KIAA1520

Product ID	ORK01172
Accession No	AB040953
Description	ATP-binding cassette, sub-family B (MDR/TAP), member 9, transcript variant 1
Clone name	ah05473
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6051 bp) Predicted protein sequence (810 aa)
HaloTag ORF Clone	FHC01172
Flexi ORF Clone	FXC01172
Source	Human brain (amygdala)
Rouge ID	mKIAA1520 by Kazusa Mouse cDNA Project
Note	We replaced fh14074, former representative clones for KIAA1520 with ah05473. (2001/2/22)

Length: 6051 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	911 bp
Genome contig ID	gi89161190r_121879500
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- TGAGAGAACCCGGTCAATAAAGTGTACTACCTCTT
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACCCCTAACCTCTGACTTCTACTTGGGCCAACTGCCTCATCGCCCAGTTA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	r	121979500	122032149	12	99.5	Perfect prediction

Length: 810 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
XP_001096136	98.5	ATP-binding cas...	Macaca mulatta
Q9NP78	100.0	ATP-binding cas...	Homo sapiens
AAH64384	99.6	ABCB9 protein [...	Homo sapiens
XP_858668	93.3	similar to ATP-...	Canis lupus fam...
Q9JJ59	93.7	ATP-binding cas...	Mus musculus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB028985	2e-05	23.4	KIAA1062
AB067475	2.9e-05	22.4	KIAA1888

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
BlastProDom	IPR003439	686	729	PD000006	ABC transporter related
HMMPfam	IPR001140	232	503	PF00664	ABC transporter
HMMPfam	IPR003439	576	760	PF00005	ABC transporter related
HMMSmart	IPR003593	575	761	SM00382	AAA+ ATPase
ProfileScan	IPR011527	233	515	PS50929	ABC transporter
ProfileScan	IPR003439	548	784	PS50893	ABC transporter related
ScanRegExp	IPR003439	687	701	PS00211	ABC transporter related

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	49	KAVVVTLAFMSVDICVTTAIYVF	71	PRIMARY	23
	2	91	VLDLWAACLYRSCLLLGATIGVA	113	SECONDARY	23
	3	128	LVITLVCLFVGIYAMVKLLLFSE	150	PRIMARY	23
	4	159	WFWALFVWTYISLGASFLLWWLL	181	PRIMARY	23
	5	227	PDVAFLVAASFFLIVAALGETFL	249	PRIMARY	23
	6	260	IVIQKSMDQFSTAVVIVCLLAIG	282	SECONDARY	23
	7	363	VFMFSLSWQLSLVTFMGFPIIMM	385	PRIMARY	23
	8	468	QVSILYYGGHLVISGQMTSGNLI	490	PRIMARY	23