SH2B1 ORF clone FHC01158

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone FHC01158

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Clone info.

Species

human

Clone Name

pFN21AA1299 Sequence (with vector) Restriction Map

Product ID

FHC01158

Flexi Clone PID

FXC01158

ORF Sequence

DNA sequence (2013 bp)
Protein sequence (671 aa)

Linker Type

Flexi type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB385445]

Gene

KIAA1299

Symbol

SH2B1

Alias : PSM, SH2B

Description

SH2B adaptor protein 1, transcript variant 2

Original Clone ID

fg04370

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 2013 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 671 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS32424.1 1.4e-177 100.0 SH2B1 CCDS53997.1 2.6e-166 94.3 SH2B1 CCDS53996.1 7.7e-166 99.2 SH2B1 CCDS76851.1 9.6e-87 100.0 SH2B1 CCDS78264.1 1.4e-17 41.1 SH2B2 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition FPrintScan IPR000980 527 541 PR00401 SH2 motif IPR000980 549 559 PR00401 SH2 motif IPR000980 561 572 PR00401 SH2 motif IPR000980 593 607 PR00401 SH2 motif HMMPfam IPR015012 24 81 PF08916 Phenylalanine zipper IPR001849 282 376 PF00169 Pleckstrin homology domain IPR000980 527 604 PF00017 SH2 motif HMMSmart IPR001849 247 378 SM00233 Pleckstrin homology domain IPR000980 525 610 SM00252 SH2 motif ProfileScan IPR000980 527 625 PS50001 SH2 motif
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) NP_001139284 671 608937 100.0 100.0 NP_056318 671 608937 100.0 100.0 NP_001139268 671 608937 100.0 100.0 NP_001139269 683 608937 94.3 99.9 NP_001139267 756 608937 99.2 95.4 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21AA1299 Download

>KIAA1299  2013 bp
ATGAATGGTGCCCCTTCCCCAGAGGACGGGGCCTCCCCCTCGTCTCCCCCGCTGCCCCCA
CCCCCGCCCCCTAGTTGGCGGGAGTTCTGTGAGTCCCACGCCCGGGCTGCGGCTCTGGAC
TTTGCCCGCCGTTTTCGCCTCTACCTGGCCTCCCACCCCCAATATGCGGGGCCCGGGGCC
GAGGCTGCCTTCTCCCGCCGTTTTGCTGAGCTCTTCCTGCAGCACTTTGAAGCCGAGGTG
GCCCGGGCCTCTGGCTCCCTGTCGCCACCCATCCTGGCTCCCCTGAGCCCTGGTGCGGAG
ATTTCGCCACATGACCTGTCCCTTGAGAGCTGCAGGGTGGGTGGGCCCCTGGCTGTGCTG
GGCCCTTCTCGATCATCTGAGGACCTGGCCGGCCCCCTCCCTTCCTCAGTCTCTTCCTCC
TCTACAACCTCCTCCAAGCCGAAGCTCAAGAAGCGCTTTTCCCTGCGTTCAGTGGGTCGC
TCTGTCCGAGGCTCAGTCCGTGGCATCCTGCAGTGGCGGGGGACCGTTGACCCTCCCTCC
TCCGCTGGGCCCCTGGAGACCTCGTCAGGCCCCCCAGTCTTAGGTGGAAACAGCAACTCC
AACTCCTCTGGCGGGGCTGGGACCGTTGGTAGGGGACTGGTCAGTGATGGAACGTCCCCT
GGGGAAAGATGGACTCACCGTTTTGAGAGGCTGAGACTCAGTCGGGGAGGGGGCGCCTTG
AAGGATGGAGCAGGGATGGTGCAGAGGGAAGAGCTGCTGAGTTTCATGGGGGCTGAGGAG
GCAGCCCCTGACCCAGCCGGAGTGGGCCGGGGAGGAGGGGTGGCTGGGCCTCCTTCAGGG
GGAGGAGGGCAGCCTCAGTGGCAGAAGTGTCGCCTGCTGCTTCGAAGTGAAGGAGAAGGA
GGAGGAGGAAGTCGCCTGGAGTTCTTTGTACCACCCAAGGCCTCTCGGCCCCGACTCAGC
ATCCCCTGCTCTTCTATCACAGACGTCCGGACAACCACAGCCCTGGAGATGCCTGACCGG
GAGAACACGTTTGTGGTTAAGGTGGAAGGTCCATCCGAGTATATCATGGAGACAGTGGAT
GCCCAGCATGTGAAGGCCTGGGTGTCTGACATCCAAGAATGCCTGAGCCCAGGACCCTGC
CCTGCTACCAGTCCCCGCCCCATGACCCTCCCTCTGGCCCCTGGGACCTCATTCCTTACA
AGGGAGAACACAGACAGCCTGGAGCTGTCCTGCCTGAATCACTCGGAGAGTCTACCCAGC
CAGGACCTGCTGCTTGGACCCAGCGAGAGCAATGACCGCCTGTCGCAGGGGGCATATGGG
GGCCTCTCAGACCGCCCCTCGGCATCCATCTCCCCCAGCTCTGCCTCCATTGCCGCCTCC
CATTTTGACTCGATGGAACTGCTTCCCCCAGAGTTGCCCCCCCGCATCCCCATTGAAGAG
GGACCCCCAACAGGGACAGTTCATCCCCTCTCAGCCCCCTACCCTCCCTTGGACACTCCG
GAAACAGCCACAGGGTCCTTCCTGTTCCAGGGGGAGCCAGAGGGCGGTGAGGGGGACCAG
CCCCTCTCAGGGTATCCTTGGTTCCACGGGATGCTCTCTCGGCTCAAGGCTGCACAGTTG
GTGCTGACTGGCGGCACTGGCTCCCACGGTGTCTTCCTGGTGCGCCAGAGTGAGACAAGG
CGGGGTGAATACGTCCTCACCTTCAACTTCCAGGGCAAGGCCAAGCACCTGCGTTTGTCG
CTGAACGAGGAGGGTCAGTGCCGGGTCCAGCACCTGTGGTTCCAGTCCATTTTCGATATG
CTCGAGCACTTCCGGGTGCACCCCATCCCTTTGGAGTCGGGAGGCTCCAGTGATGTTGTC
CTTGTCAGCTATGTCCCATCCTCCCAGCGACAGCAGGGCCGGGAGCAGGCTGGGAGCCAT
GCGGGGGTGTGCGAGGGAGATGGATGCCACCCCGATGCCTCCTGCACCCTCATGCCCTTC
GGAGCGAGTGACTGTGTAACCGACCACCTCCCA

