Gene/Protein Characteristic Table for KIAA0548
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Product ID ORK04232
Accession No AB011120
Description attractin
Clone name hh00769
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5632 bp)
Predicted protein sequence (452 aa)
Source Human adult brain
Rouge ID mKIAA0548 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 5632 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Features of the protein sequence
Description

Length: 452 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
EAX10531 4e-194 100.0 attractin, isof...
Homo sapiens
O75882 4.2e-194 100.0 Attractin; Maho...
Homo sapiens
XP_001115192 7.4e-194 99.8 attractin [Maca...
Macaca mulatta
AAF72881 8.6e-194 99.8 membrane attrac...
Homo sapiens
AAM95445 2.1e-192 98.7 attractin [Bos ...
Bos taurus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB011106 5.4e-110 68.0 KIAA0534
AB011542 7.1e-08 30.5 KIAA0818
AB058676 1.8e-05 26.0 KIAA1780
AB011541 3.3e-05 31.6 KIAA0817
AB011539 3.7e-05 26.6 KIAA0815
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR002165 9 84 PF01437 Plexin
IPR002049 86 129 PF00053 EGF-like
IPR002049 132 178 PF00053 EGF-like
HMMSmart IPR003659 9 84 SM00423 Plexin/semaphorin/integrin
IPR002049 86 129 SM00180 EGF-like
IPR002049 132 178 SM00180 EGF-like
ProfileScan IPR002049 86 131 PS50027 EGF-like
IPR002049 132 180 PS50027 EGF-like
ScanRegExp IPR002049 100 134 PS01248 EGF-like

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 273 NITFFVYVSNFTWPIKIQIAFSQ 295 SECONDARY 23
2 301 DLVQFFVTFFSCFLSLLLVAAVV 323 PRIMARY 23
Expression profile
Description

RT-PCR
Experimental conditions
Primer_f AAATGCACAGACCTCCCTTAG
Primer_r ATTTCATTGCGGGGATTGTGG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 20
Experimental conditions
Panel name GeneBridge 4
Primer_f AAATGCACAGACCTCCCTTAG
Primer_r ATTTCATTGCGGGGATTGTGG
PCR product length 126 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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