GPR83 ORF clone FHC00859

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Halotag ORF Clone FHC00859

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Clone info.

Species

human

Clone Name

pFN21ASDA1540 Sequence (with vector) Restriction Map

Product ID

FHC00859

Flexi Clone PID

FXC00859

ORF Sequence

DNA sequence (1269 bp)
Protein sequence (423 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384231]

Gene

KIAA1540

Symbol

GPR83

Alias : GIR, GPR72

Description

G protein-coupled receptor 83

Original Clone ID

fj00652

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1269 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 423 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS8297.1 5.2e-135 99.8 GPR83 CCDS81616.1 2.4e-79 89.8 GPR83 CCDS7606.1 7.7e-27 36.3 PRLHR CCDS1958.1 1.6e-26 34.3 TACR1 CCDS3664.1 7.8e-26 33.8 TACR3 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000276 73 97 PR00237 GPCR IPR000611 98 110 PR01012 Neuropeptide Y receptor IPR000276 106 127 PR00237 GPCR IPR000611 128 140 PR01012 Neuropeptide Y receptor IPR000276 151 173 PR00237 GPCR IPR000611 151 166 PR01012 Neuropeptide Y receptor IPR000276 185 206 PR00237 GPCR IPR000276 240 263 PR00237 GPCR IPR000276 290 314 PR00237 GPCR IPR000276 327 353 PR00237 GPCR IPR000611 332 341 PR01012 Neuropeptide Y receptor IPR000611 343 356 PR01012 Neuropeptide Y receptor HMMPfam IPR000276 88 345 PF00001 GPCR ProfileScan IPR017452 88 345 PS50262 GPCR
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 1 MVPHLLLLCLLPLVRATEPH 20 SECONDARY 20 2 72 ALLIVAYSFIIVFSLFGNVLVCH 94 PRIMARY 23 3 114 AVADIMITLLNTPFTLVRFVNST 136 SECONDARY 23 4 186 GVIYIAVIWTMATFFSLPHAICQ 208 SECONDARY 23 5 240 DLATFILLYILPLLIISVAYARV 262 PRIMARY 23 6 294 LMLVVVLFALCWFPLNCYVLLLS 316 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_057624 423 605569 99.8 100.0 NP_001317274 381 605569 89.8 100.0 NP_001048 398 162321 31.4 80.1 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1540 Download

>KIAA1540  1269 bp
ATGGTCCCTCACCTCTTGCTGCTCTGTCTCCTCCCCTTGGTGCGAGCCACCGAGCCCCAC
GAGGGCCGGGCCGACGAGCAGAGCGCGGAGGCGGCCCTGGCCGTGCCCAATGCCTCGCAC
TTCTTCTCTTGGAACAACTACACCTTCTCCGACTGGCAGAACTTTGTGGGCAGGAGGCGC
TACGGCGCTGAGTCCCAGAACCCCACGGTGAAAGCCCTGCTCATTGTGGCTTACTCCTTC
ATCATTGTCTTCTCACTCTTTGGCAACGTCCTGGTCTGTCATGTCATCTTCAAGAACCAG
CGAATGCACTCGGCCACCAGCCTCTTCATCGTCAACCTGGCAGTTGCCGACATAATGATC
ACGCTGCTCAACACCCCCTTCACTTTGGTTCGCTTTGTGAACAGCACATGGATATTTGGG
AAGGGCATGTGCCATGTCAGCCGCTTTGCCCAGTACTGCTCACTGCACGTCTCAGCACTG
ACACTGACAGCCATTGCGGTGGATCGCCACCAGGTCATCATGCACCCCTTGAAACCCCGG
ATCTCAATCACAAAGGGTGTCATCTACATCGCTGTCATCTGGACCATGGCTACGTTCTTT
TCACTCCCACATGCTATCTGCCAGAAATTATTTACCTTCAAATACAGTGAGGACATTGTG
CGCTCCCTCTGCCTGCCAGACTTCCCTGAGCCAGCTGACCTCTTCTGGAAGTACCTGGAC
TTGGCCACCTTCATCCTGCTCTACATCCTGCCCCTCCTCATCATCTCTGTGGCCTACGCT
CGTGTGGCCAAGAAACTGTGGCTGTGTAATATGATTGGCGATGTGACCACAGAGCAGTAC
TTTGCCCTGCGGCGCAAAAAGAAGAAGACCATCAAGATGTTGATGCTGGTGGTAGTCCTC
TTTGCCCTCTGCTGGTTCCCCCTCAACTGCTACGTCCTCCTCCTGTCCAGCAAGGTCATC
CGCACCAACAATGCCCTCTACTTTGCCTTCCACTGGTTTGCCATGAGCAGCACCTGCTAT
AACCCCTTCATATACTGCTGGCTGAACGAGAACTTCAGGATTGAGCTAAAGGCATTACTG
AGCATGTGTCAAAGACCTCCCAAGCCTCAGGAGGACAGGCAACCCTCCCCAGTTCCTTCC
TTCAGGGTGGCCTGGACAGAGAAGAATGATGGCCAGAGGGCTCCCCTTGCCAATAACCTC
CTGCCCACCTCCCAACTCCAGTCTGGGAAGACAGACCTGTCATCTGTGGAACCCATTGTG
ACGATGAGT