ORF Protein

Cloned ORF protein sequence for pFN21AA1299 Download

>KIAA1299  671 aa
MNGAPSPEDGASPSSPPLPPPPPPSWREFCESHARAAALDFARRFRLYLASHPQYAGPGA
EAAFSRRFAELFLQHFEAEVARASGSLSPPILAPLSPGAEISPHDLSLESCRVGGPLAVL
GPSRSSEDLAGPLPSSVSSSSTTSSKPKLKKRFSLRSVGRSVRGSVRGILQWRGTVDPPS
SAGPLETSSGPPVLGGNSNSNSSGGAGTVGRGLVSDGTSPGERWTHRFERLRLSRGGGAL
KDGAGMVQREELLSFMGAEEAAPDPAGVGRGGGVAGPPSGGGGQPQWQKCRLLLRSEGEG
GGGSRLEFFVPPKASRPRLSIPCSSITDVRTTTALEMPDRENTFVVKVEGPSEYIMETVD
AQHVKAWVSDIQECLSPGPCPATSPRPMTLPLAPGTSFLTRENTDSLELSCLNHSESLPS
QDLLLGPSESNDRLSQGAYGGLSDRPSASISPSSASIAASHFDSMELLPPELPPRIPIEE
GPPTGTVHPLSAPYPPLDTPETATGSFLFQGEPEGGEGDQPLSGYPWFHGMLSRLKAAQL
VLTGGTGSHGVFLVRQSETRRGEYVLTFNFQGKAKHLRLSLNEEGQCRVQHLWFQSIFDM
LEHFRVHPIPLESGGSSDVVLVSYVPSSQRQQGREQAGSHAGVCEGDGCHPDASCTLMPF
GASDCVTDHLP

