KIAA0727

Product ID	ORK01115
Accession No	AB018270
Description	myosin ID, transcript variant 1
Clone name	hk03490y1
Vector information	The cDNA fragment was originally inserted at BssH II-NotI si ... The cDNA fragment was originally inserted at BssH II-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The adjacent sequence to the 5' end of the cDNA is as follows: GCGCGCTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GGCCCCCCCT CGAGGTCGAC CCACGCGTCC GTAATACGAC TCACTATAGG GCGAATTGGG TACCGGGCCC CCCCTCGAGG TCGACCCACG CGTCCGTAAT ACGACTCACT ATAGGGCGAA TTGGGTACCG GGCCCCCCCT CGAGGTCGAC CCACGCGTAA TACGACTCAC TATAGGGCGA ATTGGGTACC GGGCCCCCCC TCGAGGTCGA CCCACGCGTC CG-5' end of cDNA.
cDNA sequence	DNA sequence (5182 bp) Predicted protein sequence (1010 aa)
HaloTag ORF Clone	FHC01115
Flexi ORF Clone	FXC01115
Source	Human adult brain
Note	We replaced hk03490, former representative clones for KIAA0727 with hk03490y1. (2002/12/27)

Length: 5182 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	2149 bp
Genome contig ID	gi51511734r_27743741
PolyA signal sequence (AATAAA,-18)	+--------+--------+----*----+---- GATTTGCCATCAAGAGCAATAAAGCATTAAATCTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ATTTTTAAAGGTGTTCTGCTTATCTGTCACTGAAAACTTGGTGAAATTTT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	17	r	27843741	28228015	22	99.5	Perfect prediction

Length: 1010 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
EAW80221	100.0	myosin ID, isof...	Homo sapiens
XP_511404	99.6	myosin ID [Pan ...	Pan troglodytes
O94832	100.0	Myosin-Id.	Homo sapiens
XP_548273	98.4	similar to Myos...	Canis lupus fam...
Q63357	98.0	Myosin-Id; Myos...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB023217	5.4e-81	33.6	KIAA1000
AB020673	1e-74	35.8	KIAA0866
AB040945	9e-70	32.3	KIAA1512
AB032945	6.1e-69	34.5	KIAA1119
AB018342	4e-58	35.2	KIAA0799

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR001609	142	169	PD000355	Myosin head
FPrintScan	IPR001609	43	62	PR00193	Myosin head
	IPR001609	99	124	PR00193	Myosin head
	IPR001609	147	174	PR00193	Myosin head
	IPR001609	382	410	PR00193	Myosin head
	IPR001609	435	463	PR00193	Myosin head
HMMPfam	IPR001609	17	686	PF00063	Myosin head
	IPR000048	703	723	PF00612	IQ calmodulin-binding region
	IPR000048	725	745	PF00612	IQ calmodulin-binding region
	IPR010926	806	1009	PF06017	Myosin tail 2
HMMSmart	IPR001609	7	700	SM00242	Myosin head
HMMSmart	IPR000048	701	723	SM00015	IQ calmodulin-binding region
ProfileScan	IPR000048	702	730	PS50096	IQ calmodulin-binding region

Primer_f	TGTGGCAACTGCAAAAGGATC
Primer_r	GTCTACAATCATGCCCGCTTC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	TGTGGCAACTGCAAAAGGATC
Primer_r	GTCTACAATCATGCCCGCTTC
PCR product length	93 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles