|
Order Kazusa clone(s) from : |
| Product ID | ORK00534 |
|---|---|
| Accession No | AB007886 |
| Description | zinc finger and SCAN domain containing 12 |
| Clone name | hh01274 |
| Vector information | |
| cDNA sequence | DNA sequence (5471 bp) Predicted protein sequence (613 aa) |
|
HaloTag ORF Clone |
FHC00534
|
| Flexi ORF Clone | FXC00534 |
| Source | Human adult brain |
| Rouge ID |
mKIAA0426
by Kazusa Mouse cDNA Project
|
Length: 5471 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | No warning | No warning |
Integrity of 3' end
| Length of 3'UTR | 3511 bp |
|---|---|
| Genome contig ID | gi89161210r_28354711 |
| PolyA signal sequence (AATAAA,-26) |
+----*----+----*----+----*----+---- |
| Flanking genome sequence (100000 - 99951) |
----+----*----+----*----+----*----+----*----+----* |
Ensembl ContigView (Add our DAS server as a DAS source)
| Chr | f/r | start | end | exon | identity | class | |
|---|---|---|---|---|---|---|---|
|
| 6 | r | 28454711 | 28475490 | 7 | 99.1 | Internal No-hit |
Length: 613 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
|
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| BlastProDom | IPR007087 | 283 | 306 | PD000003 | Zinc finger |
| IPR007087 | 311 | 334 | PD000003 | Zinc finger | |
| IPR007087 | 339 | 362 | PD000003 | Zinc finger | |
| IPR007087 | 367 | 390 | PD000003 | Zinc finger | |
| IPR007087 | 395 | 418 | PD000003 | Zinc finger | |
| IPR007087 | 423 | 445 | PD000003 | Zinc finger | |
| IPR007087 | 451 | 473 | PD000003 | Zinc finger | |
| IPR007087 | 478 | 501 | PD000003 | Zinc finger | |
| IPR007087 | 506 | 529 | PD000003 | Zinc finger | |
| IPR007087 | 534 | 557 | PD000003 | Zinc finger | |
| HMMPfam | IPR003309 | 49 | 144 | PF02023 | Transcriptional regulator SCAN |
| IPR007087 | 283 | 305 | PF00096 | Zinc finger | |
| IPR007087 | 311 | 333 | PF00096 | Zinc finger | |
| IPR007087 | 339 | 361 | PF00096 | Zinc finger | |
| IPR007087 | 367 | 389 | PF00096 | Zinc finger | |
| IPR007087 | 395 | 417 | PF00096 | Zinc finger | |
| IPR007087 | 423 | 445 | PF00096 | Zinc finger | |
| IPR007087 | 451 | 472 | PF00096 | Zinc finger | |
| IPR007087 | 478 | 500 | PF00096 | Zinc finger | |
| IPR007087 | 506 | 528 | PF00096 | Zinc finger | |
| IPR007087 | 534 | 556 | PF00096 | Zinc finger | |
| HMMSmart | IPR003309 | 51 | 163 | SM00431 | Transcriptional regulator SCAN |
| IPR015880 | 283 | 305 | SM00355 | Zinc finger | |
| IPR015880 | 311 | 333 | SM00355 | Zinc finger | |
| IPR015880 | 339 | 361 | SM00355 | Zinc finger | |
| IPR015880 | 367 | 389 | SM00355 | Zinc finger | |
| IPR015880 | 395 | 417 | SM00355 | Zinc finger | |
| IPR015880 | 423 | 445 | SM00355 | Zinc finger | |
| IPR015880 | 451 | 472 | SM00355 | Zinc finger | |
| IPR015880 | 478 | 500 | SM00355 | Zinc finger | |
| IPR015880 | 506 | 528 | SM00355 | Zinc finger | |
| IPR015880 | 534 | 556 | SM00355 | Zinc finger | |
| ProfileScan | IPR003309 | 55 | 137 | PS50804 | Transcriptional regulator SCAN |
| IPR007087 | 283 | 310 | PS50157 | Zinc finger | |
| IPR007087 | 311 | 338 | PS50157 | Zinc finger | |
| IPR007087 | 339 | 366 | PS50157 | Zinc finger | |
| IPR007087 | 367 | 394 | PS50157 | Zinc finger | |
| IPR007087 | 395 | 422 | PS50157 | Zinc finger | |
| IPR007087 | 423 | 450 | PS50157 | Zinc finger | |
| IPR007087 | 451 | 477 | PS50157 | Zinc finger | |
| IPR007087 | 478 | 505 | PS50157 | Zinc finger | |
| IPR007087 | 506 | 533 | PS50157 | Zinc finger | |
| IPR007087 | 534 | 561 | PS50157 | Zinc finger | |
| IPR007087 | 562 | 592 | PS50157 | Zinc finger | |
| ScanRegExp | IPR007087 | 285 | 305 | PS00028 | Zinc finger |
| IPR007087 | 313 | 333 | PS00028 | Zinc finger | |
| IPR007087 | 341 | 361 | PS00028 | Zinc finger | |
| IPR007087 | 369 | 389 | PS00028 | Zinc finger | |
| IPR007087 | 397 | 417 | PS00028 | Zinc finger | |
| IPR007087 | 425 | 446 | PS00028 | Zinc finger | |
| IPR007087 | 480 | 500 | PS00028 | Zinc finger | |
| IPR007087 | 508 | 528 | PS00028 | Zinc finger | |
| IPR007087 | 536 | 556 | PS00028 | Zinc finger |
RT-PCR
|
|---|
Experimental conditions| Primer_f | TGGTTCCTGTATTTAGTGCCC |
|---|---|
| Primer_r | CAACAAGTTGATAATGTAGCC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. 6
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | TGGTTCCTGTATTTAGTGCCC |
| Primer_r | CAACAAGTTGATAATGTAGCC |
| PCR product length | 179 bp |
| PCR conditions | 95 °C 15 sec 60 °C 60 sec 30 cycles |