KIAA0022

Product ID	ORK00368
Accession No	D14664
Description	CD302 molecule, transcript variant 1
Clone name	ha00480
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3694 bp) Predicted protein sequence (231 aa)
HaloTag ORF Clone	FHC00368
Flexi ORF Clone	FXC00368
Source	Myeloblast cell line (KG-1)

Length: 3694 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2997 bp
Genome contig ID	gi89161199r_160233610
PolyA signal sequence (AATAAA,-24)	+--------+--------+----*----+---- TAAATTTTTTAAATAAAGTTTTATGGGTTAAGCAG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AAGACAACTGTCATACTGAATTTTATTAAAAGTATATATACTTCAAATTC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	2	r	160333610	160362953	6	99.1	Perfect prediction

Length: 231 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q8IX05	7.7e-103	100.0	CD302 antigen; ...	Homo sapiens
BAF83968	1.3e-102	99.6	unnamed protein...	Homo sapiens
AAP79899	2.4e-94	97.7	DEC-205/DCL-1 f...	Homo sapiens
EAX11395	2.4e-94	97.7	hCG2045884, iso...	Homo sapiens
XP_001148026	1.1e-93	96.8	lymphocyte anti...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB014609	8.5e-12	24.2	KIAA0709

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR001304	44	154	PF00059	C-type lectin
HMMSmart	IPR001304	23	153	SM00034	C-type lectin
ProfileScan	IPR001304	31	143	PS50041	C-type lectin

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	2	RAALPALLLPLLGLAAAAVADCP	24	PRIMARY	23
SOSUI2	2	168	ILISALVIASTVILTVLGAIIWF	190	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	CAGGCAGGCTTGTTTACTAC
Primer_r	GCTTTCATTGGTTATCAGTT
PCR product length	201 bp
PCR conditions	95 °C15 sec58 °C60 sec30 cycles