGATGTGTTTGACAATCTCATCATCTCTCTATGCAAATTCACAGC
TCTCAGCAGTGAGTCTATTGAGAACCTGCCCAGTGTATTTGGAAGCAACCCTAAAGCCCA
TATTGCAGCCAAGACAGTATTCCATTTGGCCCATCGTCATGGTGACATCCTGCGGGAGGG
CTGGAAGAATATCATGGAGGCCATGCTGCAGCTCTTCCGAGCCCAACTACTGCCCAAGGC
TATGATAGAGGTAGAAGATTTCGTGGATCCCAATGGCAAGATCTCTCTACAGCGGGAAGA
GACACCATCAAACCGAGGAGAGTCAACAGTGCTGAGCTTTGTGAGCTGGCTAACACTGAG
TGGTCCTGAGCAGTCTAGTGTTCGGGGCCCATCCACTGAAAACCAAGAGGCCAAGAGAGT
GGCCTTAGAGTGTATAAAGCAATGTGACCCAGAAAAAATGATCACAGAAAGCAAGTTCCT
CCAGCTGGAGTCACTACAGGAGCTCATGAAGGCTCTGGTCTCAGTGACACCAGATGAAGA
GACATATGATGAGGAAGATGCTGCTTTCTGCCTAGAGATGCTGCTAAGGATTGTGTTGGA
GAACAGGGATCGTGTGGGCTGTGTGTGGCAGACTGTTCGAGACCATCTATACCACCTCTG
TGTTCAGGCACAAGATTTCTGCTTCCTTGTGGAGCGGGCAGTGGTGGGGTTGCTACGCCT
GGCCATTCGGCTTCTCCGGAGAGAAGAGATCAGTGCTCAGGTGCTGCTCTCCCTGCGCAT
TTTGCTACTGATGAAGCCCAGTGTGCTATCCCGAGTCAGCCACCAGGTTGCGTATGGGCT
CCATGAACTCCTGAAGACCAATGCAGCCAACATCCACTCAGGTGATGACTGGGCCACACT
CTTCACACTGCTGGAGTGCATCGGCTCAGGTGTGAAGCCTCCAGCTGCTCTGCAGGCCAC
AGCCAGGGCAGATGCACCTGATGCCGGGGCCCAGTCAGATAGTGAGCTCCCATCCTACCA
TCAGAATGACGTGAGCCTGGATCGAGGGTACACTTCCGACTCAGAGGTCTACACTGACCA
TGGCAGGCCGGGCAAGATACACCGATCAGCCACAGATGCCGATGTGGTCAACAGTGGTTG
GTTAGTGGTTGGGAAGGATGACGTTGATAACTCCAAGCCAGGGCCCAGCCGCCCAGGCCC
TTCACCCCTGATCAATCAATACAGCCTAACAGTGGGACTGGATTTGGGGCCACACGACAC
TAAGTCTCTGCTTAAGTGTGTGGAATCGCTGTCCTTCATCGTGCGTGATGCTGCCCACAT
CACACCTGACAACTTTGAGCTCTGCGTCAAGACTCTCCGGATCTTTGTGGAGGCCAGTCT
GAATGGCGGATGCAAGTCCCAGGAGAAACGTGGCAAGAGTCACAAATATGACAGCAAAGG
GAACCGCTTCAAGAAGAAATCCAAAGAGGGATCAATGCTTCGCCGGCCTCGAACCTCCAG
CCAACATGCCTCTCGGGGCGGGCAGAGTGATGATGATGAGGACGAAGGCGTGCCTGCCAG
CTACCATACGGTGTCTTTACAGGTCAGTCAGGACTTGCTAGACCTGATGCACACCCTGCA
CACGCGGGCAGCCTCTATCTACAGCTCATGGGCGGAGGAGCAACGCCACCTGGAGACAGG
TGGCCAGAAGATTGAAGCTGATTCTCGCACCCTCTGGGCCCACTGCTGGTGCCCTTTACT
GCAGGGTATTGCCTGCCTGTGCTGCGATGCCCGGCGCCAGGTACGGATGCAGGCACTGAC
CTATCTGCAGCGAGCACTACTTGTACATGATCTGCAAAAGCTAGATGCCCTGGAATGGGA
GTCCTGTTTTAACAAGGTGCTGTTTCCTCTACTTACCAAGCTCTTGGAGAACATCAGCCC
TGCAGATGTGGGTGGGATGGAGGAGACCCGGATGAGGGCTTCCACATTGCTCTCTAAGGT
CTTCCTGCAGCACCTGTCTCCACTGCTGTCACTCTCTACCTTTGCGGCCCTCTGGCTCAC
CATCTTGGACTTCATGGACAAGTACATGCACGCAGGCTCCAGCGACTTACTGTCAGAGGC
GATCCCTGAGTCTCTGAAGAACATGCTTCTGGTGATGGACACAGCGGAGATTTTCCACAG
TGCAGATGCACGGGGAGGCGGCCCCTCGGCCCTCTGGGAGATCACCTGGGAACGCATTGA
CTGTTTTCTCCCTCACCTACGAGATGAACTCTTCAAGCAGACCGTCATCCAGGACCCCAT
GCCCATGGAGCCTCAAGGCCAAAAGCCTCTCGCCTCAGCCCACCTGACTTCCGCTGCTGG
CGACACTAGGACACCTGGCCATCCACCGCCCCCAGAGATTCCATCTGAGCTGGGGGCCTG
TGACTTTGAGAAGCCCGAGAGCCCCCGAGCCGCCAGCAGCAGCTCCCCAGGATCACCAGT
GGCCTCAAGCCCCAGCAGGCTGAGCCCCACCCCCGACGGGCCTCCACCCTTGGCTCAGCC
CCCACTGATCCTGCAGCCCTTGGCCTCCCCACTGCAGGTGGGCGTGCCACCTATGACTCT
GCCCATCATCCTCAACCCTGCGCTCATCGAGGCCACCTCACCAGTGCCCCTCCTGGCCAC
ACCCCGCCCCACAGATCCCATACCCACCTCTGAGGTCAACTACGTAGTTTAAACGAATTC
GGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATCCGGCTG
CTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAACTAGCAT
AACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTTGGACAA
ACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCTATTGCT
TTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATTCATTTT
ATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTCTACAAA
TGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTCCAGATA
GCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTCTACGTA
ATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGT
TTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATG
CTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATT
CCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTA
AAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGC
GGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTCAAA
GTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGC
CGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTT
ACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACT
GCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCAC
AACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATA
CCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTA
TTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCG
GATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGAT
AAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGT
AAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGA
AATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAAATGACC
GACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAG
AATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC
GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACA
AAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGT
TTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACC
TGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATC
TCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC
CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACT
TATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTG
CTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTATTTGGTA
TCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCA
AACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAA
AAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACG
AAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCC
TTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGGTGAAAC
CATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCTCTGGTT
GCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCGGGTGGT
TTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCGGTGCGT
ACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAGATAAAC
CGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT