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Order Kazusa clone(s) from : |
| Product ID | ORK05590 |
|---|---|
| Accession No | AB002310 |
| Description | HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase |
| Clone name | ff01724c1 |
| Vector information | |
| cDNA sequence | DNA sequence (10790 bp) Predicted protein sequence (3192 aa) |
| Source | Human fetal brain |
| Rouge ID |
mKIAA0312
by Kazusa Mouse cDNA Project
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| Note | We replaced ff01724 and hg00137, former representative clones for KIAA0312 with ff01724c1. (2011/09/22,2003/8/28) |
Length: 10790 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).
| N-terminal truncation | Coding interruption | |
|---|---|---|
| cloned DNA seq | Warning | No warning |
Length: 3192 aa
Result of homology search against nr database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database
(FASTA output,
Multiple alignment)![]() |
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The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)
Result of InterProScan
| Search method | interpro_ID | From | To | Entry | Definition |
|---|---|---|---|---|---|
| HMMPfam | IPR000449 | 152 | 190 | PF00627 | Ubiquitin-associated/Translation elongation factor EF1B |
| IPR004170 | 444 | 515 | PF02825 | WWE | |
| IPR000569 | 2885 | 3192 | PF00632 | HECT | |
| HMMSmart | IPR000449 | 153 | 189 | SM00165 | Ubiquitin-associated/Translation elongation factor EF1B |
| IPR000569 | 2854 | 3192 | SM00119 | HECT | |
| ProfileScan | IPR000449 | 151 | 190 | PS50030 | Ubiquitin-associated/Translation elongation factor EF1B |
| IPR004170 | 438 | 515 | PS50918 | WWE | |
| IPR000569 | 2856 | 3192 | PS50237 | HECT |
RT-PCR
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|---|
Experimental conditions| Primer_f | AGCCCAAAGCCTTCAGAGCAC |
|---|---|
| Primer_r | ATCGAGATGACAGGTCCACAG |
| PCR conditions | 95 °C 15 sec 64 °C 15 sec 72 °C 45 sec 30 cycles![]() |
Chromosome No. X
Experimental conditions| Panel name | GeneBridge 4 |
|---|---|
| Primer_f | AGCCCAAAGCCTTCAGAGCAC |
| Primer_r | ATCGAGATGACAGGTCCACAG |
| PCR product length | 141 (0.8k) bp |
| PCR conditions | 95 °C 15 sec 66 °C 60 sec 30 cycles |