KIAA1531

Product ID	ORK01174
Accession No	AB040964
Description	adhesion G protein-coupled receptor A2
Clone name	ph00937
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector. This cDNA clone ph00937 has an irregular adapter sequence (GTCGACCCAC GCGTCCGCCC ACGCGTCCG) instead of regular one (GTCGACCCAC GCGTCCG).
cDNA sequence	DNA sequence (5350 bp) Predicted protein sequence (1206 aa)
HaloTag ORF Clone	FHC01174
Flexi ORF Clone	FXC01174
Source	Human brain (hippocampus)
Rouge ID	mKIAA1531 by Kazusa Mouse cDNA Project
Note	We replaced hj08219, former representative clones for KIAA1531 with ph00937. (2002/5/10)

Length: 5350 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	1621 bp
Genome contig ID	gi51511724f_37673582
PolyA signal sequence (ATTAAA,-32)	+--------+--------+----*----+---- TATATTAAAATTTTGCAAAGCCCTTTGAGCTACTG
Flanking genome sequence (147072 - 147121)	----+--------+--------+--------+--------+----* CCTTAGTCTACCCACTGTCCTTTTGTTATGAGGTAGAGGATCTCATGACA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	8	f	37773582	37820652	16	99.5	Perfect prediction

Length: 1206 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAG11335	0	100.0	G-protein coupl...	synthetic construct
EAW63357	0	99.9	G protein-coupl...	Homo sapiens
AAI46775	0	100.0	GPR124 protein ...	Homo sapiens
BAB84925	2.2e-195	83.2	FLJ00170 protei...	Homo sapiens
AAL11992	2.2e-195	83.6	tumor endotheli...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB058731	4.9e-10	39.2	KIAA1828
D86983	3e-06	28.4	KIAA0230
AB011538	1.2e-05	33.0	KIAA0814
AB011537	1.5e-05	27.1	KIAA0813
AB040898	7.9e-05	31.5	KIAA1465

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
FPrintScan	IPR001611	195	208	PR00019	Leucine-rich repeat
FPrintScan	IPR001611	216	229	PR00019	Leucine-rich repeat
HMMPfam	IPR001611	170	192	PF00560	Leucine-rich repeat
	IPR001611	194	216	PF00560	Leucine-rich repeat
	IPR001611	218	240	PF00560	Leucine-rich repeat
	IPR001611	242	264	PF00560	Leucine-rich repeat
	IPR001879	452	506	PF02793	Hormone receptor
HMMSmart	IPR003591	167	191	SM00369	Leucine-rich repeat
	IPR003591	192	215	SM00369	Leucine-rich repeat
	IPR003591	216	239	SM00369	Leucine-rich repeat
	IPR003591	240	263	SM00369	Leucine-rich repeat
	IPR000483	275	325	SM00082	Cysteine-rich flanking region
	IPR003599	338	431	SM00409	Immunoglobulin subtype
ProfileScan	IPR007110	345	429	PS50835	Immunoglobulin-like
	IPR001879	412	506	PS50227	Hormone receptor
	IPR000832	637	812	PS50261	GPCR
ScanRegExp	IPR000832	929	944	PS00650	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	637	LHPVVYPCTALLLLCLFATIITY	659	PRIMARY	23
	2	674	HMLLNLCFHIAMTSAVFAGGITL	696	SECONDARY	23
	3	751	PMLRFYLIAGGIPLIICGITAAV	773	SECONDARY	23
	4	796	FYIPVALILLITWIYFLCAGLR	817	PRIMARY	22
	5	884	GVQLGALVTTHFLYLAMWACGAL	906	SECONDARY	23
	6	916	VVCSCLYGVAASALGLFVFTHHC	938	PRIMARY	23

Primer_f	TCATTTGATCTCCAGCTCCAC
Primer_r	TGGTCCTGTAGTGAAATGCTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-