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1540 Download

>KIAA1540  423 aa
MVPHLLLLCLLPLVRATEPHEGRADEQSAEAALAVPNASHFFSWNNYTFSDWQNFVGRRR
YGAESQNPTVKALLIVAYSFIIVFSLFGNVLVCHVIFKNQRMHSATSLFIVNLAVADIMI
TLLNTPFTLVRFVNSTWIFGKGMCHVSRFAQYCSLHVSALTLTAIAVDRHQVIMHPLKPR
ISITKGVIYIAVIWTMATFFSLPHAICQKLFTFKYSEDIVRSLCLPDFPEPADLFWKYLD
LATFILLYILPLLIISVAYARVAKKLWLCNMIGDVTTEQYFALRRKKKKTIKMLMLVVVL
FALCWFPLNCYVLLLSSKVIRTNNALYFAFHWFAMSSTCYNPFIYCWLNENFRIELKALL
SMCQRPPKPQEDRQPSPVPSFRVAWTEKNDGQRAPLANNLLPTSQLQSGKTDLSSVEPIV
TMS

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1540 Download

>pFN21ASDA1540  5998 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGTCCCTCACCTCTT
GCTGCTCTGTCTCCTCCCCTTGGTGCGAGCCACCGAGCCCCACGAGGGCCGGGCCGACGA
GCAGAGCGCGGAGGCGGCCCTGGCCGTGCCCAATGCCTCGCACTTCTTCTCTTGGAACAA
CTACACCTTCTCCGACTGGCAGAACTTTGTGGGCAGGAGGCGCTACGGCGCTGAGTCCCA
GAACCCCACGGTGAAAGCCCTGCTCATTGTGGCTTACTCCTTCATCATTGTCTTCTCACT
CTTTGGCAACGTCCTGGTCTGTCATGTCATCTTCAAGAACCAGCGAATGCACTCGGCCAC
CAGCCTCTTCATCGTCAACCTGGCAGTTGCCGACATAATGATCACGCTGCTCAACACCCC
CTTCACTTTGGTTCGCTTTGTGAACAGCACATGGATATTTGGGAAGGGCATGTGCCATGT
CAGCCGCTTTGCCCAGTACTGCTCACTGCACGTCTCAGCACTGACACTGACAGCCATTGC
GGTGGATCGCCACCAGGTCATCATGCACCCCTTGAAACCCCGGATCTCAATCACAAAGGG
TGTCATCTACATCGCTGTCATCTGGACCATGGCTACGTTCTTTTCACTCCCACATGCTAT
CTGCCAGAAATTATTTACCTTCAAATACAGTGAGGACATTGTGCGCTCCCTCTGCCTGCC
AGACTTCCCTGAGCCAGCTGACCTCTTCTGGAAGTACCTGGACTTGGCCACCTTCATCCT
GCTCTACATCCTGCCCCTCCTCATCATCTCTGTGGCCTACGCTCGTGTGGCCAAGAAACT
GTGGCTGTGTAATATGATTGGCGATGTGACCACAGAGCAGTACTTTGCCCTGCGGCGCAA
AAAGAAGAAGACCATCAAGATGTTGATGCTGGTGGTAGTCCTCTTTGCCCTCTGCTGGTT
CCCCCTCAACTGCTACGTCCTCCTCCTGTCCAGCAAGGTCATCCGCACCAACAATGCCCT
CTACTTTGCCTTCCACTGGTTTGCCATGAGCAGCACCTGCTATAACCCCTTCATATACTG
CTGGCTGAACGAGAACTTCAGGATTGAGCTAAAGGCATTACTGAGCATGTGTCAAAGACC
TCCCAAGCCTCAGGAGGACAGGCAACCCTCCCCAGTTCCTTCCTTCAGGGTGGCCTGGAC
AGAGAAGAATGATGGCCAGAGGGCTCCCCTTGCCAATAACCTCCTGCCCACCTCCCAACT
CCAGTCTGGGAAGACAGACCTGTCATCTGTGGAACCCATTGTGACGATGAGTTACGTAGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT