VANGL2 ORF clone pFN21ASDA1215

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KOP HaloTag ORF List Flexi search

Halotag ORF Clone pFN21ASDA1215

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Clone info.

Species

human

Clone Name

pFN21ASDA1215 Sequence (with vector) Restriction Map

Product ID

FHC00769

Flexi Clone PID

FXC00769

ORF Sequence

DNA sequence (1563 bp)
Protein sequence (521 aa)

Linker Type

Flexi_RBS type

more Linker info

Vector Type

pFN21A Vector sequence

Restriction Map

Cloning Site

SgfI-PmeI

GenBank Accession

ORF sequence is derived from [AB384142]

Gene

KIAA1215

Symbol

VANGL2

Alias : LPP1, LTAP, STB1, STBM, STBM1

Description

VANGL planar cell polarity protein 2

Original Clone ID

fh01854

Quality control data of this clone

Description

Characterization of the cloned ORF

5'-end(FN21F1)

3'-end(F1KR)

Agarose gel electrophoretic image

5'/3'-end sequences (chromatogram)

N-terminal HaloTag-fusions expressed in HEK293 visualized by HaloTag TMR ligand

Cell image

SDS-PAGE

Features of the cloned ORF sequence

Description

Length: 1563 bp
Restriction map A Restriction map B
Genomic Structure

Features of the protein sequence

Description

Length: 521 aa
Result of homology search against CCDS protein database ( FASTA output, Multiple alignment) Entry Exp ID% symbol CCDS30915.1 5.8e-193 100.0 VANGL2 CCDS883.1 1.7e-142 72.8 VANGL1 CCDS53350.1 2e-142 73.0 VANGL1 The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI) [ zoom out (x2) ]
Result of InterProScan Search method interpro_ID From To Entry Definition HMMPfam IPR009539 23 521 PF06638 Strabismus
Prediction of transmembrane (TM) segments Method No. N terminal transmembrane region C terminal type length SOSUI2 1 110 AAGATLALLSFLTPLAFLLLPPL 132 PRIMARY 23 2 142 GTACEGLFISVAFKLLILLLGSW 164 PRIMARY 23 3 179 FVLRALLMVLVFLLVVSYWLFYG 201 PRIMARY 23 4 218 FAVSLVDALLFVHYLAVVLLELR 240 PRIMARY 23
Result of homology search against disease genes in OMIM database(FASTA output, Multiple alignment) H_id H_len (aa) OMIM_No. ID (%) Q_ali/Q_len (%) XP_011508106 521 182940,600533 100.0 100.0 XP_005245414 521 182940,600533 100.0 100.0 NP_065068 521 182940,600533 100.0 100.0 NP_620409 524 182940,600145,610132 72.8 100.0 NP_001165883 524 182940,600145,610132 72.8 100.0 Inquiries or Suggestions ? Send a message to flexiclone AT kazusagt.com