ORF+Vector

Nucleotide Sequence (with vector) for pFN21AA1299 Download

>pFN21AA1299  6718 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCCATGAATGGTGCCCCTTCCCCAGAGGACGGGGCCTC
CCCCTCGTCTCCCCCGCTGCCCCCACCCCCGCCCCCTAGTTGGCGGGAGTTCTGTGAGTC
CCACGCCCGGGCTGCGGCTCTGGACTTTGCCCGCCGTTTTCGCCTCTACCTGGCCTCCCA
CCCCCAATATGCGGGGCCCGGGGCCGAGGCTGCCTTCTCCCGCCGTTTTGCTGAGCTCTT
CCTGCAGCACTTTGAAGCCGAGGTGGCCCGGGCCTCTGGCTCCCTGTCGCCACCCATCCT
GGCTCCCCTGAGCCCTGGTGCGGAGATTTCGCCACATGACCTGTCCCTTGAGAGCTGCAG
GGTGGGTGGGCCCCTGGCTGTGCTGGGCCCTTCTCGATCATCTGAGGACCTGGCCGGCCC
CCTCCCTTCCTCAGTCTCTTCCTCCTCTACAACCTCCTCCAAGCCGAAGCTCAAGAAGCG
CTTTTCCCTGCGTTCAGTGGGTCGCTCTGTCCGAGGCTCAGTCCGTGGCATCCTGCAGTG
GCGGGGGACCGTTGACCCTCCCTCCTCCGCTGGGCCCCTGGAGACCTCGTCAGGCCCCCC
AGTCTTAGGTGGAAACAGCAACTCCAACTCCTCTGGCGGGGCTGGGACCGTTGGTAGGGG
ACTGGTCAGTGATGGAACGTCCCCTGGGGAAAGATGGACTCACCGTTTTGAGAGGCTGAG
ACTCAGTCGGGGAGGGGGCGCCTTGAAGGATGGAGCAGGGATGGTGCAGAGGGAAGAGCT
GCTGAGTTTCATGGGGGCTGAGGAGGCAGCCCCTGACCCAGCCGGAGTGGGCCGGGGAGG
AGGGGTGGCTGGGCCTCCTTCAGGGGGAGGAGGGCAGCCTCAGTGGCAGAAGTGTCGCCT
GCTGCTTCGAAGTGAAGGAGAAGGAGGAGGAGGAAGTCGCCTGGAGTTCTTTGTACCACC
CAAGGCCTCTCGGCCCCGACTCAGCATCCCCTGCTCTTCTATCACAGACGTCCGGACAAC
CACAGCCCTGGAGATGCCTGACCGGGAGAACACGTTTGTGGTTAAGGTGGAAGGTCCATC
CGAGTATATCATGGAGACAGTGGATGCCCAGCATGTGAAGGCCTGGGTGTCTGACATCCA
AGAATGCCTGAGCCCAGGACCCTGCCCTGCTACCAGTCCCCGCCCCATGACCCTCCCTCT
GGCCCCTGGGACCTCATTCCTTACAAGGGAGAACACAGACAGCCTGGAGCTGTCCTGCCT
GAATCACTCGGAGAGTCTACCCAGCCAGGACCTGCTGCTTGGACCCAGCGAGAGCAATGA
CCGCCTGTCGCAGGGGGCATATGGGGGCCTCTCAGACCGCCCCTCGGCATCCATCTCCCC
CAGCTCTGCCTCCATTGCCGCCTCCCATTTTGACTCGATGGAACTGCTTCCCCCAGAGTT
GCCCCCCCGCATCCCCATTGAAGAGGGACCCCCAACAGGGACAGTTCATCCCCTCTCAGC
CCCCTACCCTCCCTTGGACACTCCGGAAACAGCCACAGGGTCCTTCCTGTTCCAGGGGGA
GCCAGAGGGCGGTGAGGGGGACCAGCCCCTCTCAGGGTATCCTTGGTTCCACGGGATGCT
CTCTCGGCTCAAGGCTGCACAGTTGGTGCTGACTGGCGGCACTGGCTCCCACGGTGTCTT
CCTGGTGCGCCAGAGTGAGACAAGGCGGGGTGAATACGTCCTCACCTTCAACTTCCAGGG
CAAGGCCAAGCACCTGCGTTTGTCGCTGAACGAGGAGGGTCAGTGCCGGGTCCAGCACCT
GTGGTTCCAGTCCATTTTCGATATGCTCGAGCACTTCCGGGTGCACCCCATCCCTTTGGA
GTCGGGAGGCTCCAGTGATGTTGTCCTTGTCAGCTATGTCCCATCCTCCCAGCGACAGCA
GGGCCGGGAGCAGGCTGGGAGCCATGCGGGGGTGTGCGAGGGAGATGGATGCCACCCCGA
TGCCTCCTGCACCCTCATGCCCTTCGGAGCGAGTGACTGTGTAACCGACCACCTCCCAGT
TTAAACGAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAG
CTGATCCGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGC
AATAACTAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGAT
GAGTTTGGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGT
GATGCTATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAAT
TGCATTCATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAA
AACCTCTACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCC
CTTGTCCAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTG
GCTTTCTACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGA
ACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAA
CCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGT
GTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACG
CTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTG
GATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATG
AGCACTTTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAG
CAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACA
GAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATG
AGTGATAACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACC
GCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTG
AATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACG
TTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGAC
TGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGG
TTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTG
GGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACT
ATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA
TTCGAAATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATAC
GGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAA
AGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTG
ACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAA
GATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGC
TTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCAC
GCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAAC
CCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGG
TAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGT
ATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGA
CAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCT
CTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGA
TTACGCGCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACG
CTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCT
TCACCTAGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTG
GGATGGTGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTG
TACCCTCTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGT
TTTTCGGGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTT
TTAGCGGTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCC
ATCGAGATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT