KIAA1828

Product ID	ORK05341
Accession No	AB058731
Description	adhesion G protein-coupled receptor A1
Clone name	ha06249
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pSPORT1 vector (GenBank Accession No. U12390, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3536 bp) Predicted protein sequence (496 aa)
Source	Human adult brain

Length: 3536 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	2015 bp
Genome contig ID	gi89161187f_134665740
PolyA signal sequence (AATACA,-20)	+--------+--------+----*----+---- AACCGTCCACACAAAAATACAGTCTTGGCATTGTT
Flanking genome sequence (129184 - 129233)	----+--------+--------+--------+--------+----* TGTTCTTTGTGAGGCTGGTTAATAGCATCCCCGTGTGTTTTTCTCACCCT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	10	f	134765740	134794922	5	99.5	Internal No-hit

Length: 496 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
ABQ08572	1.8e-195	100.0	G protein-coupl...	Homo sapiens
EAW61312	3.3e-195	100.0	G protein-coupl...	Homo sapiens
Q86SQ6	5.1e-194	99.8	Probable G-prot...	Homo sapiens
EAW61313	5.1e-194	99.8	G protein-coupl...	Homo sapiens
AAO27353	5.1e-194	99.8	G protein-coupl...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB040964	1.1e-13	39.0	KIAA1531

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
ProfileScan	IPR000832	22	246	PS50261	GPCR
ScanRegExp	IPR000832	234	249	PS00650	GPCR

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	19	PIQVGIVLHYSTLSTMLWIGVTA	41	SECONDARY	23
	2	68	LLRFYLVSGGVPFIICGVTAATN	90	SECONDARY	23
	3	115	FYGPAAIITLVTCVYFLGTYVQ	136	PRIMARY	22
	4	192	LRAAAFTLFLFTATWAFGALAVS	214	SECONDARY	23
	5	221	MVFSCLYGAFCVTLGLFVLIHHC	243	PRIMARY	23

Primer_f	ATCTACAAGCAGGTGACCAAG
Primer_r	TCCTCTGTCCCGTAATTCCTG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	genbank
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-