ORF DNA

Cloned ORF Nucleotide sequence for pFN21ASDA1215 Download

>KIAA1215  1563 bp
ATGGACACCGAGTCCCAGTACTCGGGCTATTCCTACAAGTCGGGCCACTCCCGCAGCTCC
CGCAAGCACAGGGACCGCCGGGACCGACACCGCTCTAAGAGTCGAGATGGGGGCCGAGGG
GACAAGTCGGTGACAATCCAGGCTCCCGGGGAGCCCCTGCTGGACAATGAGTCCACACGA
GGGGATGAGCGGGATGACAACTGGGGGGAAACGACGACAGTAGTAACGGGCACCTCAGAG
CACAGCATCTCCCATGATGACCTCACACGCATCGCCAAGGACATGGAGGACAGTGTCCCT
CTGGACTGCTCCCGTCACCTGGGTGTGGCAGCGGGGGCCACCCTGGCACTGCTGTCTTTC
CTCACGCCTCTGGCCTTCCTGCTGCTGCCCCCACTGCTGTGGCGGGAGGAGCTGGAGCCT
TGCGGGACGGCCTGCGAGGGCCTCTTCATCTCTGTCGCCTTCAAGCTGCTCATCCTGCTA
CTGGGCAGCTGGGCTCTGTTCTTCCGCCGGCCCAAGGCCTCGCTGCCCCGCGTCTTTGTG
CTGCGTGCCCTGCTTATGGTGCTGGTTTTCCTGCTCGTGGTCTCCTACTGGCTCTTCTAT
GGTGTGCGCATCCTGGATGCTCGGGAGCGCAGCTACCAGGGCGTGGTGCAGTTCGCCGTG
TCGCTGGTGGACGCCCTTCTTTTCGTGCACTACCTGGCCGTGGTCCTGCTGGAGCTGCGC
CAGCTCCAGCCTCAGTTCACGCTCAAGGTCGTGCGCTCCACCGACGGCGCCAGCCGCTTC
TACAACGTTGGCCATCTCAGCATCCAGCGCGTGGCAGTGTGGATCCTGGAGAAGTATTAC
CATGACTTCCCTGTCTACAACCCTGCCCTCCTCAACCTGCCCAAGTCCGTCCTGGCCAAG
AAAGTGTCTGGCTTCAAGGTGTATTCCCTCGGAGAGGAAAACAGCACCAACAACTCCACT
GGCCAGTCTCGGGCTGTGATTGCAGCGGCAGCTCGGAGGCGGGACAACAGTCACAATGAG
TACTACTATGAGGAGGCTGAGCATGAGCGAAGGGTGCGCAAGAGGAGGGCCAGGCTTGTA
GTGGCGGTGGAGGAGGCCTTCACTCACATTAAGCGGCTGCAGGAAGAGGAGCAGAAAAAC
CCCAGGGAGGTGATGGACCCCCGGGAGGCAGCCCAAGCCATCTTTGCATCCATGGCCCGT
GCCATGCAGAAGTACCTTCGGACCACCAAGCAGCAGCCCTACCACACCATGGAGAGCATC
CTGCAGCACCTTGAATTCTGCATCACGCATGACATGACGCCCAAGGCCTTCTTGGAGCGA
TACTTGGCGGCTGGACCTACCATCCAGTACCACAAGGAACGCTGGCTGGCCAAACAGTGG
ACATTGGTGAGCGAGGAGCCGGTGACCAACGGCCTCAAGGATGGCATCGTTTTCCTCTTA
AAACGCCAGGACTTCAGCCTGGTGGTCAGCACCAAGAAGGTCCCATTCTTCAAACTCTCC
GAGGAATTTGTGGATCCCAAGTCACACAAGTTTGTCATGAGGCTGCAGTCTGAGACCTCA
GTG

ORF Protein

Cloned ORF protein sequence for pFN21ASDA1215 Download

>KIAA1215  521 aa
MDTESQYSGYSYKSGHSRSSRKHRDRRDRHRSKSRDGGRGDKSVTIQAPGEPLLDNESTR
GDERDDNWGETTTVVTGTSEHSISHDDLTRIAKDMEDSVPLDCSRHLGVAAGATLALLSF
LTPLAFLLLPPLLWREELEPCGTACEGLFISVAFKLLILLLGSWALFFRRPKASLPRVFV
LRALLMVLVFLLVVSYWLFYGVRILDARERSYQGVVQFAVSLVDALLFVHYLAVVLLELR
QLQPQFTLKVVRSTDGASRFYNVGHLSIQRVAVWILEKYYHDFPVYNPALLNLPKSVLAK
KVSGFKVYSLGEENSTNNSTGQSRAVIAAAARRRDNSHNEYYYEEAEHERRVRKRRARLV
VAVEEAFTHIKRLQEEEQKNPREVMDPREAAQAIFASMARAMQKYLRTTKQQPYHTMESI
LQHLEFCITHDMTPKAFLERYLAAGPTIQYHKERWLAKQWTLVSEEPVTNGLKDGIVFLL
KRQDFSLVVSTKKVPFFKLSEEFVDPKSHKFVMRLQSETSV

