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Order Kazusa clone(s) from : |
| Product ID | ORK05475 |
|---|---|
| Accession No | AB046798 |
| Description | HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase |
| Clone name | fj04270 |
| Vector information | |
| cDNA sequence | DNA sequence (3670 bp) Predicted protein sequence (1202 aa) |
| Source | Human fetal brain |
| Note | Please refer to "Gene/Protein Characteristic Table for KIAA0312" because the cDNA sequence of KIAA1578 is included in KIAA0312. |
Length: 3670 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Length: 1202 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR000449 | 72 | 110 | PF00627 | Ubiquitin-associated/Translation elongation factor EF1B |
| IPR004170 | 364 | 435 | PF02825 | WWE | |
| HMMSmart | IPR000449 | 73 | 109 | SM00165 | Ubiquitin-associated/Translation elongation factor EF1B |
| ProfileScan | IPR000449 | 71 | 110 | PS50030 | Ubiquitin-associated/Translation elongation factor EF1B |
| IPR004170 | 358 | 435 | PS50918 | WWE |
RT-PCR-ELISA
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Experimental conditions| Primer_f | AACATCATTCGGCTTTTCCTG |
|---|---|
| Primer_r | TACTGGGCTGGTTCACAATCC |
| PCR conditions | 95 °C 30 sec 55 °C 30 sec 72 °C 60 sec 30 cycles![]() |
Chromosome No. X
Experimental conditions| Panel name | CCR |
|---|---|
| Primer_f | CAGAGCAGTGACTTTGATACG |
| Primer_r | GAGGTAGGCATTAAAGGTTTG |
| PCR product length | 218(1.6k) bp |
| PCR conditions | 95 °C 15 sec 62 °C 60 sec 30 cycles |