ORF+Vector

Nucleotide Sequence (with vector) for pFN21ASDA1215 Download

>pFN21ASDA1215  6292 bp
TCAATATTGGCCATTAGCCATATTATTCATTGGTTATATAGCATAAATCAATATTGGCTA
TTGGCCATTGCATACGTTGTATCTATATCATAATATGTACATTTATATTGGCTCATGTCC
AATATGACCGCCATGTTGGCATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGG
GTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCC
GCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCAT
AGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGC
CCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTCCGCCCCCTATTGACGTCAATGA
CGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTACGGGACTTTCCTACTTG
GCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACAC
CAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGT
CAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAATAACCC
CGCCCCGTTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGC
TGGTTTAGTGAACCGTCAGATCACTAGAAGCTTTATTGCGGTAGTTTATCACAGTTAAAT
TGCTAACGCAGTCAGTGCTTCTGACACAACAGTCTCGAACTTAAGCTGCAGAAGTTGGTC
GTGAGGCACTGGGCAGGTAAGTATCAAGGTTACAAGACAGGTTTAAGGAGACCAATAGAA
ACTGGGCTTGTCGAGACAGAGAAGACTCTTGCGTTTCTGATAGGCACCTATTGGTCTTAC
TGACATCCACTTTGCCTTTCTCTCCACAGGTGTCCACTCCCAGTTCAATTACAGCTCTTA
AGGCTAGAGTATTAATACGACTCACTATAGGGCTAGCAAAGCCACCATGGCAGAAATCGG
TACTGGCTTTCCATTCGACCCCCATTATGTGGAAGTCCTGGGCGAGCGCATGCACTACGT
CGATGTTGGTCCGCGCGATGGCACCCCTGTGCTGTTCCTGCACGGTAACCCGACCTCCTC
CTACGTGTGGCGCAACATCATCCCGCATGTTGCACCGACCCATCGCTGCATTGCTCCAGA
CCTGATCGGTATGGGCAAATCCGACAAACCAGACCTGGGTTATTTCTTCGACGACCACGT
CCGCTTCATGGATGCCTTCATCGAAGCCCTGGGTCTGGAAGAGGTCGTCCTGGTCATTCA
CGACTGGGGCTCCGCTCTGGGTTTCCACTGGGCCAAGCGCAATCCAGAGCGCGTCAAAGG
TATTGCATTTATGGAGTTCATCCGCCCTATCCCGACCTGGGACGAATGGCCAGAATTTGC
CCGCGAGACCTTCCAGGCCTTCCGCACCACCGACGTCGGCCGCAAGCTGATCATCGATCA
GAACGTTTTTATCGAGGGTACGCTGCCGATGGGTGTCGTCCGCCCGCTGACTGAAGTCGA
GATGGACCATTACCGCGAGCCGTTCCTGAATCCTGTTGACCGCGAGCCACTGTGGCGCTT
CCCAAACGAGCTGCCAATCGCCGGTGAGCCAGCGAACATCGTCGCGCTGGTCGAAGAATA
CATGGACTGGCTGCACCAGTCCCCTGTCCCGAAGCTGCTGTTCTGGGGCACCCCAGGCGT
TCTGATCCCACCGGCCGAAGCCGCTCGCCTGGCCAAAAGCCTGCCTAACTGCAAGGCTGT
GGACATCGGCCCGGGTCTGAATCTGCTGCAAGAAGACAACCCGGACCTGATCGGCAGCGA
GATCGCGCGCTGGCTGTCGACGCTCGAGATTTCCGGCGAGCCAACCACTGAGGATCTGTA
CTTTCAGAGCGATAACGCGATCGCTTTCGAAGGAGATAGAACCATGGACACCGAGTCCCA
GTACTCGGGCTATTCCTACAAGTCGGGCCACTCCCGCAGCTCCCGCAAGCACAGGGACCG
CCGGGACCGACACCGCTCTAAGAGTCGAGATGGGGGCCGAGGGGACAAGTCGGTGACAAT
CCAGGCTCCCGGGGAGCCCCTGCTGGACAATGAGTCCACACGAGGGGATGAGCGGGATGA
CAACTGGGGGGAAACGACGACAGTAGTAACGGGCACCTCAGAGCACAGCATCTCCCATGA
TGACCTCACACGCATCGCCAAGGACATGGAGGACAGTGTCCCTCTGGACTGCTCCCGTCA
CCTGGGTGTGGCAGCGGGGGCCACCCTGGCACTGCTGTCTTTCCTCACGCCTCTGGCCTT
CCTGCTGCTGCCCCCACTGCTGTGGCGGGAGGAGCTGGAGCCTTGCGGGACGGCCTGCGA
GGGCCTCTTCATCTCTGTCGCCTTCAAGCTGCTCATCCTGCTACTGGGCAGCTGGGCTCT
GTTCTTCCGCCGGCCCAAGGCCTCGCTGCCCCGCGTCTTTGTGCTGCGTGCCCTGCTTAT
GGTGCTGGTTTTCCTGCTCGTGGTCTCCTACTGGCTCTTCTATGGTGTGCGCATCCTGGA
TGCTCGGGAGCGCAGCTACCAGGGCGTGGTGCAGTTCGCCGTGTCGCTGGTGGACGCCCT
TCTTTTCGTGCACTACCTGGCCGTGGTCCTGCTGGAGCTGCGCCAGCTCCAGCCTCAGTT
CACGCTCAAGGTCGTGCGCTCCACCGACGGCGCCAGCCGCTTCTACAACGTTGGCCATCT
CAGCATCCAGCGCGTGGCAGTGTGGATCCTGGAGAAGTATTACCATGACTTCCCTGTCTA
CAACCCTGCCCTCCTCAACCTGCCCAAGTCCGTCCTGGCCAAGAAAGTGTCTGGCTTCAA
GGTGTATTCCCTCGGAGAGGAAAACAGCACCAACAACTCCACTGGCCAGTCTCGGGCTGT
GATTGCAGCGGCAGCTCGGAGGCGGGACAACAGTCACAATGAGTACTACTATGAGGAGGC
TGAGCATGAGCGAAGGGTGCGCAAGAGGAGGGCCAGGCTTGTAGTGGCGGTGGAGGAGGC
CTTCACTCACATTAAGCGGCTGCAGGAAGAGGAGCAGAAAAACCCCAGGGAGGTGATGGA
CCCCCGGGAGGCAGCCCAAGCCATCTTTGCATCCATGGCCCGTGCCATGCAGAAGTACCT
TCGGACCACCAAGCAGCAGCCCTACCACACCATGGAGAGCATCCTGCAGCACCTTGAATT
CTGCATCACGCATGACATGACGCCCAAGGCCTTCTTGGAGCGATACTTGGCGGCTGGACC
TACCATCCAGTACCACAAGGAACGCTGGCTGGCCAAACAGTGGACATTGGTGAGCGAGGA
GCCGGTGACCAACGGCCTCAAGGATGGCATCGTTTTCCTCTTAAAACGCCAGGACTTCAG
CCTGGTGGTCAGCACCAAGAAGGTCCCATTCTTCAAACTCTCCGAGGAATTTGTGGATCC
CAAGTCACACAAGTTTGTCATGAGGCTGCAGTCTGAGACCTCAGTGTACGTAGTTTAAAC
GAATTCGGGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCAAGCTGATC
CGGCTGCTAACAAAGCCCGAAAGGAAGCTGAGTTGGCTGCTGCCACCGCTGAGCAATAAC
TAGCATAACCCCTTGGGGCGGCCGCTTCGAGCAGACATGATAAGATACATTGATGAGTTT
GGACAAACCACAACTAGAATGCAGTGAAAAAAATGCTTTATTTGTGAAATTTGTGATGCT
ATTGCTTTATTTGTAACCATTATAAGCTGCAATAAACAAGTTAACAACAACAATTGCATT
CATTTTATGTTTCAGGTTCAGGGGGAGATGTGGGAGGTTTTTTTAAGCAAGTAAAACCTC
TACAAATGTGGTAAAATCGAATTCTAATGGATCCTCTTTGCGCTTGCGTTTTCCCTTGTC
CAGATAGCCCAGTAGCTGACATTCATCCGGGGTCAGCACCGTTTCTGCGGACTGGCTTTC
TACGTAATGGTTTCTTAGACGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCT
ATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGA
TAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCC
CTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTG
AAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTC
AACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACT
TTCAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTC
GGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAG
CATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGAT
AACACTGCGGCCAACTTACTTCTGACAACTATCGGAGGACCGAAGGAGCTAACCGCTTTT
TTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAA
GCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGC
AAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATG
GAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATT
GCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCA
GATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGAT
GAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAATTCGAA
ATGACCGACCAAGCGACGCCCAACCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTAT
CCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCA
GGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGC
ATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACC
AGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCG
GATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTA
GGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCG
TTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGAC
ACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAG
GCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGGACAGTAT
TTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGAT
CCGGCAAACAAACCACCGCTGGTAGCGGTGGTTTTTTTGTTTGCAAGCAGCAGATTACGC
GCAGAAAAAAAGGATTTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGT
GGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCT
AGATCCTTTTATAGTCCGGAAATACAGGAACGCACGCTGGATGGCCCTTCGCTGGGATGG
TGAAACCATGAAAAATGGCAGCTTCAGTGGATTAAGTGGGGGTAATGTGGCCTGTACCCT
CTGGTTGCATAGGTATTCATACGGTTAAAATTTATCAGGCGCGATTGCGGCAGTTTTTCG
GGTGGTTTGTTGCCATTTTTACCTGTCTGCTGCCGTGATCGCGCTGAACGCGTTTTAGCG
GTGCGTACAATTAAGGGATTATGGTAAATCCACTTACTGTCTGCCCTCGTAGCCATCGAG
ATAAACCGCAGTACTCCGGCCACGATGCGTCCGGCGTAGAGGATCGAGATCT